Isolation and characterization of lectin from the Artist’s Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes) and evaluation of its antiproliferative activity in HT-29 colon cancer cells
he growth and lectin production of Ganoderma applanatum, a white rot fungus, was optimized in broth cultures. The fungus was found to have a higher growth rate and higher lectin activity when grown in a medium adjusted to pH 6.5 at 26°C under stationary conditions. Expression of lectin activity star...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Begell House
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62231/1/Isolation%20and%20characterization%20of%20lectin%20from%20the%20Artist%E2%80%99s%20Conk%20medicinal%20mushroom.pdf |
| _version_ | 1796977595933261824 |
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| author | Kumaran, Sekar Pandurangan, Ashok Kumar Shenbhagaraman, Ramalingam Mohd Esa, Norhaizan |
| author_facet | Kumaran, Sekar Pandurangan, Ashok Kumar Shenbhagaraman, Ramalingam Mohd Esa, Norhaizan |
| author_sort | Kumaran, Sekar |
| collection | UPM |
| description | he growth and lectin production of Ganoderma applanatum, a white rot fungus, was optimized in broth cultures. The fungus was found to have a higher growth rate and higher lectin activity when grown in a medium adjusted to pH 6.5 at 26°C under stationary conditions. Expression of lectin activity started in 5-day-old mycelial culture; maximum activity was expressed after the 15th day of incubation. Among the various carbon and nitrogen sources tested, the carbon source sucrose and the nitrogen source yeast extract support maximum growth and lectin production. Lectin from G. applanatum was purified by ammonium sulfate precipitation and ion exchange chromatography. The purified fraction revealed a single band with a molecular weight of 35.0 kDa. Moreover, carbohydrates such as mannitol, glucose, sucrose, maltose, mannose, galactose, sorbose, and fructose were found to inhibit the hemagglutinating activity of the lectin. The purified lectins from G. applanatum contain cytotoxic and proapoptotic activities against HT-29 colon adenocarcinoma cells. |
| first_indexed | 2024-03-06T09:42:23Z |
| format | Article |
| id | upm.eprints-62231 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:42:23Z |
| publishDate | 2017 |
| publisher | Begell House |
| record_format | dspace |
| spelling | upm.eprints-622312019-05-21T04:08:42Z http://psasir.upm.edu.my/id/eprint/62231/ Isolation and characterization of lectin from the Artist’s Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes) and evaluation of its antiproliferative activity in HT-29 colon cancer cells Kumaran, Sekar Pandurangan, Ashok Kumar Shenbhagaraman, Ramalingam Mohd Esa, Norhaizan he growth and lectin production of Ganoderma applanatum, a white rot fungus, was optimized in broth cultures. The fungus was found to have a higher growth rate and higher lectin activity when grown in a medium adjusted to pH 6.5 at 26°C under stationary conditions. Expression of lectin activity started in 5-day-old mycelial culture; maximum activity was expressed after the 15th day of incubation. Among the various carbon and nitrogen sources tested, the carbon source sucrose and the nitrogen source yeast extract support maximum growth and lectin production. Lectin from G. applanatum was purified by ammonium sulfate precipitation and ion exchange chromatography. The purified fraction revealed a single band with a molecular weight of 35.0 kDa. Moreover, carbohydrates such as mannitol, glucose, sucrose, maltose, mannose, galactose, sorbose, and fructose were found to inhibit the hemagglutinating activity of the lectin. The purified lectins from G. applanatum contain cytotoxic and proapoptotic activities against HT-29 colon adenocarcinoma cells. Begell House 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62231/1/Isolation%20and%20characterization%20of%20lectin%20from%20the%20Artist%E2%80%99s%20Conk%20medicinal%20mushroom.pdf Kumaran, Sekar and Pandurangan, Ashok Kumar and Shenbhagaraman, Ramalingam and Mohd Esa, Norhaizan (2017) Isolation and characterization of lectin from the Artist’s Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes) and evaluation of its antiproliferative activity in HT-29 colon cancer cells. International Journal of Medicinal Mushrooms, 19 (8). 675 - 684. ISSN 1521-9437; ESSN: 1940-4344 http://www.dl.begellhouse.com/journals/708ae68d64b17c52,32c8651274405055,17d31937050a7002.html 10.1615/IntJMedMushrooms.2017021274 |
| spellingShingle | Kumaran, Sekar Pandurangan, Ashok Kumar Shenbhagaraman, Ramalingam Mohd Esa, Norhaizan Isolation and characterization of lectin from the Artist’s Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes) and evaluation of its antiproliferative activity in HT-29 colon cancer cells |
| title | Isolation and characterization of lectin from the Artist’s Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes) and evaluation of its antiproliferative activity in HT-29 colon cancer cells |
| title_full | Isolation and characterization of lectin from the Artist’s Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes) and evaluation of its antiproliferative activity in HT-29 colon cancer cells |
| title_fullStr | Isolation and characterization of lectin from the Artist’s Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes) and evaluation of its antiproliferative activity in HT-29 colon cancer cells |
| title_full_unstemmed | Isolation and characterization of lectin from the Artist’s Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes) and evaluation of its antiproliferative activity in HT-29 colon cancer cells |
| title_short | Isolation and characterization of lectin from the Artist’s Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes) and evaluation of its antiproliferative activity in HT-29 colon cancer cells |
| title_sort | isolation and characterization of lectin from the artist s conk medicinal mushroom ganoderma applanatum agaricomycetes and evaluation of its antiproliferative activity in ht 29 colon cancer cells |
| url | http://psasir.upm.edu.my/id/eprint/62231/1/Isolation%20and%20characterization%20of%20lectin%20from%20the%20Artist%E2%80%99s%20Conk%20medicinal%20mushroom.pdf |
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