Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4
This study is on the optimization of the medium for solubilization of phosphate based on the Box-Behnken design and response surface methodology. Optimization of the liquid medium for phosphate solubilization using Serratia marcescens strain AGKT4 was carried out by varying the concentrations of 3 i...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
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Turkish Science and Technology Publishing
2017
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Online Access: | http://psasir.upm.edu.my/id/eprint/62614/1/LIQUID.pdf |
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author | Abd Samad, Mohd Yusoff Sulaiman, Zulkefly Adekunle, Salisu Monsuru Mohd Jaafar, Ahmad Kamil |
author_facet | Abd Samad, Mohd Yusoff Sulaiman, Zulkefly Adekunle, Salisu Monsuru Mohd Jaafar, Ahmad Kamil |
author_sort | Abd Samad, Mohd Yusoff |
collection | UPM |
description | This study is on the optimization of the medium for solubilization of phosphate based on the Box-Behnken design and response surface methodology. Optimization of the liquid medium for phosphate solubilization using Serratia marcescens strain AGKT4 was carried out by varying the concentrations of 3 ingredients; the fructose, peptone and inoculum size of bacteria. A mathematical model derived from the response surface methodology was then validated statistically for the target test variables. The highest phosphate solubilization in the medium was achieved at the optimal concentrations of fructose and peptone at 6% (w/v) and 0.6% (w/v), respectively. The maximum phosphate solubilization at these concentrations was 239.12 µg/mL. Under the same conditions, the bacterial growth in the medium was 9 log10 CFU. |
first_indexed | 2024-03-06T09:42:58Z |
format | Article |
id | upm.eprints-62614 |
institution | Universiti Putra Malaysia |
language | English |
last_indexed | 2024-03-06T09:42:58Z |
publishDate | 2017 |
publisher | Turkish Science and Technology Publishing |
record_format | dspace |
spelling | upm.eprints-626142020-12-02T23:58:27Z http://psasir.upm.edu.my/id/eprint/62614/ Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4 Abd Samad, Mohd Yusoff Sulaiman, Zulkefly Adekunle, Salisu Monsuru Mohd Jaafar, Ahmad Kamil This study is on the optimization of the medium for solubilization of phosphate based on the Box-Behnken design and response surface methodology. Optimization of the liquid medium for phosphate solubilization using Serratia marcescens strain AGKT4 was carried out by varying the concentrations of 3 ingredients; the fructose, peptone and inoculum size of bacteria. A mathematical model derived from the response surface methodology was then validated statistically for the target test variables. The highest phosphate solubilization in the medium was achieved at the optimal concentrations of fructose and peptone at 6% (w/v) and 0.6% (w/v), respectively. The maximum phosphate solubilization at these concentrations was 239.12 µg/mL. Under the same conditions, the bacterial growth in the medium was 9 log10 CFU. Turkish Science and Technology Publishing 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62614/1/LIQUID.pdf Abd Samad, Mohd Yusoff and Sulaiman, Zulkefly and Adekunle, Salisu Monsuru and Mohd Jaafar, Ahmad Kamil (2017) Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4. Turkish Journal of Agriculture - Food Science and Technology, 5 (12). 1626 - 1632. ISSN 2148-127X http://www.agrifoodscience.com/index.php/TURJAF/article/view/1495 10.24925/turjaf.v5i12.1626-1632.1495 |
spellingShingle | Abd Samad, Mohd Yusoff Sulaiman, Zulkefly Adekunle, Salisu Monsuru Mohd Jaafar, Ahmad Kamil Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4 |
title | Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4 |
title_full | Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4 |
title_fullStr | Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4 |
title_full_unstemmed | Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4 |
title_short | Optimization of liquid medium for high phosphate solubilization by Serratia marcescens strain AGKT4 |
title_sort | optimization of liquid medium for high phosphate solubilization by serratia marcescens strain agkt4 |
url | http://psasir.upm.edu.my/id/eprint/62614/1/LIQUID.pdf |
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