Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from short mackerels (Rastrelliger brachysoma) in Malaysia
Numerous prevalence studies and outbreaks of Vibrio parahaemolyticus infection have been extensively reported in shellfish and crustaceans. Information on the quantitative detection of V. parahaemolyticus in finfish species is limited. In this study, short mackerels (Rastrelliger brachysoma) obtaine...
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| Format: | Article |
| Language: | English |
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Frontiers Research Foundation
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/62839/1/Prevalence%20and%20antimicrobial%20susceptibility%20.pdf |
| _version_ | 1825932523352358912 |
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| author | Tan, Chia Wanq Tan, Malcolm Turk Hsern Kuan, Chee-Hao Thung, Tze-Young Chang, Wei-San Loo, Yuet-Ying Premarathne, Jayasekara M K J K Ramzi, Othman B. Mohd Fadzil, Siti Norshafawatie Nordin, Yusralimuna Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_facet | Tan, Chia Wanq Tan, Malcolm Turk Hsern Kuan, Chee-Hao Thung, Tze-Young Chang, Wei-San Loo, Yuet-Ying Premarathne, Jayasekara M K J K Ramzi, Othman B. Mohd Fadzil, Siti Norshafawatie Nordin, Yusralimuna Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son |
| author_sort | Tan, Chia Wanq |
| collection | UPM |
| description | Numerous prevalence studies and outbreaks of Vibrio parahaemolyticus infection have been extensively reported in shellfish and crustaceans. Information on the quantitative detection of V. parahaemolyticus in finfish species is limited. In this study, short mackerels (Rastrelliger brachysoma) obtained from different retail marketplaces were monitored with the presence of total and pathogenic strains of V. parahaemolyticus. Out of 130 short mackerel samples, 116 (89.2%) were detected with the presence of total V. parahaemolyticus and microbial loads of total V. parahaemolyticus ranging from <3 to >105 MPN/g. Prevalence of total V. parahaemolyticus was found highest in wet markets (95.2%) followed by minimarkets (89.1%) and hypermarkets (83.3%). Pathogenic V. parahaemolyticus strains (tdh+ and/or trh+) were detected in 16.2% (21 of 130) of short mackerel samples. The density of tdh+ V. parahaemolyticus strains were examined ranging from 3.6 to >105 MPN/g and microbial loads of V. parahaemolyticus strains positive for both tdh and trh were found ranging from 300 to 740 MPN/g. On the other hand, antibiotic susceptibility profiles of V. parahaemolyticus strains isolated from short mackerels were determined through disc diffusion method in this study. Assessment of antimicrobial susceptibility profile of V. parahaemolyticus revealed majority of the isolates were highly susceptible to ampicillin sulbactam, meropenem, ceftazidime, and imipenem, but resistant to penicillin G and ampicillin. Two isolates (2.99%) exhibited the highest multiple antibiotic resistance (MAR) index value of 0.41 which shown resistance to 7 antibiotics. Results of the present study demonstrated that the occurrence of pathogenic V. parahaemolyticus strains in short mackerels and multidrug resistance of V. parahaemolyticus isolates could be a potential public health concerns to the consumer. Furthermore, prevalence data attained from the current study can be further used to develop a microbial risk assessment model to estimate health risks associated with the consumption of short mackerels contaminated with pathogenic V. parahaemolyticus. |
| first_indexed | 2024-03-06T09:43:20Z |
| format | Article |
| id | upm.eprints-62839 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:43:20Z |
| publishDate | 2017 |
| publisher | Frontiers Research Foundation |
| record_format | dspace |
