Virulence genes expression among methicillin-resistant staphylococcus aureusisolated form cancer and non-cancer patients
Relative quantitative real-time reverse transcriptase polymerase chain reaction (qPCR) assay was designed and applied in order to study the expression levels of selected genes encoding the adherence and toxins virulent factors. Relative quantification qPCR showed a significant higher expression leve...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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International Journal of Recent Scientific Research
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63687/1/Virulence%20genes%20expression%20among%20methicillin-resistant%20staphylococcus%20aureusisolated%20form%20cancer%20and%20non-cancer%20patients.pdf |
| _version_ | 1796977785732857856 |
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| author | M. A, Alreshidi Neela, V. A. S., Alsharari R. A., Hamat A. A., Alsalamah O., Alzoghaibi M. N., Shamsudin |
| author_facet | M. A, Alreshidi Neela, V. A. S., Alsharari R. A., Hamat A. A., Alsalamah O., Alzoghaibi M. N., Shamsudin |
| author_sort | M. A, Alreshidi |
| collection | UPM |
| description | Relative quantitative real-time reverse transcriptase polymerase chain reaction (qPCR) assay was designed and applied in order to study the expression levels of selected genes encoding the adherence and toxins virulent factors. Relative quantification qPCR showed a significant higher expression level of common genes tested among strains isolated from cancer patients not only within the clone but also among different lineages. This study demonstrated that although all MRSA strains studied from cancer and non-cancer patients possessed several virulence determinantsthe expression rather than presence of virulence determinants may mediate higher pathogenicity potential. These data will aid in developing more effective infection control strategy to improve the management of MRSA infection in cancer patients. |
| first_indexed | 2024-03-06T09:45:10Z |
| format | Article |
| id | upm.eprints-63687 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:45:10Z |
| publishDate | 2017 |
| publisher | International Journal of Recent Scientific Research |
| record_format | dspace |
| spelling | upm.eprints-636872018-11-07T09:31:54Z http://psasir.upm.edu.my/id/eprint/63687/ Virulence genes expression among methicillin-resistant staphylococcus aureusisolated form cancer and non-cancer patients M. A, Alreshidi Neela, V. A. S., Alsharari R. A., Hamat A. A., Alsalamah O., Alzoghaibi M. N., Shamsudin Relative quantitative real-time reverse transcriptase polymerase chain reaction (qPCR) assay was designed and applied in order to study the expression levels of selected genes encoding the adherence and toxins virulent factors. Relative quantification qPCR showed a significant higher expression level of common genes tested among strains isolated from cancer patients not only within the clone but also among different lineages. This study demonstrated that although all MRSA strains studied from cancer and non-cancer patients possessed several virulence determinantsthe expression rather than presence of virulence determinants may mediate higher pathogenicity potential. These data will aid in developing more effective infection control strategy to improve the management of MRSA infection in cancer patients. International Journal of Recent Scientific Research 2017-09 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63687/1/Virulence%20genes%20expression%20among%20methicillin-resistant%20staphylococcus%20aureusisolated%20form%20cancer%20and%20non-cancer%20patients.pdf M. A, Alreshidi and Neela, V. and A. S., Alsharari and R. A., Hamat and A. A., Alsalamah and O., Alzoghaibi and M. N., Shamsudin (2017) Virulence genes expression among methicillin-resistant staphylococcus aureusisolated form cancer and non-cancer patients. International Journal of Recent Scientific Research, 8 (9). 20321 - 20327. ISSN 0976-3031 http://www.recentscientific.com/sites/default/files/8656-A-2017.pdf 10.24327/ijrsr.2017.0809.0872 |
| spellingShingle | M. A, Alreshidi Neela, V. A. S., Alsharari R. A., Hamat A. A., Alsalamah O., Alzoghaibi M. N., Shamsudin Virulence genes expression among methicillin-resistant staphylococcus aureusisolated form cancer and non-cancer patients |
| title | Virulence genes expression among methicillin-resistant staphylococcus aureusisolated form cancer and non-cancer patients |
| title_full | Virulence genes expression among methicillin-resistant staphylococcus aureusisolated form cancer and non-cancer patients |
| title_fullStr | Virulence genes expression among methicillin-resistant staphylococcus aureusisolated form cancer and non-cancer patients |
| title_full_unstemmed | Virulence genes expression among methicillin-resistant staphylococcus aureusisolated form cancer and non-cancer patients |
| title_short | Virulence genes expression among methicillin-resistant staphylococcus aureusisolated form cancer and non-cancer patients |
| title_sort | virulence genes expression among methicillin resistant staphylococcus aureusisolated form cancer and non cancer patients |
| url | http://psasir.upm.edu.my/id/eprint/63687/1/Virulence%20genes%20expression%20among%20methicillin-resistant%20staphylococcus%20aureusisolated%20form%20cancer%20and%20non-cancer%20patients.pdf |
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