Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen
In this study, a disposable and simple electrochemical immunosensor was fabricated for the detection of carcinoembryonic antigen. In this method, silver nanoparticles (AgNPs) were mixed with reduced graphene oxide (rGO) to modify the surface of screen-printed carbon electrode (SPE). Initially, AgNPs...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62039/1/Horseradish%20peroxidase-labeled%20silver.pdf |
| _version_ | 1796977563435794432 |
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| author | Lee, S. X. Lim, Hong Ngee Ibrahim, Izwaharyanie Jamil, Asilah Pandikumar, Alagarsamy Huang, Nay Ming |
| author_facet | Lee, S. X. Lim, Hong Ngee Ibrahim, Izwaharyanie Jamil, Asilah Pandikumar, Alagarsamy Huang, Nay Ming |
| author_sort | Lee, S. X. |
| collection | UPM |
| description | In this study, a disposable and simple electrochemical immunosensor was fabricated for the detection of carcinoembryonic antigen. In this method, silver nanoparticles (AgNPs) were mixed with reduced graphene oxide (rGO) to modify the surface of screen-printed carbon electrode (SPE). Initially, AgNPs-rGO modified-SPEs were fabricated by using simple electrochemical deposition method. Then the carcinoembryonic antigen (CEA) was immobilized between the primary antibody and horseradish peroxidase (HRP)-conjugated secondary antibody onto AgNPs-rGO modified-SPEs to fabricate a sandwich-type electrochemical immunosensor. The proposed method could detect the CEA with a linear range of 0.05–0.50 µg mL−1 and a detection limit down to 0.035 µg mL−1 as compared to its non-sandwich counterpart, which yielded a linear range of 0.05–0.40 µg mL−1, with a detection limit of 0.042 µg mL−1. The immunosensor showed good performance in the detection of carcinoembryonic antigen, exhibiting a simple, rapid and low-cost. The immunosensor showed a higher sensitivity than an enzymeless sensor. |
| first_indexed | 2024-03-06T09:41:54Z |
| format | Article |
| id | upm.eprints-62039 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:41:54Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-620392019-03-20T05:21:10Z http://psasir.upm.edu.my/id/eprint/62039/ Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen Lee, S. X. Lim, Hong Ngee Ibrahim, Izwaharyanie Jamil, Asilah Pandikumar, Alagarsamy Huang, Nay Ming In this study, a disposable and simple electrochemical immunosensor was fabricated for the detection of carcinoembryonic antigen. In this method, silver nanoparticles (AgNPs) were mixed with reduced graphene oxide (rGO) to modify the surface of screen-printed carbon electrode (SPE). Initially, AgNPs-rGO modified-SPEs were fabricated by using simple electrochemical deposition method. Then the carcinoembryonic antigen (CEA) was immobilized between the primary antibody and horseradish peroxidase (HRP)-conjugated secondary antibody onto AgNPs-rGO modified-SPEs to fabricate a sandwich-type electrochemical immunosensor. The proposed method could detect the CEA with a linear range of 0.05–0.50 µg mL−1 and a detection limit down to 0.035 µg mL−1 as compared to its non-sandwich counterpart, which yielded a linear range of 0.05–0.40 µg mL−1, with a detection limit of 0.042 µg mL−1. The immunosensor showed good performance in the detection of carcinoembryonic antigen, exhibiting a simple, rapid and low-cost. The immunosensor showed a higher sensitivity than an enzymeless sensor. Elsevier 2017-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62039/1/Horseradish%20peroxidase-labeled%20silver.pdf Lee, S. X. and Lim, Hong Ngee and Ibrahim, Izwaharyanie and Jamil, Asilah and Pandikumar, Alagarsamy and Huang, Nay Ming (2017) Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen. Biosensors and Bioelectronics, 89 (pt. 1). pp. 673-680. ISSN 0956-5663; ESSN: 1873-4235 https://www.sciencedirect.com/science/article/pii/S0956566315306850?via%3Dihub#! 10.1016/j.bios.2015.12.030 |
| spellingShingle | Lee, S. X. Lim, Hong Ngee Ibrahim, Izwaharyanie Jamil, Asilah Pandikumar, Alagarsamy Huang, Nay Ming Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title | Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title_full | Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title_fullStr | Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title_full_unstemmed | Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title_short | Horseradish peroxidase-labeled silver/reduced graphene oxide thin film-modified screen-printed electrode for detection of carcinoembryonic antigen |
| title_sort | horseradish peroxidase labeled silver reduced graphene oxide thin film modified screen printed electrode for detection of carcinoembryonic antigen |
| url | http://psasir.upm.edu.my/id/eprint/62039/1/Horseradish%20peroxidase-labeled%20silver.pdf |
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