Vitamin E-mediated modulation of glutamate receptor expression in an oxidative stress model of neural cells derived from embryonic stem cell cultures
Glutamate is the primary excitatory neurotransmitter in the central nervous system. Excessive concentrations of glutamate in the brain can be excitotoxic and cause oxidative stress, which is associated with Alzheimer’s disease. In the present study, the effects of vitamin E in the form of tocotrieno...
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| Format: | Article |
| Language: | English |
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Hindawi Publishing Corporation
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63692/1/Vitamin%20E-Mediated%20Modulation%20of%20Glutamate%20Receptor%20Expression%20in%20an%20Oxidative%20Stress%20Model%20of%20Neural%20Cells.pdf |
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| author | Jalil, Afifah Abd Khaza’ai, Huzwah Nordin, Norshariza Mansor, Nur’izzati Zaulkffali, Amirah Salwani |
| author_facet | Jalil, Afifah Abd Khaza’ai, Huzwah Nordin, Norshariza Mansor, Nur’izzati Zaulkffali, Amirah Salwani |
| author_sort | Jalil, Afifah Abd |
| collection | UPM |
| description | Glutamate is the primary excitatory neurotransmitter in the central nervous system. Excessive concentrations of glutamate in the brain can be excitotoxic and cause oxidative stress, which is associated with Alzheimer’s disease. In the present study, the effects of vitamin E in the form of tocotrienol-rich fraction (TRF) and alpha-tocopherol (α-TCP) in modulating the glutamate receptor and neuron injury markers in an in vitro model of oxidative stress in neural-derived embryonic stem (ES) cell cultures were elucidated. A transgenic mouse ES cell line (46C) was differentiated into a neural lineage in vitro via induction with retinoic acid. These cells were then subjected to oxidative stress with a significantly high concentration of glutamate. Measurement of reactive oxygen species (ROS) was performed after inducing glutamate excitotoxicity, and recovery from this toxicity in response to vitamin E was determined. The gene expression levels of glutamate receptors and neuron-specific enolase were elucidated using real-time PCR. The results reveal that neural cells derived from 46C cells and subjected to oxidative stress exhibit downregulation of NMDA, kainate receptor, and NSE after posttreatment with different concentrations of TRF and α-TCP, a sign of neurorecovery. Treatment of either TRF or α-TCP reduced the levels of ROS in neural cells subjected to glutamate-induced oxidative stress; these results indicated that vitamin E is a potent antioxidant. |
| first_indexed | 2024-03-06T09:45:10Z |
| format | Article |
| id | upm.eprints-63692 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:45:10Z |
| publishDate | 2017 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | upm.eprints-636922018-11-07T09:23:46Z http://psasir.upm.edu.my/id/eprint/63692/ Vitamin E-mediated modulation of glutamate receptor expression in an oxidative stress model of neural cells derived from embryonic stem cell cultures Jalil, Afifah Abd Khaza’ai, Huzwah Nordin, Norshariza Mansor, Nur’izzati Zaulkffali, Amirah Salwani Glutamate is the primary excitatory neurotransmitter in the central nervous system. Excessive concentrations of glutamate in the brain can be excitotoxic and cause oxidative stress, which is associated with Alzheimer’s disease. In the present study, the effects of vitamin E in the form of tocotrienol-rich fraction (TRF) and alpha-tocopherol (α-TCP) in modulating the glutamate receptor and neuron injury markers in an in vitro model of oxidative stress in neural-derived embryonic stem (ES) cell cultures were elucidated. A transgenic mouse ES cell line (46C) was differentiated into a neural lineage in vitro via induction with retinoic acid. These cells were then subjected to oxidative stress with a significantly high concentration of glutamate. Measurement of reactive oxygen species (ROS) was performed after inducing glutamate excitotoxicity, and recovery from this toxicity in response to vitamin E was determined. The gene expression levels of glutamate receptors and neuron-specific enolase were elucidated using real-time PCR. The results reveal that neural cells derived from 46C cells and subjected to oxidative stress exhibit downregulation of NMDA, kainate receptor, and NSE after posttreatment with different concentrations of TRF and α-TCP, a sign of neurorecovery. Treatment of either TRF or α-TCP reduced the levels of ROS in neural cells subjected to glutamate-induced oxidative stress; these results indicated that vitamin E is a potent antioxidant. Hindawi Publishing Corporation 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63692/1/Vitamin%20E-Mediated%20Modulation%20of%20Glutamate%20Receptor%20Expression%20in%20an%20Oxidative%20Stress%20Model%20of%20Neural%20Cells.pdf Jalil, Afifah Abd and Khaza’ai, Huzwah and Nordin, Norshariza and Mansor, Nur’izzati and Zaulkffali, Amirah Salwani (2017) Vitamin E-mediated modulation of glutamate receptor expression in an oxidative stress model of neural cells derived from embryonic stem cell cultures. Evidence-Based Complementary and Alternative Medicine. art. no. 6048936. pp. 1-12. ISSN 1741-427X; ESSN: 1741-4288 https://www.hindawi.com/journals/ecam/2017/6048936/cta/ 10.1155/2017/6048936 |
| spellingShingle | Jalil, Afifah Abd Khaza’ai, Huzwah Nordin, Norshariza Mansor, Nur’izzati Zaulkffali, Amirah Salwani Vitamin E-mediated modulation of glutamate receptor expression in an oxidative stress model of neural cells derived from embryonic stem cell cultures |
| title | Vitamin E-mediated modulation of glutamate receptor expression in an oxidative stress model of neural cells derived from embryonic stem cell cultures |
| title_full | Vitamin E-mediated modulation of glutamate receptor expression in an oxidative stress model of neural cells derived from embryonic stem cell cultures |
| title_fullStr | Vitamin E-mediated modulation of glutamate receptor expression in an oxidative stress model of neural cells derived from embryonic stem cell cultures |
| title_full_unstemmed | Vitamin E-mediated modulation of glutamate receptor expression in an oxidative stress model of neural cells derived from embryonic stem cell cultures |
| title_short | Vitamin E-mediated modulation of glutamate receptor expression in an oxidative stress model of neural cells derived from embryonic stem cell cultures |
| title_sort | vitamin e mediated modulation of glutamate receptor expression in an oxidative stress model of neural cells derived from embryonic stem cell cultures |
| url | http://psasir.upm.edu.my/id/eprint/63692/1/Vitamin%20E-Mediated%20Modulation%20of%20Glutamate%20Receptor%20Expression%20in%20an%20Oxidative%20Stress%20Model%20of%20Neural%20Cells.pdf |
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