Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4

This study is on the optimization of the medium for solubilization of phosphate based on the Box-Behnken design and response surface methodology. Optimization of the liquid medium for phosphate solubilization using Serratia marcescens strain AGKT4 was carried out by varying the concentrations of 3 i...

Full description

Bibliographic Details
Main Authors: Abd Samad, Mohd Yusoff, Sulaiman, Zulkefly, Adekunle, Salisu Monsuru, Mohd Jaafar, Ahmad Kamil
Format: Article
Language:English
Published: Turkish Science and Technology Publishing 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62614/1/LIQUID.pdf
_version_ 1825932499117670400
author Abd Samad, Mohd Yusoff
Sulaiman, Zulkefly
Adekunle, Salisu Monsuru
Mohd Jaafar, Ahmad Kamil
author_facet Abd Samad, Mohd Yusoff
Sulaiman, Zulkefly
Adekunle, Salisu Monsuru
Mohd Jaafar, Ahmad Kamil
author_sort Abd Samad, Mohd Yusoff
collection UPM
description This study is on the optimization of the medium for solubilization of phosphate based on the Box-Behnken design and response surface methodology. Optimization of the liquid medium for phosphate solubilization using Serratia marcescens strain AGKT4 was carried out by varying the concentrations of 3 ingredients; the fructose, peptone and inoculum size of bacteria. A mathematical model derived from the response surface methodology was then validated statistically for the target test variables. The highest phosphate solubilization in the medium was achieved at the optimal concentrations of fructose and peptone at 6% (w/v) and 0.6% (w/v), respectively. The maximum phosphate solubilization at these concentrations was 239.12 µg/mL. Under the same conditions, the bacterial growth in the medium was 9 log10 CFU.
first_indexed 2024-03-06T09:42:58Z
format Article
id upm.eprints-62614
institution Universiti Putra Malaysia
language English
last_indexed 2024-03-06T09:42:58Z
publishDate 2017
publisher Turkish Science and Technology Publishing
record_format dspace
spelling upm.eprints-626142020-12-02T23:58:27Z http://psasir.upm.edu.my/id/eprint/62614/ Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4 Abd Samad, Mohd Yusoff Sulaiman, Zulkefly Adekunle, Salisu Monsuru Mohd Jaafar, Ahmad Kamil This study is on the optimization of the medium for solubilization of phosphate based on the Box-Behnken design and response surface methodology. Optimization of the liquid medium for phosphate solubilization using Serratia marcescens strain AGKT4 was carried out by varying the concentrations of 3 ingredients; the fructose, peptone and inoculum size of bacteria. A mathematical model derived from the response surface methodology was then validated statistically for the target test variables. The highest phosphate solubilization in the medium was achieved at the optimal concentrations of fructose and peptone at 6% (w/v) and 0.6% (w/v), respectively. The maximum phosphate solubilization at these concentrations was 239.12 µg/mL. Under the same conditions, the bacterial growth in the medium was 9 log10 CFU. Turkish Science and Technology Publishing 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62614/1/LIQUID.pdf Abd Samad, Mohd Yusoff and Sulaiman, Zulkefly and Adekunle, Salisu Monsuru and Mohd Jaafar, Ahmad Kamil (2017) Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4. Turkish Journal of Agriculture - Food Science and Technology, 5 (12). 1626 - 1632. ISSN 2148-127X http://www.agrifoodscience.com/index.php/TURJAF/article/view/1495 10.24925/turjaf.v5i12.1626-1632.1495
spellingShingle Abd Samad, Mohd Yusoff
Sulaiman, Zulkefly
Adekunle, Salisu Monsuru
Mohd Jaafar, Ahmad Kamil
Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4
title Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4
title_full Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4
title_fullStr Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4
title_full_unstemmed Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4
title_short Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4
title_sort optimization of liquid medium for high phosphate solubilization by serratia marcescens strain agkt4
url http://psasir.upm.edu.my/id/eprint/62614/1/LIQUID.pdf
work_keys_str_mv AT abdsamadmohdyusoff optimizationofliquidmediumforhighphosphatesolubilizationbyserratiamarcescensstrainagkt4
AT sulaimanzulkefly optimizationofliquidmediumforhighphosphatesolubilizationbyserratiamarcescensstrainagkt4
AT adekunlesalisumonsuru optimizationofliquidmediumforhighphosphatesolubilizationbyserratiamarcescensstrainagkt4
AT mohdjaafarahmadkamil optimizationofliquidmediumforhighphosphatesolubilizationbyserratiamarcescensstrainagkt4