Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus
Specific-pathogen-free (SPF) chickens infected with very virulent (vv) infectious bursal disease virus (IBDV) UPM94/273 developed lower pathogenicity compared to UPM97/61. Sequence analysis indicated that UPM94/273 is an exceptional vvIBDV. In this study, a SYBRw Green I based real-time reverse tra...
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| Format: | Article |
| Language: | English English |
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Elsevier
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/6362/1/Comparative%20analysis%20of%20viral%20RNA%20and%20apoptotic%20cells%20in%20bursae%20following%20infection%20with%20infectious%20bursal%20disease%20virus.pdf |
| _version_ | 1796967149424607232 |
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| author | Lih, Ling Kong Omar, Abdul Rahman Bejo, Mohd Hair |
| author_facet | Lih, Ling Kong Omar, Abdul Rahman Bejo, Mohd Hair |
| author_sort | Lih, Ling Kong |
| collection | UPM |
| description | Specific-pathogen-free (SPF) chickens infected with very virulent (vv) infectious bursal disease virus (IBDV) UPM94/273 developed lower pathogenicity compared to UPM97/61. Sequence analysis indicated that UPM94/273 is an exceptional vvIBDV. In this study, a SYBRw Green I based
real-time reverse transcriptase reaction assay was developed to measure viral RNA in the bursae of
SPF chickens infected with IBDV. Specificity of the amplified products was confirmed by melting temperature analysis. A linear relationship was observed between the amount of input viral RNA and the threshold values for IBDV-specific product over five log10 dilutions. The viral RNA level following infection with UPM94/273 was significantly higher at day 1 and 2 post-inoculation (p.i.)
compared to UPM97/61 infected chickens. However, chickens infected with UPM97/61 had significantly higher numbers of bursal cells undergoing apoptosis compared to UPM94/273 infected chickens. In both groups, the number of apoptotic cells and viral RNA levels peak at day 3 p.i. This study indicates that UPM97/61 and UPM94/273 have different efficiency of replication and percentage of apoptotic cells in bursae during the acute phase of IBDV infection. |
| first_indexed | 2024-03-06T07:09:41Z |
| format | Article |
| id | upm.eprints-6362 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:09:41Z |
| publishDate | 2008 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-63622015-10-05T07:48:00Z http://psasir.upm.edu.my/id/eprint/6362/ Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus Lih, Ling Kong Omar, Abdul Rahman Bejo, Mohd Hair Specific-pathogen-free (SPF) chickens infected with very virulent (vv) infectious bursal disease virus (IBDV) UPM94/273 developed lower pathogenicity compared to UPM97/61. Sequence analysis indicated that UPM94/273 is an exceptional vvIBDV. In this study, a SYBRw Green I based real-time reverse transcriptase reaction assay was developed to measure viral RNA in the bursae of SPF chickens infected with IBDV. Specificity of the amplified products was confirmed by melting temperature analysis. A linear relationship was observed between the amount of input viral RNA and the threshold values for IBDV-specific product over five log10 dilutions. The viral RNA level following infection with UPM94/273 was significantly higher at day 1 and 2 post-inoculation (p.i.) compared to UPM97/61 infected chickens. However, chickens infected with UPM97/61 had significantly higher numbers of bursal cells undergoing apoptosis compared to UPM94/273 infected chickens. In both groups, the number of apoptotic cells and viral RNA levels peak at day 3 p.i. This study indicates that UPM97/61 and UPM94/273 have different efficiency of replication and percentage of apoptotic cells in bursae during the acute phase of IBDV infection. Elsevier 2008-09-01 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/6362/1/Comparative%20analysis%20of%20viral%20RNA%20and%20apoptotic%20cells%20in%20bursae%20following%20infection%20with%20infectious%20bursal%20disease%20virus.pdf Lih, Ling Kong and Omar, Abdul Rahman and Bejo, Mohd Hair (2008) Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus. Comparative Immunology, Microbiology and Infectious Diseases, 27 (5). pp. 556-563. ISSN 0147-9571 http://dx.doi.org/10.1016/j.cimid.2004.01.006 10.1016/j.cimid.2004.01.006 English |
| spellingShingle | Lih, Ling Kong Omar, Abdul Rahman Bejo, Mohd Hair Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title | Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title_full | Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title_fullStr | Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title_full_unstemmed | Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title_short | Comparative analysis of viral RNA and apoptotic cells in bursae following infection with infectious bursal disease virus |
| title_sort | comparative analysis of viral rna and apoptotic cells in bursae following infection with infectious bursal disease virus |
| url | http://psasir.upm.edu.my/id/eprint/6362/1/Comparative%20analysis%20of%20viral%20RNA%20and%20apoptotic%20cells%20in%20bursae%20following%20infection%20with%20infectious%20bursal%20disease%20virus.pdf |
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