Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia
Campylobacter is a major foodborne pathogen frequently associated with human bacterial gastroenteritis in the world. This study was conducted to determine the prevalence and antibiotic resistance of Campylobacter spp. in the beef food system in Malaysia. A total of 340 samples consisting of cattle f...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Research Foundation
2017
|
| Online Access: | http://psasir.upm.edu.my/id/eprint/62838/1/Prevalence%20and%20antibiotic%20resistance%20.pdf |
| _version_ | 1796977660007546880 |
|---|---|
| author | Premarathne, Jayasekara M. K. J. K. Anuar, Aimi Syazana Thung, Tze Young Satharasinghe, Dilan Amila Jambari, Nuzul Noorahya Abdul Mutalib, Noor Azira Tang, John Yew Huat Basri, Dayang Fredalina Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_facet | Premarathne, Jayasekara M. K. J. K. Anuar, Aimi Syazana Thung, Tze Young Satharasinghe, Dilan Amila Jambari, Nuzul Noorahya Abdul Mutalib, Noor Azira Tang, John Yew Huat Basri, Dayang Fredalina Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_sort | Premarathne, Jayasekara M. K. J. K. |
| collection | UPM |
| description | Campylobacter is a major foodborne pathogen frequently associated with human bacterial gastroenteritis in the world. This study was conducted to determine the prevalence and antibiotic resistance of Campylobacter spp. in the beef food system in Malaysia. A total of 340 samples consisting of cattle feces (n = 100), beef (n = 120) from wet markets and beef (n = 120) from hypermarkets were analyzed for Campylobacter spp. The overall prevalence of Campylobacter was 17.4%, consisting of 33% in cattle fecal samples, 14.2% in raw beef from wet market and 7.5% in raw beef from the hypermarket. The multiplex-polymerase chain reaction (PCR) identified 55% of the strains as C. jejuni, 26% as C. coli, and 19% as other Campylobacter spp. A high percentage of Campylobacter spp. were resistant to tetracycline (76.9%) and ampicillin (69.2%), whilst low resistance was exhibited to chloramphenicol (7.6%). The MAR Index of Campylobacter isolates from this study ranged from 0.09 to 0.73. The present study indicates the potential public health risk associated with the beef food system, hence stringent surveillance, regulatory measures, and appropriate interventions are required to minimize Campylobacter contamination and prudent antibiotic usage that can ensure consumer safety. |
| first_indexed | 2024-03-06T09:43:20Z |
| format | Article |
| id | upm.eprints-62838 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:43:20Z |
| publishDate | 2017 |
| publisher | Frontiers Research Foundation |
| record_format | dspace |
| spelling | upm.eprints-628382022-11-21T00:16:17Z http://psasir.upm.edu.my/id/eprint/62838/ Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia Premarathne, Jayasekara M. K. J. K. Anuar, Aimi Syazana Thung, Tze Young Satharasinghe, Dilan Amila Jambari, Nuzul Noorahya Abdul Mutalib, Noor Azira Tang, John Yew Huat Basri, Dayang Fredalina Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Campylobacter is a major foodborne pathogen frequently associated with human bacterial gastroenteritis in the world. This study was conducted to determine the prevalence and antibiotic resistance of Campylobacter spp. in the beef food system in Malaysia. A total of 340 samples consisting of cattle feces (n = 100), beef (n = 120) from wet markets and beef (n = 120) from hypermarkets were analyzed for Campylobacter spp. The overall prevalence of Campylobacter was 17.4%, consisting of 33% in cattle fecal samples, 14.2% in raw beef from wet market and 7.5% in raw beef from the hypermarket. The multiplex-polymerase chain reaction (PCR) identified 55% of the strains as C. jejuni, 26% as C. coli, and 19% as other Campylobacter spp. A high percentage of Campylobacter spp. were resistant to tetracycline (76.9%) and ampicillin (69.2%), whilst low resistance was exhibited to chloramphenicol (7.6%). The MAR Index of Campylobacter isolates from this study ranged from 0.09 to 0.73. The present study indicates the potential public health risk associated with the beef food system, hence stringent surveillance, regulatory measures, and appropriate interventions are required to minimize Campylobacter contamination and prudent antibiotic usage that can ensure consumer safety. Frontiers Research Foundation 2017-12 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62838/1/Prevalence%20and%20antibiotic%20resistance%20.pdf Premarathne, Jayasekara M. K. J. K. and Anuar, Aimi Syazana and Thung, Tze Young and Satharasinghe, Dilan Amila and Jambari, Nuzul Noorahya and Abdul Mutalib, Noor Azira and Tang, John Yew Huat and Basri, Dayang Fredalina and Rukayadi, Yaya and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2017) Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia. Frontiers in Microbiology, 8. art. no. 2254. pp. 1-9. ISSN 1664-302X https://www.frontiersin.org/articles/10.3389/fmicb.2017.02254/full 10.3389/fmicb.2017.02254 |
| spellingShingle | Premarathne, Jayasekara M. K. J. K. Anuar, Aimi Syazana Thung, Tze Young Satharasinghe, Dilan Amila Jambari, Nuzul Noorahya Abdul Mutalib, Noor Azira Tang, John Yew Huat Basri, Dayang Fredalina Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia |
| title | Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia |
| title_full | Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia |
| title_fullStr | Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia |
| title_full_unstemmed | Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia |
| title_short | Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia |
| title_sort | prevalence and antibiotic resistance against tetracycline in campylobacter jejuni and c coli in cattle and beef meat from selangor malaysia |
| url | http://psasir.upm.edu.my/id/eprint/62838/1/Prevalence%20and%20antibiotic%20resistance%20.pdf |
| work_keys_str_mv | AT premarathnejayasekaramkjk prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia AT anuaraimisyazana prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia AT thungtzeyoung prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia AT satharasinghedilanamila prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia AT jambarinuzulnoorahya prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia AT abdulmutalibnoorazira prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia AT tangjohnyewhuat prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia AT basridayangfredalina prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia AT rukayadiyaya prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia AT nakaguchiyoshitsugu prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia AT nishibuchimitsuaki prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia AT raduson prevalenceandantibioticresistanceagainsttetracyclineincampylobacterjejuniandccoliincattleandbeefmeatfromselangormalaysia |