Utilization of acid pre-treated coconut dregs as a substrate for production of detergent compatible lipase by Bacillus stratosphericus

In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in...

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Main Authors: Zin, Nur Bainun Mohd, Yusof, Busyra Mohamad, Oslan, Siti Nurbaya, Wasoh, Helmi, Joo, Shun Tan, Ariff, Arbakariya B., Halim, Murni
Format: Article
Language:English
Published: Springer Open 2017
Online Access:http://psasir.upm.edu.my/id/eprint/63664/1/Utilization%20of%20acid%20pre-treated%20coconut%20dregs%20as%20a%20substrate%20for%20production%20of%20detergent%20compatible%20lipase%20by%20Bacillus%20stratosphericus.pdf
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author Zin, Nur Bainun Mohd
Yusof, Busyra Mohamad
Oslan, Siti Nurbaya
Wasoh, Helmi
Joo, Shun Tan
Ariff, Arbakariya B.
Halim, Murni
author_facet Zin, Nur Bainun Mohd
Yusof, Busyra Mohamad
Oslan, Siti Nurbaya
Wasoh, Helmi
Joo, Shun Tan
Ariff, Arbakariya B.
Halim, Murni
author_sort Zin, Nur Bainun Mohd
collection UPM
description In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) and crude fat (9%). A newly isolated Bacillus stratosphericus has been demonstrated to perform cellulose hydrolysis on coconut dregs producing fermentable sugars. The highest extracellular lipase activity of 140 U/mL has been achieved in submerged fermentation with acid pre-treated coconut dregs. The lipase was found to be active over a wide range of temperatures and pHs. The activity of lipase can be generally increased by the presence of detergent ingredients such as Tween-80, cetyltrimethylammonium bromide, hydrogen peroxide and phosphate per sulphate. The great compatibility of lipase in commercial detergents has also underlined its potential as an additive ingredient in biodetergent formulations.
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spelling upm.eprints-636642018-11-30T03:24:39Z http://psasir.upm.edu.my/id/eprint/63664/ Utilization of acid pre-treated coconut dregs as a substrate for production of detergent compatible lipase by Bacillus stratosphericus Zin, Nur Bainun Mohd Yusof, Busyra Mohamad Oslan, Siti Nurbaya Wasoh, Helmi Joo, Shun Tan Ariff, Arbakariya B. Halim, Murni In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) and crude fat (9%). A newly isolated Bacillus stratosphericus has been demonstrated to perform cellulose hydrolysis on coconut dregs producing fermentable sugars. The highest extracellular lipase activity of 140 U/mL has been achieved in submerged fermentation with acid pre-treated coconut dregs. The lipase was found to be active over a wide range of temperatures and pHs. The activity of lipase can be generally increased by the presence of detergent ingredients such as Tween-80, cetyltrimethylammonium bromide, hydrogen peroxide and phosphate per sulphate. The great compatibility of lipase in commercial detergents has also underlined its potential as an additive ingredient in biodetergent formulations. Springer Open 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63664/1/Utilization%20of%20acid%20pre-treated%20coconut%20dregs%20as%20a%20substrate%20for%20production%20of%20detergent%20compatible%20lipase%20by%20Bacillus%20stratosphericus.pdf Zin, Nur Bainun Mohd and Yusof, Busyra Mohamad and Oslan, Siti Nurbaya and Wasoh, Helmi and Joo, Shun Tan and Ariff, Arbakariya B. and Halim, Murni (2017) Utilization of acid pre-treated coconut dregs as a substrate for production of detergent compatible lipase by Bacillus stratosphericus. AMB Express, 7 (131). pp. 1-13. ISSN 2191-0855 10.1186/s13568-017-0433-y
spellingShingle Zin, Nur Bainun Mohd
Yusof, Busyra Mohamad
Oslan, Siti Nurbaya
Wasoh, Helmi
Joo, Shun Tan
Ariff, Arbakariya B.
Halim, Murni
Utilization of acid pre-treated coconut dregs as a substrate for production of detergent compatible lipase by Bacillus stratosphericus
title Utilization of acid pre-treated coconut dregs as a substrate for production of detergent compatible lipase by Bacillus stratosphericus
title_full Utilization of acid pre-treated coconut dregs as a substrate for production of detergent compatible lipase by Bacillus stratosphericus
title_fullStr Utilization of acid pre-treated coconut dregs as a substrate for production of detergent compatible lipase by Bacillus stratosphericus
title_full_unstemmed Utilization of acid pre-treated coconut dregs as a substrate for production of detergent compatible lipase by Bacillus stratosphericus
title_short Utilization of acid pre-treated coconut dregs as a substrate for production of detergent compatible lipase by Bacillus stratosphericus
title_sort utilization of acid pre treated coconut dregs as a substrate for production of detergent compatible lipase by bacillus stratosphericus
url http://psasir.upm.edu.my/id/eprint/63664/1/Utilization%20of%20acid%20pre-treated%20coconut%20dregs%20as%20a%20substrate%20for%20production%20of%20detergent%20compatible%20lipase%20by%20Bacillus%20stratosphericus.pdf
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