Identification and characterization of Burkholderia multivorans CCA53
Objective: A lignin-degrading bacterium, Burkholderia sp. CCA53, was previously isolated from leaf soil. The purpose of this study was to determine phenotypic and biochemical features of Burkholderia sp. CCA53. Results: Multilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BioMed Central
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62066/1/Identification%20and%20characterization%20of%20Burkholderia%20multivorans%20CCA53.pdf |
| _version_ | 1796977567953059840 |
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| author | Akita, Hironaga Kimura, Zen-ichiro Mohd Yusoff, Mohd Zulkhairi Nakashima, Nobutaka Hoshino, Tamotsu |
| author_facet | Akita, Hironaga Kimura, Zen-ichiro Mohd Yusoff, Mohd Zulkhairi Nakashima, Nobutaka Hoshino, Tamotsu |
| author_sort | Akita, Hironaga |
| collection | UPM |
| description | Objective: A lignin-degrading bacterium, Burkholderia sp. CCA53, was previously isolated from leaf soil. The purpose of this study was to determine phenotypic and biochemical features of Burkholderia sp. CCA53. Results: Multilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD, gyrB, lepA, recA and trpB gene sequences was performed to identify Burkholderia sp. CCA53. The MLST analysis revealed that Burkholderia sp. CCA53 was tightly clustered with B. multivorans ATCC BAA-247T. The quinone and cellular fatty acid profiles, carbon source utilization, growth temperature and pH were consistent with the characteristics of B. multivorans species. Burkholderia sp. CCA53 was therefore identified as B. multivorans CCA53. |
| first_indexed | 2024-03-06T09:41:58Z |
| format | Article |
| id | upm.eprints-62066 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:41:58Z |
| publishDate | 2017 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | upm.eprints-620662019-03-21T09:02:11Z http://psasir.upm.edu.my/id/eprint/62066/ Identification and characterization of Burkholderia multivorans CCA53 Akita, Hironaga Kimura, Zen-ichiro Mohd Yusoff, Mohd Zulkhairi Nakashima, Nobutaka Hoshino, Tamotsu Objective: A lignin-degrading bacterium, Burkholderia sp. CCA53, was previously isolated from leaf soil. The purpose of this study was to determine phenotypic and biochemical features of Burkholderia sp. CCA53. Results: Multilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD, gyrB, lepA, recA and trpB gene sequences was performed to identify Burkholderia sp. CCA53. The MLST analysis revealed that Burkholderia sp. CCA53 was tightly clustered with B. multivorans ATCC BAA-247T. The quinone and cellular fatty acid profiles, carbon source utilization, growth temperature and pH were consistent with the characteristics of B. multivorans species. Burkholderia sp. CCA53 was therefore identified as B. multivorans CCA53. BioMed Central 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62066/1/Identification%20and%20characterization%20of%20Burkholderia%20multivorans%20CCA53.pdf Akita, Hironaga and Kimura, Zen-ichiro and Mohd Yusoff, Mohd Zulkhairi and Nakashima, Nobutaka and Hoshino, Tamotsu (2017) Identification and characterization of Burkholderia multivorans CCA53. BMC Research Notes, 10. pp. 1-6. ISSN 1756-0500 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501517/ 10.1186/s13104-017-2565-1 |
| spellingShingle | Akita, Hironaga Kimura, Zen-ichiro Mohd Yusoff, Mohd Zulkhairi Nakashima, Nobutaka Hoshino, Tamotsu Identification and characterization of Burkholderia multivorans CCA53 |
| title | Identification and characterization of Burkholderia multivorans CCA53 |
| title_full | Identification and characterization of Burkholderia multivorans CCA53 |
| title_fullStr | Identification and characterization of Burkholderia multivorans CCA53 |
| title_full_unstemmed | Identification and characterization of Burkholderia multivorans CCA53 |
| title_short | Identification and characterization of Burkholderia multivorans CCA53 |
| title_sort | identification and characterization of burkholderia multivorans cca53 |
| url | http://psasir.upm.edu.my/id/eprint/62066/1/Identification%20and%20characterization%20of%20Burkholderia%20multivorans%20CCA53.pdf |
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