Protein microarray-based IgE immunoassay for allergy diagnosis
Protein microarray is a miniaturized multi-analyte, solid-phased immunoassay where thousands of immobilized individual protein spots on a microscopic slide bind are bound to specific antibodies (immunoglobulins) from serum samples, which are then detected by fluorescent labeling. The image processin...
| Main Authors: | , , |
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| Format: | Book Section |
| Language: | English |
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Humana Press
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63822/1/Protein%20Microarray-Based%20IgE%20Immunoassay%20for%20Allergy%20Diagnosis.pdf |
| _version_ | 1796977798025314304 |
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| author | Jambari, Nuzul Noorahya Wang, Xioa Wei Alcocer, Marcos |
| author_facet | Jambari, Nuzul Noorahya Wang, Xioa Wei Alcocer, Marcos |
| author_sort | Jambari, Nuzul Noorahya |
| collection | UPM |
| description | Protein microarray is a miniaturized multi-analyte, solid-phased immunoassay where thousands of immobilized individual protein spots on a microscopic slide bind are bound to specific antibodies (immunoglobulins) from serum samples, which are then detected by fluorescent labeling. The image processing and pattern recognition are then quantitatively analyzed using advanced algorithms. Here, we describe the use of an in-house-produced complex protein microarray containing extracts and pure proteins that has been probed with antibodies present in the horse sera and detection by fluorophore-conjugated antibody and data analysis. The flexibility of the number and types of proteins that can be printed on the microarray allows different set of specific IgE immunoassay analysis to be carried out. |
| first_indexed | 2024-03-06T09:45:21Z |
| format | Book Section |
| id | upm.eprints-63822 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:45:21Z |
| publishDate | 2017 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | upm.eprints-638222018-07-20T08:30:04Z http://psasir.upm.edu.my/id/eprint/63822/ Protein microarray-based IgE immunoassay for allergy diagnosis Jambari, Nuzul Noorahya Wang, Xioa Wei Alcocer, Marcos Protein microarray is a miniaturized multi-analyte, solid-phased immunoassay where thousands of immobilized individual protein spots on a microscopic slide bind are bound to specific antibodies (immunoglobulins) from serum samples, which are then detected by fluorescent labeling. The image processing and pattern recognition are then quantitatively analyzed using advanced algorithms. Here, we describe the use of an in-house-produced complex protein microarray containing extracts and pure proteins that has been probed with antibodies present in the horse sera and detection by fluorophore-conjugated antibody and data analysis. The flexibility of the number and types of proteins that can be printed on the microarray allows different set of specific IgE immunoassay analysis to be carried out. Humana Press 2017-03 Book Section PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63822/1/Protein%20Microarray-Based%20IgE%20Immunoassay%20for%20Allergy%20Diagnosis.pdf Jambari, Nuzul Noorahya and Wang, Xioa Wei and Alcocer, Marcos (2017) Protein microarray-based IgE immunoassay for allergy diagnosis. In: Food Allergens: Methods and Protocols. Methods in Molecular Biology . Humana Press, New York, 129 - 137. ISBN 9781493969258 10.1007/978-1-4939-6925-8_10 |
| spellingShingle | Jambari, Nuzul Noorahya Wang, Xioa Wei Alcocer, Marcos Protein microarray-based IgE immunoassay for allergy diagnosis |
| title | Protein microarray-based IgE immunoassay for allergy diagnosis |
| title_full | Protein microarray-based IgE immunoassay for allergy diagnosis |
| title_fullStr | Protein microarray-based IgE immunoassay for allergy diagnosis |
| title_full_unstemmed | Protein microarray-based IgE immunoassay for allergy diagnosis |
| title_short | Protein microarray-based IgE immunoassay for allergy diagnosis |
| title_sort | protein microarray based ige immunoassay for allergy diagnosis |
| url | http://psasir.upm.edu.my/id/eprint/63822/1/Protein%20Microarray-Based%20IgE%20Immunoassay%20for%20Allergy%20Diagnosis.pdf |
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