G2/M cell cycle arrest on HT-29 cancer cells and toxicity assessment of triphenylphosphanegold(I) carbonimidothioates, Ph3PAu[SC(OR) = NPh], R = Me, Et, and iPr, during zebrafish development
Phosphanegold(I) thiolates, Ph3PAu[SC(OR) = NPh], R = Me (1), Et (2) and iPr (3), were previously shown to be significantly cytotoxic toward HT-29 cancer cells and to induce cell death by both intrinsic and extrinsic apoptotic pathways whereby 1 activated the p73 gene, and each of 2 and 3 activated...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/61930/1/Cell%20cycle%20arrest%20on%20HT-29%20cancer%20cells%20and%20toxicity%20assessment%20.pdf |
| _version_ | 1825932411918090240 |
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| author | Ooi, Kah Kooi Yeo, Chien Ing Mahandaran, Theventhiran Ang, Kok Pian Md Akim, Abdah Cheah, Yoke Kqueen Seng, Hoi Ling Tiekink, Edward R.T. |
| author_facet | Ooi, Kah Kooi Yeo, Chien Ing Mahandaran, Theventhiran Ang, Kok Pian Md Akim, Abdah Cheah, Yoke Kqueen Seng, Hoi Ling Tiekink, Edward R.T. |
| author_sort | Ooi, Kah Kooi |
| collection | UPM |
| description | Phosphanegold(I) thiolates, Ph3PAu[SC(OR) = NPh], R = Me (1), Et (2) and iPr (3), were previously shown to be significantly cytotoxic toward HT-29 cancer cells and to induce cell death by both intrinsic and extrinsic apoptotic pathways whereby 1 activated the p73 gene, and each of 2 and 3 activated p53; 2 also caused apoptotic cell death via the c-Jun N-terminal kinase/mitogen-activated protein kinase pathway. Apoptosis pathways have been further evaluated by mitochondrial cytochrome c measurements and annexin V screening, confirming apoptotic pathways of cell death. Cell cycle analysis showed the majority of treated HT-29 cells were arrested at the G2/M checkpoint after 24 h; results of both assays were confirmed by changes in populations of relevant genes (PCR array analysis). Cell invasion studies showed inhibition of metastasis through Matrigel™ matrix to 17–22% cf. untreated cells. LC50 values were determined in zebrafish (8.36, 8.17, and 7.64 μM for 1–3). Finally, the zebrafish tolerated doses of 1 and 2 up to 0.625 μM, and 3 was tolerated at even higher doses of up to 1.25 μM. |
| first_indexed | 2024-03-06T09:41:37Z |
| format | Article |
| id | upm.eprints-61930 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:41:37Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-619302019-02-27T08:48:54Z http://psasir.upm.edu.my/id/eprint/61930/ G2/M cell cycle arrest on HT-29 cancer cells and toxicity assessment of triphenylphosphanegold(I) carbonimidothioates, Ph3PAu[SC(OR) = NPh], R = Me, Et, and iPr, during zebrafish development Ooi, Kah Kooi Yeo, Chien Ing Mahandaran, Theventhiran Ang, Kok Pian Md Akim, Abdah Cheah, Yoke Kqueen Seng, Hoi Ling Tiekink, Edward R.T. Phosphanegold(I) thiolates, Ph3PAu[SC(OR) = NPh], R = Me (1), Et (2) and iPr (3), were previously shown to be significantly cytotoxic toward HT-29 cancer cells and to induce cell death by both intrinsic and extrinsic apoptotic pathways whereby 1 activated the p73 gene, and each of 2 and 3 activated p53; 2 also caused apoptotic cell death via the c-Jun N-terminal kinase/mitogen-activated protein kinase pathway. Apoptosis pathways have been further evaluated by mitochondrial cytochrome c measurements and annexin V screening, confirming apoptotic pathways of cell death. Cell cycle analysis showed the majority of treated HT-29 cells were arrested at the G2/M checkpoint after 24 h; results of both assays were confirmed by changes in populations of relevant genes (PCR array analysis). Cell invasion studies showed inhibition of metastasis through Matrigel™ matrix to 17–22% cf. untreated cells. LC50 values were determined in zebrafish (8.36, 8.17, and 7.64 μM for 1–3). Finally, the zebrafish tolerated doses of 1 and 2 up to 0.625 μM, and 3 was tolerated at even higher doses of up to 1.25 μM. Elsevier 2017-01 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/61930/1/Cell%20cycle%20arrest%20on%20HT-29%20cancer%20cells%20and%20toxicity%20assessment%20.pdf Ooi, Kah Kooi and Yeo, Chien Ing and Mahandaran, Theventhiran and Ang, Kok Pian and Md Akim, Abdah and Cheah, Yoke Kqueen and Seng, Hoi Ling and Tiekink, Edward R.T. (2017) G2/M cell cycle arrest on HT-29 cancer cells and toxicity assessment of triphenylphosphanegold(I) carbonimidothioates, Ph3PAu[SC(OR) = NPh], R = Me, Et, and iPr, during zebrafish development. Journal of Inorganic Biochemistry, 166. pp. 173-181. ISSN 0162-0134; ESSN: 1873-3344 https://www.sciencedirect.com/science/article/pii/S0162013416303798 10.1016/j.jinorgbio.2016.11.008 |
| spellingShingle | Ooi, Kah Kooi Yeo, Chien Ing Mahandaran, Theventhiran Ang, Kok Pian Md Akim, Abdah Cheah, Yoke Kqueen Seng, Hoi Ling Tiekink, Edward R.T. G2/M cell cycle arrest on HT-29 cancer cells and toxicity assessment of triphenylphosphanegold(I) carbonimidothioates, Ph3PAu[SC(OR) = NPh], R = Me, Et, and iPr, during zebrafish development |
| title | G2/M cell cycle arrest on HT-29 cancer cells and toxicity assessment of triphenylphosphanegold(I) carbonimidothioates, Ph3PAu[SC(OR) = NPh], R = Me, Et, and iPr, during zebrafish development |
| title_full | G2/M cell cycle arrest on HT-29 cancer cells and toxicity assessment of triphenylphosphanegold(I) carbonimidothioates, Ph3PAu[SC(OR) = NPh], R = Me, Et, and iPr, during zebrafish development |
| title_fullStr | G2/M cell cycle arrest on HT-29 cancer cells and toxicity assessment of triphenylphosphanegold(I) carbonimidothioates, Ph3PAu[SC(OR) = NPh], R = Me, Et, and iPr, during zebrafish development |
| title_full_unstemmed | G2/M cell cycle arrest on HT-29 cancer cells and toxicity assessment of triphenylphosphanegold(I) carbonimidothioates, Ph3PAu[SC(OR) = NPh], R = Me, Et, and iPr, during zebrafish development |
| title_short | G2/M cell cycle arrest on HT-29 cancer cells and toxicity assessment of triphenylphosphanegold(I) carbonimidothioates, Ph3PAu[SC(OR) = NPh], R = Me, Et, and iPr, during zebrafish development |
| title_sort | g2 m cell cycle arrest on ht 29 cancer cells and toxicity assessment of triphenylphosphanegold i carbonimidothioates ph3pau sc or nph r me et and ipr during zebrafish development |
| url | http://psasir.upm.edu.my/id/eprint/61930/1/Cell%20cycle%20arrest%20on%20HT-29%20cancer%20cells%20and%20toxicity%20assessment%20.pdf |
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