Evaluation of factors affecting polyhydroxyalkanoates production by Comamonas sp. EB172 using central composite design
Aims: Statistical approach, central composite design (CCD) was used to investigate the complex interaction among temperature (25-37 °C), initial medium pH (5-9), inoculum size (4-10 % (v/v)), concentration of (NH 4) 2SO 4 (0-1 g/L) and concentration of mixed organic acids (5-10 g/L) in the productio...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Malaysian Society for Microbiology
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/63948/1/Evaluation%20of%20factors%20affecting%20polyhydroxyalkanoates%20production%20by%20Comamonas%20sp.%20EB172%20using%20central%20composite%20design.pdf |
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| author | Mohd Johar, Noor Azman Hassan, Mohd Ali Zakaria @ Mamat, Mohd Rafein Phang, Lai Yee Shirai, Yoshihito Ariffin, Hidayah |
| author_facet | Mohd Johar, Noor Azman Hassan, Mohd Ali Zakaria @ Mamat, Mohd Rafein Phang, Lai Yee Shirai, Yoshihito Ariffin, Hidayah |
| author_sort | Mohd Johar, Noor Azman |
| collection | UPM |
| description | Aims: Statistical approach, central composite design (CCD) was used to investigate the complex interaction among temperature (25-37 °C), initial medium pH (5-9), inoculum size (4-10 % (v/v)), concentration of (NH 4) 2SO 4 (0-1 g/L) and concentration of mixed organic acids (5-10 g/L) in the production of polyhydroxyalkanoates by Comamonas sp. EB172.
Methodology and Results: Mixed organic acids derived from anaerobically treated palm oil mill effluent (POME) containing acetic:propionic:butyric (ratio of 3:1:1) were used as carbon source in the batch culture of Comamonas sp. EB172 to produce polyhydoxyalkanoates (PHAs). The analysis of variance (ANOVA) showed that all five factors were significantly important in the batch fermentation by shake flask with a P value of less than 0.001. The optimal temperature, initial medium pH, inoculum size, concentration of (NH 4) 2SO 4 and concentration of mixed organic acids were 30 °C, 7.04, 4.0 % (v/v), 0.01 g/L and 5.05 g/L respectively.
Conclusion, significance and impact of study: Optimization of the production medium containing mixed organic acids has improved the PHA production for more than 2 folds. Under optimal condition in the shake flask fermentation, the predicted growth is 2.98 g/L of dry cell weight (DCW) with 47.07 wt % of PHA content. The highest yield of PHA was 0.28 g of PHA per g mixed organic acids. |
| first_indexed | 2024-03-06T09:45:22Z |
| format | Article |
| id | upm.eprints-63948 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:45:22Z |
| publishDate | 2012 |
| publisher | Malaysian Society for Microbiology |
| record_format | dspace |
| spelling | upm.eprints-639482018-06-08T00:25:24Z http://psasir.upm.edu.my/id/eprint/63948/ Evaluation of factors affecting polyhydroxyalkanoates production by Comamonas sp. EB172 using central composite design Mohd Johar, Noor Azman Hassan, Mohd Ali Zakaria @ Mamat, Mohd Rafein Phang, Lai Yee Shirai, Yoshihito Ariffin, Hidayah Aims: Statistical approach, central composite design (CCD) was used to investigate the complex interaction among temperature (25-37 °C), initial medium pH (5-9), inoculum size (4-10 % (v/v)), concentration of (NH 4) 2SO 4 (0-1 g/L) and concentration of mixed organic acids (5-10 g/L) in the production of polyhydroxyalkanoates by Comamonas sp. EB172. Methodology and Results: Mixed organic acids derived from anaerobically treated palm oil mill effluent (POME) containing acetic:propionic:butyric (ratio of 3:1:1) were used as carbon source in the batch culture of Comamonas sp. EB172 to produce polyhydoxyalkanoates (PHAs). The analysis of variance (ANOVA) showed that all five factors were significantly important in the batch fermentation by shake flask with a P value of less than 0.001. The optimal temperature, initial medium pH, inoculum size, concentration of (NH 4) 2SO 4 and concentration of mixed organic acids were 30 °C, 7.04, 4.0 % (v/v), 0.01 g/L and 5.05 g/L respectively. Conclusion, significance and impact of study: Optimization of the production medium containing mixed organic acids has improved the PHA production for more than 2 folds. Under optimal condition in the shake flask fermentation, the predicted growth is 2.98 g/L of dry cell weight (DCW) with 47.07 wt % of PHA content. The highest yield of PHA was 0.28 g of PHA per g mixed organic acids. Malaysian Society for Microbiology 2012 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/63948/1/Evaluation%20of%20factors%20affecting%20polyhydroxyalkanoates%20production%20by%20Comamonas%20sp.%20EB172%20using%20central%20composite%20design.pdf Mohd Johar, Noor Azman and Hassan, Mohd Ali and Zakaria @ Mamat, Mohd Rafein and Phang, Lai Yee and Shirai, Yoshihito and Ariffin, Hidayah (2012) Evaluation of factors affecting polyhydroxyalkanoates production by Comamonas sp. EB172 using central composite design. Malaysian Journal of Microbiology, 8 (3). pp. 184-190. ISSN 1823-8262; ESSN: 2231-7538 http://mjm.usm.my/index.php?r=cms/entry/view&id=51 10.21161/mjm.04612 |
| spellingShingle | Mohd Johar, Noor Azman Hassan, Mohd Ali Zakaria @ Mamat, Mohd Rafein Phang, Lai Yee Shirai, Yoshihito Ariffin, Hidayah Evaluation of factors affecting polyhydroxyalkanoates production by Comamonas sp. EB172 using central composite design |
| title | Evaluation of factors affecting polyhydroxyalkanoates production by Comamonas sp. EB172 using central composite design |
| title_full | Evaluation of factors affecting polyhydroxyalkanoates production by Comamonas sp. EB172 using central composite design |
| title_fullStr | Evaluation of factors affecting polyhydroxyalkanoates production by Comamonas sp. EB172 using central composite design |
| title_full_unstemmed | Evaluation of factors affecting polyhydroxyalkanoates production by Comamonas sp. EB172 using central composite design |
| title_short | Evaluation of factors affecting polyhydroxyalkanoates production by Comamonas sp. EB172 using central composite design |
| title_sort | evaluation of factors affecting polyhydroxyalkanoates production by comamonas sp eb172 using central composite design |
| url | http://psasir.upm.edu.my/id/eprint/63948/1/Evaluation%20of%20factors%20affecting%20polyhydroxyalkanoates%20production%20by%20Comamonas%20sp.%20EB172%20using%20central%20composite%20design.pdf |
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