Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells
Human pluripotent stem cells (hPSCs), including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), have the potential to differentiate into many cell types that originate from the three germ layers, such as dopamine-secreting cells and insulin-secreting cells for the treatment...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62779/1/Polymeric%20design%20of%20cell%20.pdf |
| _version_ | 1825932514352431104 |
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| author | Higuchi, Akon Subbiah, Suresh Kumar Ling, Qing-Dong A. Alarfaj, Abdullah Munusamy, Murugan A. Murugan, Kadarkarai Hsu, Shih-Tien Benelli, Giovanni Umezawa, Akihiro |
| author_facet | Higuchi, Akon Subbiah, Suresh Kumar Ling, Qing-Dong A. Alarfaj, Abdullah Munusamy, Murugan A. Murugan, Kadarkarai Hsu, Shih-Tien Benelli, Giovanni Umezawa, Akihiro |
| author_sort | Higuchi, Akon |
| collection | UPM |
| description | Human pluripotent stem cells (hPSCs), including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), have the potential to differentiate into many cell types that originate from the three germ layers, such as dopamine-secreting cells and insulin-secreting cells for the treatment of Alzheimer's disease and diabetes, respectively. However, it is challenging to guide hPSC differentiation into desired cell lineages due to their varying differentiation ability. A reasonable strategy is to mimic the stem cell microenvironment for the differentiation of hPSCs into specific cell lineages using optimal polymeric biomaterials for hPSC culture. This review summarizes various methods for differentiating hPSCs cultured on polymeric biomaterials and discusses the optimal methods and cell culture polymeric biomaterials for hPSC differentiation into specific cell lineages. The recent trend in protocols avoids embryoid body (EB, aggregated cells) formation because EBs contain different types of cells. The combination of appropriate differentiation protocols and cell culture polymeric biomaterials for the differentiation of hPSCs into specific cell lineages will produce a large quantity of highly pure GMP-grade differentiated cells for use in translational medicine. |
| first_indexed | 2024-03-06T09:43:12Z |
| format | Article |
| id | upm.eprints-62779 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:43:12Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-627792022-11-08T07:46:10Z http://psasir.upm.edu.my/id/eprint/62779/ Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells Higuchi, Akon Subbiah, Suresh Kumar Ling, Qing-Dong A. Alarfaj, Abdullah Munusamy, Murugan A. Murugan, Kadarkarai Hsu, Shih-Tien Benelli, Giovanni Umezawa, Akihiro Human pluripotent stem cells (hPSCs), including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), have the potential to differentiate into many cell types that originate from the three germ layers, such as dopamine-secreting cells and insulin-secreting cells for the treatment of Alzheimer's disease and diabetes, respectively. However, it is challenging to guide hPSC differentiation into desired cell lineages due to their varying differentiation ability. A reasonable strategy is to mimic the stem cell microenvironment for the differentiation of hPSCs into specific cell lineages using optimal polymeric biomaterials for hPSC culture. This review summarizes various methods for differentiating hPSCs cultured on polymeric biomaterials and discusses the optimal methods and cell culture polymeric biomaterials for hPSC differentiation into specific cell lineages. The recent trend in protocols avoids embryoid body (EB, aggregated cells) formation because EBs contain different types of cells. The combination of appropriate differentiation protocols and cell culture polymeric biomaterials for the differentiation of hPSCs into specific cell lineages will produce a large quantity of highly pure GMP-grade differentiated cells for use in translational medicine. Elsevier 2017-02 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62779/1/Polymeric%20design%20of%20cell%20.pdf Higuchi, Akon and Subbiah, Suresh Kumar and Ling, Qing-Dong and A. Alarfaj, Abdullah and Munusamy, Murugan A. and Murugan, Kadarkarai and Hsu, Shih-Tien and Benelli, Giovanni and Umezawa, Akihiro (2017) Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells. Progress in Polymer Science, 65. 83 - 126. ISSN 0079-6700 https://reader.elsevier.com/reader/sd/pii/S007967001630065X?token=59ECB83AC389531DB3F3256CB96A7493C4C0C37826285ADECE0C39DA8C03118FE1AC67A1859639D7E38CBA878D968354&originRegion=eu-west-1&originCreation=20220921073042 10.1016/j.progpolymsci.2016.09.002 |
| spellingShingle | Higuchi, Akon Subbiah, Suresh Kumar Ling, Qing-Dong A. Alarfaj, Abdullah Munusamy, Murugan A. Murugan, Kadarkarai Hsu, Shih-Tien Benelli, Giovanni Umezawa, Akihiro Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells |
| title | Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells |
| title_full | Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells |
| title_fullStr | Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells |
| title_full_unstemmed | Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells |
| title_short | Polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells |
| title_sort | polymeric design of cell culture materials that guide the differentiation of human pluripotent stem cells |
| url | http://psasir.upm.edu.my/id/eprint/62779/1/Polymeric%20design%20of%20cell%20.pdf |
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