Population structure of the blue swimmer crab Portunus pelagicus in coastal areas of Malaysia inferred from microsatellites
Portunus pelagicus, distributed throughout the Indo-West Pacific region, is one of the large and edible species of blue swimmer crabs. Increasing demand for the frozen and canned crabmeat industry worldwide has now relied mainly on P. pelagicus which in turn generates splendid income for the fisherm...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Academia Sinica
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62781/1/Population%20structure%20of%20the%20blue%20swimmer%20.pdf |
| _version_ | 1825932514742501376 |
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| author | Chuan, Jian Chai Esa, Yuzine Ismail, Muhammad Fadhil Syukri Kamarudin, Mohd. Salleh |
| author_facet | Chuan, Jian Chai Esa, Yuzine Ismail, Muhammad Fadhil Syukri Kamarudin, Mohd. Salleh |
| author_sort | Chuan, Jian Chai |
| collection | UPM |
| description | Portunus pelagicus, distributed throughout the Indo-West Pacific region, is one of the large and edible species of blue swimmer crabs. Increasing demand for the frozen and canned crabmeat industry worldwide has now relied mainly on P. pelagicus which in turn generates splendid income for the fisherman communities. In the present study, the population genetic structure of P. pelagicus was examined using six pairs of microsatellite loci. A total of 87 crab samples were collected from five different coastal areas of Malaysia. Genomic DNA was extracted from each sample for polymerase chain reaction (PCR) amplification and fragment analysis. Four out of six microsatellite primers revealed polymorphic loci in P. pelagicus sampled. The number of alleles per locus in P. pelagicus ranged from 14 to 34. Microsatellites analyses indicated low levels of genetic differentiation among the P. pelagicus populations. The average observed heterozygosity (HO = 0.48) obtained was lower than the standard heterozygosity found in most marine populations (H O = 0.79). The high F IS values (mean F IS = 0.4756) and low F ST values (mean F ST = 0.0413) also suggested the existence of inbreeding among different populations of P. pelagicus. In conclusion, this study was able to shed light on the population structure of P. pelagicus in coastal areas of Malaysia. |
| first_indexed | 2024-03-06T09:43:12Z |
| format | Article |
| id | upm.eprints-62781 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:43:12Z |
| publishDate | 2017 |
| publisher | Academia Sinica |
| record_format | dspace |
| spelling | upm.eprints-627812022-11-09T00:37:48Z http://psasir.upm.edu.my/id/eprint/62781/ Population structure of the blue swimmer crab Portunus pelagicus in coastal areas of Malaysia inferred from microsatellites Chuan, Jian Chai Esa, Yuzine Ismail, Muhammad Fadhil Syukri Kamarudin, Mohd. Salleh Portunus pelagicus, distributed throughout the Indo-West Pacific region, is one of the large and edible species of blue swimmer crabs. Increasing demand for the frozen and canned crabmeat industry worldwide has now relied mainly on P. pelagicus which in turn generates splendid income for the fisherman communities. In the present study, the population genetic structure of P. pelagicus was examined using six pairs of microsatellite loci. A total of 87 crab samples were collected from five different coastal areas of Malaysia. Genomic DNA was extracted from each sample for polymerase chain reaction (PCR) amplification and fragment analysis. Four out of six microsatellite primers revealed polymorphic loci in P. pelagicus sampled. The number of alleles per locus in P. pelagicus ranged from 14 to 34. Microsatellites analyses indicated low levels of genetic differentiation among the P. pelagicus populations. The average observed heterozygosity (HO = 0.48) obtained was lower than the standard heterozygosity found in most marine populations (H O = 0.79). The high F IS values (mean F IS = 0.4756) and low F ST values (mean F ST = 0.0413) also suggested the existence of inbreeding among different populations of P. pelagicus. In conclusion, this study was able to shed light on the population structure of P. pelagicus in coastal areas of Malaysia. Academia Sinica 2017-10 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62781/1/Population%20structure%20of%20the%20blue%20swimmer%20.pdf Chuan, Jian Chai and Esa, Yuzine and Ismail, Muhammad Fadhil Syukri and Kamarudin, Mohd. Salleh (2017) Population structure of the blue swimmer crab Portunus pelagicus in coastal areas of Malaysia inferred from microsatellites. Zoological Studies, 56 (26). pp. 1-12. ISSN 1810-522X https://zoolstud.sinica.edu.tw/Journals/56/56-26.html 10.6620/ZS.2017.56-26 |
| spellingShingle | Chuan, Jian Chai Esa, Yuzine Ismail, Muhammad Fadhil Syukri Kamarudin, Mohd. Salleh Population structure of the blue swimmer crab Portunus pelagicus in coastal areas of Malaysia inferred from microsatellites |
| title | Population structure of the blue swimmer crab Portunus pelagicus in coastal areas of Malaysia inferred from microsatellites |
| title_full | Population structure of the blue swimmer crab Portunus pelagicus in coastal areas of Malaysia inferred from microsatellites |
| title_fullStr | Population structure of the blue swimmer crab Portunus pelagicus in coastal areas of Malaysia inferred from microsatellites |
| title_full_unstemmed | Population structure of the blue swimmer crab Portunus pelagicus in coastal areas of Malaysia inferred from microsatellites |
| title_short | Population structure of the blue swimmer crab Portunus pelagicus in coastal areas of Malaysia inferred from microsatellites |
| title_sort | population structure of the blue swimmer crab portunus pelagicus in coastal areas of malaysia inferred from microsatellites |
| url | http://psasir.upm.edu.my/id/eprint/62781/1/Population%20structure%20of%20the%20blue%20swimmer%20.pdf |
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