Pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk

Deep eutectic solvents (DES) were utilised as pretreatment media on oil palm trunk (OPT) fibre in order to change the morphology of the highly crystalline cellulose prior to enzymatic hydrolysis. Among all the DESs tested, ethylammonium chloride: ethylene glycol (EAC:EG) was found to be the most eff...

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Main Authors: Zulkefli, Syarilaida, Abdul Malek, Emilia, Abdul Rahman, Mohd Basyaruddin
Format: Article
Language:English
Published: Elsevier 2017
Online Access:http://psasir.upm.edu.my/id/eprint/62837/1/Pretreatment%20of%20oil%20palm%20trunk%20.pdf
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author Zulkefli, Syarilaida
Abdul Malek, Emilia
Abdul Rahman, Mohd Basyaruddin
author_facet Zulkefli, Syarilaida
Abdul Malek, Emilia
Abdul Rahman, Mohd Basyaruddin
author_sort Zulkefli, Syarilaida
collection UPM
description Deep eutectic solvents (DES) were utilised as pretreatment media on oil palm trunk (OPT) fibre in order to change the morphology of the highly crystalline cellulose prior to enzymatic hydrolysis. Among all the DESs tested, ethylammonium chloride: ethylene glycol (EAC:EG) was found to be the most efficient solvent for the pretreatment of OPT fibre. The pretreatment of OPT by EAC:EG had removed 42% lignin and 83% hemicellulose with the ability to dissolve the OPT biomass (58%) after heating at 100 °C for 48 h. FTIR analysis was used in determining the chemical characteristic changes where OPT treated in EAC:EG had more disruption of hydroxyl bond and indication of delignification compared to OPT treated with other DESs. Enzymatic hydrolysis was carried out on OPT treated in EAC:EG and the highest glucose production (74%) was achieved at 50 °C after 24 h with 15 mg/mL substrate concentration, 50 FPU/g of Celluclast 1.5 L and 100 CBU/ml of Novozyme 188.
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spelling upm.eprints-628372022-11-10T07:26:25Z http://psasir.upm.edu.my/id/eprint/62837/ Pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk Zulkefli, Syarilaida Abdul Malek, Emilia Abdul Rahman, Mohd Basyaruddin Deep eutectic solvents (DES) were utilised as pretreatment media on oil palm trunk (OPT) fibre in order to change the morphology of the highly crystalline cellulose prior to enzymatic hydrolysis. Among all the DESs tested, ethylammonium chloride: ethylene glycol (EAC:EG) was found to be the most efficient solvent for the pretreatment of OPT fibre. The pretreatment of OPT by EAC:EG had removed 42% lignin and 83% hemicellulose with the ability to dissolve the OPT biomass (58%) after heating at 100 °C for 48 h. FTIR analysis was used in determining the chemical characteristic changes where OPT treated in EAC:EG had more disruption of hydroxyl bond and indication of delignification compared to OPT treated with other DESs. Enzymatic hydrolysis was carried out on OPT treated in EAC:EG and the highest glucose production (74%) was achieved at 50 °C after 24 h with 15 mg/mL substrate concentration, 50 FPU/g of Celluclast 1.5 L and 100 CBU/ml of Novozyme 188. Elsevier 2017-07 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62837/1/Pretreatment%20of%20oil%20palm%20trunk%20.pdf Zulkefli, Syarilaida and Abdul Malek, Emilia and Abdul Rahman, Mohd Basyaruddin (2017) Pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk. Renewable Energy, 107. 36 - 41. ISSN 0960-1481; ESSN: 1879-0682 https://www.sciencedirect.com/science/article/pii/S0960148117300472?via%3Dihub 10.1016/j.renene.2017.01.037
spellingShingle Zulkefli, Syarilaida
Abdul Malek, Emilia
Abdul Rahman, Mohd Basyaruddin
Pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk
title Pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk
title_full Pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk
title_fullStr Pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk
title_full_unstemmed Pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk
title_short Pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk
title_sort pretreatment of oil palm trunk in deep eutectic solvent and optimization of enzymatic hydrolysis of pretreated oil palm trunk
url http://psasir.upm.edu.my/id/eprint/62837/1/Pretreatment%20of%20oil%20palm%20trunk%20.pdf
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