The role of solvent-accessible leu-208 of cold-active Pseudomonas fluorescens strain AMS8 lipase in interfacial activation, substrate accessibility and low-molecular weight esterification in the presence of toluene
The alkaline cold-active lipase from Pseudomonas fluorescens AMS8 undergoes major structural changes when reacted with hydrophobic organic solvents. In toluene, the AMS8 lipase catalytic region is exposed by the moving hydrophobic lid 2 (Glu-148 to Gly-167). Solvent-accessible surface area analysis...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63526/1/The%20Role%20of%20Solvent-Accessible%20Leu-208%20of%20Cold-Active%20Pseudomonas%20fluorescens%20Strain%20AMS8%20Lipase%20in%20Interfacial%20Activation.pdf |