Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods
Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) co...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
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Nature Publishing
2017
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| author | Lin, Hong Reng Heish, Chao-Wen Liu, Cheng-Hui Muduli, Saradaprasan Li, Hsing-Fen Higuchi, Akon Subbiah, Suresh Kumar Alarfaj, Abdullah A Munusamy, Murugan A Hsu, Shih-Tien Chen, Da-Chung Benelli, Giovanni Murugan, Kadarkarai Cheng, Nai-Chen Wang, Han-Chow Wu, Gwo-Jang |
| author_facet | Lin, Hong Reng Heish, Chao-Wen Liu, Cheng-Hui Muduli, Saradaprasan Li, Hsing-Fen Higuchi, Akon Subbiah, Suresh Kumar Alarfaj, Abdullah A Munusamy, Murugan A Hsu, Shih-Tien Chen, Da-Chung Benelli, Giovanni Murugan, Kadarkarai Cheng, Nai-Chen Wang, Han-Chow Wu, Gwo-Jang |
| author_sort | Lin, Hong Reng |
| collection | UPM |
| description | Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes. Expression of mesenchymal stem cell markers and pluripotency genes, and osteogenic differentiation were compared for hADSCs isolated by different methods using nylon mesh filter membranes with pore sizes ranging from 11 to 80 μm. hADSCs isolated by the membrane migration method had the highest MSC surface marker expression and efficient differentiation into osteoblasts. Osteogenic differentiation ability of hADSCs and MSC surface marker expression were correlated, but osteogenic differentiation ability and pluripotent gene expression were not. |
| first_indexed | 2024-03-06T09:43:27Z |
| format | Article |
| id | upm.eprints-62892 |
| institution | Universiti Putra Malaysia |
| last_indexed | 2024-03-06T09:43:27Z |
| publishDate | 2017 |
| publisher | Nature Publishing |
| record_format | dspace |
| spelling | upm.eprints-628922022-11-24T01:57:32Z http://psasir.upm.edu.my/id/eprint/62892/ Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods Lin, Hong Reng Heish, Chao-Wen Liu, Cheng-Hui Muduli, Saradaprasan Li, Hsing-Fen Higuchi, Akon Subbiah, Suresh Kumar Alarfaj, Abdullah A Munusamy, Murugan A Hsu, Shih-Tien Chen, Da-Chung Benelli, Giovanni Murugan, Kadarkarai Cheng, Nai-Chen Wang, Han-Chow Wu, Gwo-Jang Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes. Expression of mesenchymal stem cell markers and pluripotency genes, and osteogenic differentiation were compared for hADSCs isolated by different methods using nylon mesh filter membranes with pore sizes ranging from 11 to 80 μm. hADSCs isolated by the membrane migration method had the highest MSC surface marker expression and efficient differentiation into osteoblasts. Osteogenic differentiation ability of hADSCs and MSC surface marker expression were correlated, but osteogenic differentiation ability and pluripotent gene expression were not. Nature Publishing 2017-01 Article PeerReviewed Lin, Hong Reng and Heish, Chao-Wen and Liu, Cheng-Hui and Muduli, Saradaprasan and Li, Hsing-Fen and Higuchi, Akon and Subbiah, Suresh Kumar and Alarfaj, Abdullah A and Munusamy, Murugan A and Hsu, Shih-Tien and Chen, Da-Chung and Benelli, Giovanni and Murugan, Kadarkarai and Cheng, Nai-Chen and Wang, Han-Chow and Wu, Gwo-Jang (2017) Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods. Scientific Reports, 7 (1). art. no. 40069. pp. 1-13. ISSN 2045-2322 https://www.nature.com/articles/srep40069 10.1038/srep40069 |
| spellingShingle | Lin, Hong Reng Heish, Chao-Wen Liu, Cheng-Hui Muduli, Saradaprasan Li, Hsing-Fen Higuchi, Akon Subbiah, Suresh Kumar Alarfaj, Abdullah A Munusamy, Murugan A Hsu, Shih-Tien Chen, Da-Chung Benelli, Giovanni Murugan, Kadarkarai Cheng, Nai-Chen Wang, Han-Chow Wu, Gwo-Jang Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods |
| title | Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods |
| title_full | Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods |
| title_fullStr | Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods |
| title_full_unstemmed | Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods |
| title_short | Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods |
| title_sort | purification and differentiation of human adipose derived stem cells by membrane filtration and membrane migration methods |
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