Optimization of cultural conditions for polygalacturonase production by a newly isolated Aspergillus fumigatus R6 capable of retting kenaf
Sequential optimization strategy was used to enhance the production of extracellular polygalacturonase by a newly isolated Aspergillus fumigatus R6 using rice bran as a substrate in solid state conditions. Three significant variables influencing the polygalacturonase production were identified as in...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62610/1/R6.pdf |
| _version_ | 1825932498731794432 |
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| author | Wong, Li Yin Saad, Wan Zuhainis Mohamad, Rosfarizan Md Tahir, Paridah |
| author_facet | Wong, Li Yin Saad, Wan Zuhainis Mohamad, Rosfarizan Md Tahir, Paridah |
| author_sort | Wong, Li Yin |
| collection | UPM |
| description | Sequential optimization strategy was used to enhance the production of extracellular polygalacturonase by a newly isolated Aspergillus fumigatus R6 using rice bran as a substrate in solid state conditions. Three significant variables influencing the polygalacturonase production were identified as initial moisture level, temperature and incubation time (P < 0.0001). The model established by face-centered central composite design was significant (P < 0.05) with high R2 (0.98). The model validity was verified and the optimum conditions were at an initial moisture level of 49.6%, 33 °C and 129 h of incubation time with the maximum polygalacturonase activity of 565 U/g, resulted in 2.65 fold increase in polygalacturonase activity compare to the unoptimized conditions. Kenaf stem treated with A. fumigatus R6 polygalacturonase enzyme at 72 h produced high strength of kenaf bast fibers (287 MPa) with high Young’s modulus (10404 MPa) and the color is in satisfactory. |
| first_indexed | 2024-03-06T09:42:57Z |
| format | Article |
| id | upm.eprints-62610 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:42:57Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-626102020-12-03T20:13:29Z http://psasir.upm.edu.my/id/eprint/62610/ Optimization of cultural conditions for polygalacturonase production by a newly isolated Aspergillus fumigatus R6 capable of retting kenaf Wong, Li Yin Saad, Wan Zuhainis Mohamad, Rosfarizan Md Tahir, Paridah Sequential optimization strategy was used to enhance the production of extracellular polygalacturonase by a newly isolated Aspergillus fumigatus R6 using rice bran as a substrate in solid state conditions. Three significant variables influencing the polygalacturonase production were identified as initial moisture level, temperature and incubation time (P < 0.0001). The model established by face-centered central composite design was significant (P < 0.05) with high R2 (0.98). The model validity was verified and the optimum conditions were at an initial moisture level of 49.6%, 33 °C and 129 h of incubation time with the maximum polygalacturonase activity of 565 U/g, resulted in 2.65 fold increase in polygalacturonase activity compare to the unoptimized conditions. Kenaf stem treated with A. fumigatus R6 polygalacturonase enzyme at 72 h produced high strength of kenaf bast fibers (287 MPa) with high Young’s modulus (10404 MPa) and the color is in satisfactory. Elsevier 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62610/1/R6.pdf Wong, Li Yin and Saad, Wan Zuhainis and Mohamad, Rosfarizan and Md Tahir, Paridah (2017) Optimization of cultural conditions for polygalacturonase production by a newly isolated Aspergillus fumigatus R6 capable of retting kenaf. Industrial Crops and Products, 97. pp. 175-183. ISSN 0926-6690 https://www.sciencedirect.com/science/article/pii/S0926669016308494#! 10.1016/j.indcrop.2016.12.019 |
| spellingShingle | Wong, Li Yin Saad, Wan Zuhainis Mohamad, Rosfarizan Md Tahir, Paridah Optimization of cultural conditions for polygalacturonase production by a newly isolated Aspergillus fumigatus R6 capable of retting kenaf |
| title | Optimization of cultural conditions for polygalacturonase production by a newly isolated Aspergillus fumigatus R6 capable of retting kenaf |
| title_full | Optimization of cultural conditions for polygalacturonase production by a newly isolated Aspergillus fumigatus R6 capable of retting kenaf |
| title_fullStr | Optimization of cultural conditions for polygalacturonase production by a newly isolated Aspergillus fumigatus R6 capable of retting kenaf |
| title_full_unstemmed | Optimization of cultural conditions for polygalacturonase production by a newly isolated Aspergillus fumigatus R6 capable of retting kenaf |
| title_short | Optimization of cultural conditions for polygalacturonase production by a newly isolated Aspergillus fumigatus R6 capable of retting kenaf |
| title_sort | optimization of cultural conditions for polygalacturonase production by a newly isolated aspergillus fumigatus r6 capable of retting kenaf |
| url | http://psasir.upm.edu.my/id/eprint/62610/1/R6.pdf |
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