PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis
Electrochemical biosensor holds great interest in establishing robust analytical method for Mycobacterium tuberculosis (M. Tuberculosis). Herein, fabrication of a highly sensitive electrochemical PNA biosensor based on functionalized graphene oxide (NH2-GO) composited with CdS quantum dots (QDs) for...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62772/1/PNA%20biosensor%20.pdf |
| _version_ | 1825932513188511744 |
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| author | Mat Zaid, Mohd Hazani Abdullah, Jaafar Yusof, Nor Azah Sulaiman, Yusran Wasoh, Helmi Md Noh, Mohd Fairulnizal Issa, Rahizan |
| author_facet | Mat Zaid, Mohd Hazani Abdullah, Jaafar Yusof, Nor Azah Sulaiman, Yusran Wasoh, Helmi Md Noh, Mohd Fairulnizal Issa, Rahizan |
| author_sort | Mat Zaid, Mohd Hazani |
| collection | UPM |
| description | Electrochemical biosensor holds great interest in establishing robust analytical method for Mycobacterium tuberculosis (M. Tuberculosis). Herein, fabrication of a highly sensitive electrochemical PNA biosensor based on functionalized graphene oxide (NH2-GO) composited with CdS quantum dots (QDs) for the detection of M. Tuberculosis has been described. Firstly, NH2-GO/QDs was applied onto screen-printed carbon electrode (SPCE) surface by electro-deposition method, then the PNA probe was immobilized onto the surface of NH2-GO/QDs modified SPCE via EDC/NHS coupling technique. Subsequently, the developed PNA biosensor was used to hybridize with target DNA. Differential pulse voltammetry (DPV) was employed to monitor the hybridization event by using methylene blue (MB) as the electrochemical indicator. Under the optimal conditions, a linear detection range of the PNA biosensor was obtained from 1 × 10−11 to 1 × 10−7 M with the detection limit of 8.948 × 10−13 M. The biosensor has successfully discriminated between negative and positive sample of M. Tuberculosis DNA sequences from real sample analysis. |
| first_indexed | 2024-03-06T09:43:11Z |
| format | Article |
| id | upm.eprints-62772 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:43:11Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-627722022-11-08T04:38:48Z http://psasir.upm.edu.my/id/eprint/62772/ PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis Mat Zaid, Mohd Hazani Abdullah, Jaafar Yusof, Nor Azah Sulaiman, Yusran Wasoh, Helmi Md Noh, Mohd Fairulnizal Issa, Rahizan Electrochemical biosensor holds great interest in establishing robust analytical method for Mycobacterium tuberculosis (M. Tuberculosis). Herein, fabrication of a highly sensitive electrochemical PNA biosensor based on functionalized graphene oxide (NH2-GO) composited with CdS quantum dots (QDs) for the detection of M. Tuberculosis has been described. Firstly, NH2-GO/QDs was applied onto screen-printed carbon electrode (SPCE) surface by electro-deposition method, then the PNA probe was immobilized onto the surface of NH2-GO/QDs modified SPCE via EDC/NHS coupling technique. Subsequently, the developed PNA biosensor was used to hybridize with target DNA. Differential pulse voltammetry (DPV) was employed to monitor the hybridization event by using methylene blue (MB) as the electrochemical indicator. Under the optimal conditions, a linear detection range of the PNA biosensor was obtained from 1 × 10−11 to 1 × 10−7 M with the detection limit of 8.948 × 10−13 M. The biosensor has successfully discriminated between negative and positive sample of M. Tuberculosis DNA sequences from real sample analysis. Elsevier 2017-03 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62772/1/PNA%20biosensor%20.pdf Mat Zaid, Mohd Hazani and Abdullah, Jaafar and Yusof, Nor Azah and Sulaiman, Yusran and Wasoh, Helmi and Md Noh, Mohd Fairulnizal and Issa, Rahizan (2017) PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis. Sensors and Actuators B: Chemical, 241. pp. 1024-1034. ISSN 0925-4005; ESSN: 1873-3077 https://reader.elsevier.com/reader/sd/pii/S0925400516316719?token=74F4FFAA1292459646C346F77E26CC1500395A0840F041CDDB56F30E9394D8ABDEAF70E0629A4813CEA8EE30E536913F&originRegion=eu-west-1&originCreation=20220920075007 10.1016/j.snb.2016.10.045 |
| spellingShingle | Mat Zaid, Mohd Hazani Abdullah, Jaafar Yusof, Nor Azah Sulaiman, Yusran Wasoh, Helmi Md Noh, Mohd Fairulnizal Issa, Rahizan PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis |
| title | PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis |
| title_full | PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis |
| title_fullStr | PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis |
| title_full_unstemmed | PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis |
| title_short | PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis |
| title_sort | pna biosensor based on reduced graphene oxide water soluble quantum dots for the detection of mycobacterium tuberculosis |
| url | http://psasir.upm.edu.my/id/eprint/62772/1/PNA%20biosensor%20.pdf |
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