Zn2+ cross-linked sodium alginate-g-allylamine-mannose polymeric carrier of rifampicin for macrophage targeting tuberculosis nanotherapy
Our aim was to evaluate the capacity of polymeric nanoparticles (PNPs) to selectively deliver an antituberculosis drug (rifampicin; RF) to alveolar macrophages. Anionic biodegradable copolymer sodium alginate-g-allylamine-mannose (SA-g-AA-M) was synthesized by atom transfer free radical polymerizati...
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| Format: | Article |
| Language: | English |
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Royal Society of Chemistry
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63743/1/Zn2%2B%20cross-linked%20sodium%20alginate-g-allylamine-mannose%20polymeric%20carrier%20of%20rifampicin%20for%20macrophage%20targeting%20tuberculosis%20nanotherapy.pdf |
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| author | Praphakar, Rajendran Amarnath Munusamy, Murugan A. Alarfaj, Abdullah A. Kumar, Suresh Rajan, Mariappan |
| author_facet | Praphakar, Rajendran Amarnath Munusamy, Murugan A. Alarfaj, Abdullah A. Kumar, Suresh Rajan, Mariappan |
| author_sort | Praphakar, Rajendran Amarnath |
| collection | UPM |
| description | Our aim was to evaluate the capacity of polymeric nanoparticles (PNPs) to selectively deliver an antituberculosis drug (rifampicin; RF) to alveolar macrophages. Anionic biodegradable copolymer sodium alginate-g-allylamine-mannose (SA-g-AA-M) was synthesized by atom transfer free radical polymerization and direct coupling of the respective conjugates. The fabrication of RF-loaded Zn2+ ion-cross-linked SA-g-AA-M PNPs was conducted by an O/W emulsion method followed by ionotropic gelation. The structural nature of the RF-loaded SA-g-AA-M PNPs was analyzed by Fourier transform infrared (FT-IR) spectroscopy. Meanwhile, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to illustrate the shape and morphology of the nanoparticles. The PNPs were observed as uniform spheres in the nanometer range (<300 nm), with a low polydispersity index, and excellent performance in terms of drug encapsulation and release ability. The PNPs also showed strong antimicrobial activities against Mycobacterium tuberculosis. Cytotoxicity evaluation in VERO cells by an MTT assay suggested that the PNPs have good biocompatibility. Alveolar macrophage targeting was evaluated via cellular uptake by A549 cells. The cellular uptake results revealed that the Zn2+ concentration of the PNPs increases the intracellular concentration of RF and enhances its antitubercular efficiency. Overall, the results suggest that PNPs could lead to the development of a possible mannose-containing carrier for a macrophage-targeting drug delivery system. |
| first_indexed | 2024-03-06T09:45:17Z |
| format | Article |
| id | upm.eprints-63743 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:45:17Z |
| publishDate | 2017 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | upm.eprints-637432018-11-30T04:14:51Z http://psasir.upm.edu.my/id/eprint/63743/ Zn2+ cross-linked sodium alginate-g-allylamine-mannose polymeric carrier of rifampicin for macrophage targeting tuberculosis nanotherapy Praphakar, Rajendran Amarnath Munusamy, Murugan A. Alarfaj, Abdullah A. Kumar, Suresh Rajan, Mariappan Our aim was to evaluate the capacity of polymeric nanoparticles (PNPs) to selectively deliver an antituberculosis drug (rifampicin; RF) to alveolar macrophages. Anionic biodegradable copolymer sodium alginate-g-allylamine-mannose (SA-g-AA-M) was synthesized by atom transfer free radical polymerization and direct coupling of the respective conjugates. The fabrication of RF-loaded Zn2+ ion-cross-linked SA-g-AA-M PNPs was conducted by an O/W emulsion method followed by ionotropic gelation. The structural nature of the RF-loaded SA-g-AA-M PNPs was analyzed by Fourier transform infrared (FT-IR) spectroscopy. Meanwhile, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to illustrate the shape and morphology of the nanoparticles. The PNPs were observed as uniform spheres in the nanometer range (<300 nm), with a low polydispersity index, and excellent performance in terms of drug encapsulation and release ability. The PNPs also showed strong antimicrobial activities against Mycobacterium tuberculosis. Cytotoxicity evaluation in VERO cells by an MTT assay suggested that the PNPs have good biocompatibility. Alveolar macrophage targeting was evaluated via cellular uptake by A549 cells. The cellular uptake results revealed that the Zn2+ concentration of the PNPs increases the intracellular concentration of RF and enhances its antitubercular efficiency. Overall, the results suggest that PNPs could lead to the development of a possible mannose-containing carrier for a macrophage-targeting drug delivery system. Royal Society of Chemistry 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63743/1/Zn2%2B%20cross-linked%20sodium%20alginate-g-allylamine-mannose%20polymeric%20carrier%20of%20rifampicin%20for%20macrophage%20targeting%20tuberculosis%20nanotherapy.pdf Praphakar, Rajendran Amarnath and Munusamy, Murugan A. and Alarfaj, Abdullah A. and Kumar, Suresh and Rajan, Mariappan (2017) Zn2+ cross-linked sodium alginate-g-allylamine-mannose polymeric carrier of rifampicin for macrophage targeting tuberculosis nanotherapy. New Journal of Chemistry, 41 (19). pp. 1-13. ISSN 1144-0546; ESSN: 1369-9261 10.1039/C7NJ01808H |
| spellingShingle | Praphakar, Rajendran Amarnath Munusamy, Murugan A. Alarfaj, Abdullah A. Kumar, Suresh Rajan, Mariappan Zn2+ cross-linked sodium alginate-g-allylamine-mannose polymeric carrier of rifampicin for macrophage targeting tuberculosis nanotherapy |
| title | Zn2+ cross-linked sodium alginate-g-allylamine-mannose polymeric carrier of rifampicin for macrophage targeting tuberculosis nanotherapy |
| title_full | Zn2+ cross-linked sodium alginate-g-allylamine-mannose polymeric carrier of rifampicin for macrophage targeting tuberculosis nanotherapy |
| title_fullStr | Zn2+ cross-linked sodium alginate-g-allylamine-mannose polymeric carrier of rifampicin for macrophage targeting tuberculosis nanotherapy |
| title_full_unstemmed | Zn2+ cross-linked sodium alginate-g-allylamine-mannose polymeric carrier of rifampicin for macrophage targeting tuberculosis nanotherapy |
| title_short | Zn2+ cross-linked sodium alginate-g-allylamine-mannose polymeric carrier of rifampicin for macrophage targeting tuberculosis nanotherapy |
| title_sort | zn2 cross linked sodium alginate g allylamine mannose polymeric carrier of rifampicin for macrophage targeting tuberculosis nanotherapy |
| url | http://psasir.upm.edu.my/id/eprint/63743/1/Zn2%2B%20cross-linked%20sodium%20alginate-g-allylamine-mannose%20polymeric%20carrier%20of%20rifampicin%20for%20macrophage%20targeting%20tuberculosis%20nanotherapy.pdf |
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