Metabolomics study in methylotrophic yeast: a minireview
Methylotrophic yeast has been used as a cost-effective and valuable host for expression of recombinant protein due to its unique methanol utilisation pathway. It has an AOX (alcohol oxidase) protein which has been characterised to be a strong and tightly methanol-inducible dependent promoter. Metabo...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Kamla-Raj Enterprises
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62398/1/Metabolomics%20study%20in%20methylotrophic%20yeast.pdf |
| _version_ | 1796977619580747776 |
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| author | Fam, Jye Ping Sabri, Suriana Baharum, Syarul Nataqain Salleh, Abu Bakar Oslan, Siti Nurbaya |
| author_facet | Fam, Jye Ping Sabri, Suriana Baharum, Syarul Nataqain Salleh, Abu Bakar Oslan, Siti Nurbaya |
| author_sort | Fam, Jye Ping |
| collection | UPM |
| description | Methylotrophic yeast has been used as a cost-effective and valuable host for expression of recombinant protein due to its unique methanol utilisation pathway. It has an AOX (alcohol oxidase) protein which has been characterised to be a strong and tightly methanol-inducible dependent promoter. Metabolomics is the systematic study and inclusive analysis of small molecules called metabolites in a biological system. Metabolomics plays an important part in connecting the phenotype and genotype gap because it magnifies the modifications in the proteome and provides a better phenotype representation of an organism. This quantitative study has provided a new perception on the metabolic burden derived from the overexpression of recombinant protein in methylotrophic yeast. In this review, we discuss the fundamental aspect of metabolomics in methylotrophic yeast followed by their latest developments. |
| first_indexed | 2024-03-06T09:42:44Z |
| format | Article |
| id | upm.eprints-62398 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:42:44Z |
| publishDate | 2017 |
| publisher | Kamla-Raj Enterprises |
| record_format | dspace |
| spelling | upm.eprints-623982020-01-10T06:19:56Z http://psasir.upm.edu.my/id/eprint/62398/ Metabolomics study in methylotrophic yeast: a minireview Fam, Jye Ping Sabri, Suriana Baharum, Syarul Nataqain Salleh, Abu Bakar Oslan, Siti Nurbaya Methylotrophic yeast has been used as a cost-effective and valuable host for expression of recombinant protein due to its unique methanol utilisation pathway. It has an AOX (alcohol oxidase) protein which has been characterised to be a strong and tightly methanol-inducible dependent promoter. Metabolomics is the systematic study and inclusive analysis of small molecules called metabolites in a biological system. Metabolomics plays an important part in connecting the phenotype and genotype gap because it magnifies the modifications in the proteome and provides a better phenotype representation of an organism. This quantitative study has provided a new perception on the metabolic burden derived from the overexpression of recombinant protein in methylotrophic yeast. In this review, we discuss the fundamental aspect of metabolomics in methylotrophic yeast followed by their latest developments. Kamla-Raj Enterprises 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62398/1/Metabolomics%20study%20in%20methylotrophic%20yeast.pdf Fam, Jye Ping and Sabri, Suriana and Baharum, Syarul Nataqain and Salleh, Abu Bakar and Oslan, Siti Nurbaya (2017) Metabolomics study in methylotrophic yeast: a minireview. Journal of Life Sciences, 11. pp. 11-18. ISSN 1934-7391; ESSN: 1934-7405 http://www.davidpublisher.org/index.php/Home/Article/index?id=31671.html 10.17265/1934-7391/2017.01.002 |
| spellingShingle | Fam, Jye Ping Sabri, Suriana Baharum, Syarul Nataqain Salleh, Abu Bakar Oslan, Siti Nurbaya Metabolomics study in methylotrophic yeast: a minireview |
| title | Metabolomics study in methylotrophic yeast: a minireview |
| title_full | Metabolomics study in methylotrophic yeast: a minireview |
| title_fullStr | Metabolomics study in methylotrophic yeast: a minireview |
| title_full_unstemmed | Metabolomics study in methylotrophic yeast: a minireview |
| title_short | Metabolomics study in methylotrophic yeast: a minireview |
| title_sort | metabolomics study in methylotrophic yeast a minireview |
| url | http://psasir.upm.edu.my/id/eprint/62398/1/Metabolomics%20study%20in%20methylotrophic%20yeast.pdf |
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