Preparation and characterisation of Cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane
An industrial enzyme, Cyclodextrin glucanotransferase (CGTase), was immobilised in polyvinyl alcohol (PVA) nanofiber (average diameter around 200 nm) membrane via co-electrospinning of the CGTase/PVA mixture followed with glutaraldehyde vapour phase cross-linking. Addition of enzyme with concentrati...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sen'i Gakkai,Society of Fiber Science and Technology, Japan
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62822/1/Preparation%20and%20characterisation%20.pdf |
| _version_ | 1796977657604210688 |
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| author | Saallah, Suryani Naim, Mohd Nazli Mokhtar, Mohd Noriznan Abu Bakar, Noor Fitrah Gen, Masao Lenggoro, I. Wuled |
| author_facet | Saallah, Suryani Naim, Mohd Nazli Mokhtar, Mohd Noriznan Abu Bakar, Noor Fitrah Gen, Masao Lenggoro, I. Wuled |
| author_sort | Saallah, Suryani |
| collection | UPM |
| description | An industrial enzyme, Cyclodextrin glucanotransferase (CGTase), was immobilised in polyvinyl alcohol (PVA) nanofiber (average diameter around 200 nm) membrane via co-electrospinning of the CGTase/PVA mixture followed with glutaraldehyde vapour phase cross-linking. Addition of enzyme with concentration ranging from 1.5 to 7.5 % to the PVA solution (8 wt%) caused significant changes to the liquid jet behaviours which consequently affected the nanofiber structures and sizes. Incorporation of CGTase in the PVA membrane was confirmed by Raman spectroscopic analysis. The Raman spectra also showed no structural changes occured to the enzyme after subjected to the electrostatic spinning and cross-linking reaction. The immobilised enzyme showed excellent catalytic efficiency with up to 3.6 times higher enzyme loading, 25 % higher activity and good reusability in comparison with CGTase/PVA film made up from the same starting solution (control). |
| first_indexed | 2024-03-06T09:43:18Z |
| format | Article |
| id | upm.eprints-62822 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:43:18Z |
| publishDate | 2017 |
| publisher | Sen'i Gakkai,Society of Fiber Science and Technology, Japan |
| record_format | dspace |
| spelling | upm.eprints-628222022-11-10T01:40:06Z http://psasir.upm.edu.my/id/eprint/62822/ Preparation and characterisation of Cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane Saallah, Suryani Naim, Mohd Nazli Mokhtar, Mohd Noriznan Abu Bakar, Noor Fitrah Gen, Masao Lenggoro, I. Wuled An industrial enzyme, Cyclodextrin glucanotransferase (CGTase), was immobilised in polyvinyl alcohol (PVA) nanofiber (average diameter around 200 nm) membrane via co-electrospinning of the CGTase/PVA mixture followed with glutaraldehyde vapour phase cross-linking. Addition of enzyme with concentration ranging from 1.5 to 7.5 % to the PVA solution (8 wt%) caused significant changes to the liquid jet behaviours which consequently affected the nanofiber structures and sizes. Incorporation of CGTase in the PVA membrane was confirmed by Raman spectroscopic analysis. The Raman spectra also showed no structural changes occured to the enzyme after subjected to the electrostatic spinning and cross-linking reaction. The immobilised enzyme showed excellent catalytic efficiency with up to 3.6 times higher enzyme loading, 25 % higher activity and good reusability in comparison with CGTase/PVA film made up from the same starting solution (control). Sen'i Gakkai,Society of Fiber Science and Technology, Japan 2017-10 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62822/1/Preparation%20and%20characterisation%20.pdf Saallah, Suryani and Naim, Mohd Nazli and Mokhtar, Mohd Noriznan and Abu Bakar, Noor Fitrah and Gen, Masao and Lenggoro, I. Wuled (2017) Preparation and characterisation of Cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane. Journal of Fiber Science and Technology, 73 (10). 251 - 260. ISSN 2189-7654 https://www.jstage.jst.go.jp/article/fiberst/73/10/73_2017-0035/_pdf/-char/en 10.2115/fiberst.2017-0035 |
| spellingShingle | Saallah, Suryani Naim, Mohd Nazli Mokhtar, Mohd Noriznan Abu Bakar, Noor Fitrah Gen, Masao Lenggoro, I. Wuled Preparation and characterisation of Cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane |
| title | Preparation and characterisation of Cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane |
| title_full | Preparation and characterisation of Cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane |
| title_fullStr | Preparation and characterisation of Cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane |
| title_full_unstemmed | Preparation and characterisation of Cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane |
| title_short | Preparation and characterisation of Cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane |
| title_sort | preparation and characterisation of cyclodextrin glucanotransferase enzyme immobilised in electrospun nanofibrous membrane |
| url | http://psasir.upm.edu.my/id/eprint/62822/1/Preparation%20and%20characterisation%20.pdf |
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