Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug delivery system
Chitosan (CS) iron oxide magnetic nanoparticles (MNPs) were coated with phytic acid (PTA) to form phytic acid-chitosan-iron oxide nanocomposite (PTA-CS-MNP). The obtained nanocomposite and nanocarrier were characterized by powder X-ray diffraction, Fourier transform infrared spectroscopy, vibrating...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63345/1/Sustained%20release%20of%20anticancer%20agent%20phytic%20acid%20from%20its%20chitosan-coated%20magnetic%20nanoparticles%20for%20drug-delivery%20system.pdf |
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| author | Barahuie, Farahnaz Dorniani, Dena Saifullah, Bullo Gothai, Sivapragasam Hussein, Mohd Zobir Pandurangan, Ashok Kumar Arulselvan, Palanisamy Norhaizan, Mohd Esa |
| author_facet | Barahuie, Farahnaz Dorniani, Dena Saifullah, Bullo Gothai, Sivapragasam Hussein, Mohd Zobir Pandurangan, Ashok Kumar Arulselvan, Palanisamy Norhaizan, Mohd Esa |
| author_sort | Barahuie, Farahnaz |
| collection | UPM |
| description | Chitosan (CS) iron oxide magnetic nanoparticles (MNPs) were coated with phytic acid (PTA) to form phytic acid-chitosan-iron oxide nanocomposite (PTA-CS-MNP). The obtained nanocomposite and nanocarrier were characterized by powder X-ray diffraction, Fourier transform infrared spectroscopy, vibrating sample magnetometry, transmission electron microscopy, and thermogravimetric and differential thermogravimetric analyses. Fourier transform infrared spectra and thermal analysis of MNPs and PTA-CS-MNP nanocomposite confirmed the binding of CS on the surface of MNPs and the loading of PTA in the PTA-CS-MNP nanocomposite. The coating process enhanced the thermal stability of the anticancer nanocomposite obtained. X-ray diffraction results showed that the MNPs and PTA-CS-MNP nanocomposite are pure magnetite. Drug loading was estimated using ultraviolet-visible spectroscopy and showing a 12.9% in the designed nanocomposite. Magnetization curves demonstrated that the synthesized MNPs and nanocomposite were superparamagnetic with saturation magnetizations of 53.25 emu/g and 42.15 emu/g, respectively. The release study showed that around 86% and 93% of PTA from PTA-CS-MNP nanocomposite could be released within 127 and 56 hours by a phosphate buffer solution at pH 7.4 and 4.8, respectively, in a sustained manner and governed by pseudo-second order kinetic model. The cytotoxicity of the compounds on HT-29 colon cancer cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The HT-29 cell line was more sensitive against PTA-CS-MNP nanocomposite than PTA alone. No cytotoxic effect was observed on normal cells (3T3 fibroblast cells). This result indicates that PTA-CS-MNP nanocomposite can inhibit the proliferation of colon cancer cells without causing any harm to normal cell. |
| first_indexed | 2024-03-06T09:44:18Z |
| format | Article |
| id | upm.eprints-63345 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:44:18Z |
| publishDate | 2017 |
| publisher | Dove Medical Press |
| record_format | dspace |
| spelling | upm.eprints-633452018-10-16T02:18:22Z http://psasir.upm.edu.my/id/eprint/63345/ Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug delivery system Barahuie, Farahnaz Dorniani, Dena Saifullah, Bullo Gothai, Sivapragasam Hussein, Mohd Zobir Pandurangan, Ashok Kumar Arulselvan, Palanisamy Norhaizan, Mohd Esa Chitosan (CS) iron oxide magnetic nanoparticles (MNPs) were coated with phytic acid (PTA) to form phytic acid-chitosan-iron oxide nanocomposite (PTA-CS-MNP). The obtained nanocomposite and nanocarrier were characterized by powder X-ray diffraction, Fourier transform infrared spectroscopy, vibrating sample magnetometry, transmission electron microscopy, and thermogravimetric and differential thermogravimetric analyses. Fourier transform infrared spectra and thermal analysis of MNPs and PTA-CS-MNP nanocomposite confirmed the binding of CS on the surface of MNPs and the loading of PTA in the PTA-CS-MNP nanocomposite. The coating process enhanced the thermal stability of the anticancer nanocomposite obtained. X-ray diffraction results showed that the MNPs and PTA-CS-MNP nanocomposite are pure magnetite. Drug loading was estimated using ultraviolet-visible spectroscopy and showing a 12.9% in the designed nanocomposite. Magnetization curves demonstrated that the synthesized MNPs and nanocomposite were superparamagnetic with saturation magnetizations of 53.25 emu/g and 42.15 emu/g, respectively. The release study showed that around 86% and 93% of PTA from PTA-CS-MNP nanocomposite could be released within 127 and 56 hours by a phosphate buffer solution at pH 7.4 and 4.8, respectively, in a sustained manner and governed by pseudo-second order kinetic model. The cytotoxicity of the compounds on HT-29 colon cancer cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The HT-29 cell line was more sensitive against PTA-CS-MNP nanocomposite than PTA alone. No cytotoxic effect was observed on normal cells (3T3 fibroblast cells). This result indicates that PTA-CS-MNP nanocomposite can inhibit the proliferation of colon cancer cells without causing any harm to normal cell. Dove Medical Press 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63345/1/Sustained%20release%20of%20anticancer%20agent%20phytic%20acid%20from%20its%20chitosan-coated%20magnetic%20nanoparticles%20for%20drug-delivery%20system.pdf Barahuie, Farahnaz and Dorniani, Dena and Saifullah, Bullo and Gothai, Sivapragasam and Hussein, Mohd Zobir and Pandurangan, Ashok Kumar and Arulselvan, Palanisamy and Norhaizan, Mohd Esa (2017) Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug delivery system. International Journal of Nanomedicine, 2017 (12). 2361 - 2372. ISSN 1176-9114; ESSN: 1178-2013 10.2147/IJN.S126245 |
| spellingShingle | Barahuie, Farahnaz Dorniani, Dena Saifullah, Bullo Gothai, Sivapragasam Hussein, Mohd Zobir Pandurangan, Ashok Kumar Arulselvan, Palanisamy Norhaizan, Mohd Esa Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug delivery system |
| title | Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug delivery system |
| title_full | Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug delivery system |
| title_fullStr | Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug delivery system |
| title_full_unstemmed | Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug delivery system |
| title_short | Sustained release of anticancer agent phytic acid from its chitosan-coated magnetic nanoparticles for drug delivery system |
| title_sort | sustained release of anticancer agent phytic acid from its chitosan coated magnetic nanoparticles for drug delivery system |
| url | http://psasir.upm.edu.my/id/eprint/63345/1/Sustained%20release%20of%20anticancer%20agent%20phytic%20acid%20from%20its%20chitosan-coated%20magnetic%20nanoparticles%20for%20drug-delivery%20system.pdf |
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