| spelling | upm.eprints-628392022-11-21T01:25:26Z http://psasir.upm.edu.my/id/eprint/62839/ Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from short mackerels (Rastrelliger brachysoma) in Malaysia Tan, Chia Wanq Tan, Malcolm Turk Hsern Kuan, Chee-Hao Thung, Tze-Young Chang, Wei-San Loo, Yuet-Ying Premarathne, Jayasekara M K J K Ramzi, Othman B. Mohd Fadzil, Siti Norshafawatie Nordin, Yusralimuna Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Numerous prevalence studies and outbreaks of Vibrio parahaemolyticus infection have been extensively reported in shellfish and crustaceans. Information on the quantitative detection of V. parahaemolyticus in finfish species is limited. In this study, short mackerels (Rastrelliger brachysoma) obtained from different retail marketplaces were monitored with the presence of total and pathogenic strains of V. parahaemolyticus. Out of 130 short mackerel samples, 116 (89.2%) were detected with the presence of total V. parahaemolyticus and microbial loads of total V. parahaemolyticus ranging from <3 to >105 MPN/g. Prevalence of total V. parahaemolyticus was found highest in wet markets (95.2%) followed by minimarkets (89.1%) and hypermarkets (83.3%). Pathogenic V. parahaemolyticus strains (tdh+ and/or trh+) were detected in 16.2% (21 of 130) of short mackerel samples. The density of tdh+ V. parahaemolyticus strains were examined ranging from 3.6 to >105 MPN/g and microbial loads of V. parahaemolyticus strains positive for both tdh and trh were found ranging from 300 to 740 MPN/g. On the other hand, antibiotic susceptibility profiles of V. parahaemolyticus strains isolated from short mackerels were determined through disc diffusion method in this study. Assessment of antimicrobial susceptibility profile of V. parahaemolyticus revealed majority of the isolates were highly susceptible to ampicillin sulbactam, meropenem, ceftazidime, and imipenem, but resistant to penicillin G and ampicillin. Two isolates (2.99%) exhibited the highest multiple antibiotic resistance (MAR) index value of 0.41 which shown resistance to 7 antibiotics. Results of the present study demonstrated that the occurrence of pathogenic V. parahaemolyticus strains in short mackerels and multidrug resistance of V. parahaemolyticus isolates could be a potential public health concerns to the consumer. Furthermore, prevalence data attained from the current study can be further used to develop a microbial risk assessment model to estimate health risks associated with the consumption of short mackerels contaminated with pathogenic V. parahaemolyticus. Frontiers Research Foundation 2017-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/62839/1/Prevalence%20and%20antimicrobial%20susceptibility%20.pdf Tan, Chia Wanq and Tan, Malcolm Turk Hsern and Kuan, Chee-Hao and Thung, Tze-Young and Chang, Wei-San and Loo, Yuet-Ying and Premarathne, Jayasekara M K J K and Ramzi, Othman B. and Mohd Fadzil, Siti Norshafawatie and Nordin, Yusralimuna and Rukayadi, Yaya and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2017) Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from short mackerels (Rastrelliger brachysoma) in Malaysia. Frontiers in Microbiology, 8. art. no. 1087. pp. 1-9. ISSN 1664-302X https://www.frontiersin.org/articles/10.3389/fmicb.2017.01087/full 10.3389/fmicb.2017.01087 |
| spellingShingle | Tan, Chia Wanq Tan, Malcolm Turk Hsern Kuan, Chee-Hao Thung, Tze-Young Chang, Wei-San Loo, Yuet-Ying Premarathne, Jayasekara M K J K Ramzi, Othman B. Mohd Fadzil, Siti Norshafawatie Nordin, Yusralimuna Rukayadi, Yaya Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from short mackerels (Rastrelliger brachysoma) in Malaysia |
| title | Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from short mackerels (Rastrelliger brachysoma) in Malaysia |
| title_full | Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from short mackerels (Rastrelliger brachysoma) in Malaysia |
| title_fullStr | Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from short mackerels (Rastrelliger brachysoma) in Malaysia |
| title_full_unstemmed | Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from short mackerels (Rastrelliger brachysoma) in Malaysia |
| title_short | Prevalence and antimicrobial susceptibility of Vibrio parahaemolyticus isolated from short mackerels (Rastrelliger brachysoma) in Malaysia |
| title_sort | prevalence and antimicrobial susceptibility of vibrio parahaemolyticus isolated from short mackerels rastrelliger brachysoma in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/62839/1/Prevalence%20and%20antimicrobial%20susceptibility%20.pdf |
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