Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester
BACKGROUND: The synthesis of betulinic acid ester using betulinic acid and oleyl alcohol catalyzed by Novozym 435 (immobilized Candida antarctica lipase) was carried out. Response surface methodology (RSM) based on a five-level, three-variable, central composite rotatable design (CCRD) was employe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Wiley Blackwell
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/6325/1/Response%20surface%20methodology%20as%20a%20tool%20to%20study%20the%20lipase.pdf |
| _version_ | 1796967142094012416 |
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| author | Yasin, Yamin Basri, Mahiran Ahmad, Faujan Salleh, Abu Bakar |
| author_facet | Yasin, Yamin Basri, Mahiran Ahmad, Faujan Salleh, Abu Bakar |
| author_sort | Yasin, Yamin |
| collection | UPM |
| description | BACKGROUND: The synthesis of betulinic acid ester using betulinic acid and oleyl alcohol catalyzed by Novozym
435 (immobilized Candida antarctica lipase) was carried out. Response surface methodology (RSM) based on a
five-level, three-variable, central composite rotatable design (CCRD) was employed to evaluate the interactive
effects of various parameters. The parameters were reaction time (8–16 h), temperature (20–60 ◦C) and enzyme
amount (120–160 mg).
RESULTS: Simultaneously increasing reaction time, temperature and amount of enzyme increased the yields of
betulinic acid ester produced.
CONCLUSION: The optimum conditions derived via RSM for the reaction were reaction time of 10.2 h,
temperature of 53.1 ◦C and enzyme amount of 138 mg. The actual experimental yield was 48.5% under optimum
conditions, which compared well with the maximum predicted value of 47.6%. |
| first_indexed | 2024-03-06T07:09:35Z |
| format | Article |
| id | upm.eprints-6325 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:09:35Z |
| publishDate | 2008 |
| publisher | Wiley Blackwell |
| record_format | dspace |
| spelling | upm.eprints-63252015-11-11T02:04:17Z http://psasir.upm.edu.my/id/eprint/6325/ Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester Yasin, Yamin Basri, Mahiran Ahmad, Faujan Salleh, Abu Bakar BACKGROUND: The synthesis of betulinic acid ester using betulinic acid and oleyl alcohol catalyzed by Novozym 435 (immobilized Candida antarctica lipase) was carried out. Response surface methodology (RSM) based on a five-level, three-variable, central composite rotatable design (CCRD) was employed to evaluate the interactive effects of various parameters. The parameters were reaction time (8–16 h), temperature (20–60 ◦C) and enzyme amount (120–160 mg). RESULTS: Simultaneously increasing reaction time, temperature and amount of enzyme increased the yields of betulinic acid ester produced. CONCLUSION: The optimum conditions derived via RSM for the reaction were reaction time of 10.2 h, temperature of 53.1 ◦C and enzyme amount of 138 mg. The actual experimental yield was 48.5% under optimum conditions, which compared well with the maximum predicted value of 47.6%. Wiley Blackwell 2008 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/6325/1/Response%20surface%20methodology%20as%20a%20tool%20to%20study%20the%20lipase.pdf Yasin, Yamin and Basri, Mahiran and Ahmad, Faujan and Salleh, Abu Bakar (2008) Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester. Journal of Chemical Technology and Biotechnology, 83 (5). pp. 694-698. ISSN 0268–2575 http://dx.doi.org/10.1002/jctb.1858 10.1002/jctb.1858 English |
| spellingShingle | Yasin, Yamin Basri, Mahiran Ahmad, Faujan Salleh, Abu Bakar Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester |
| title | Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester |
| title_full | Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester |
| title_fullStr | Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester |
| title_full_unstemmed | Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester |
| title_short | Response surface methodology as a tool to study the lipase-catalyzed synthesis of betulinic acid ester |
| title_sort | response surface methodology as a tool to study the lipase catalyzed synthesis of betulinic acid ester |
| url | http://psasir.upm.edu.my/id/eprint/6325/1/Response%20surface%20methodology%20as%20a%20tool%20to%20study%20the%20lipase.pdf |
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