Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12
In this study, a novel glyphosate-degrading shows the ability to reduce molybdenum to molybdenum blue. The enzyme from this bacterium was partially purified and partially characterized to ascertain whether the Mo-reducing enzyme from this bacterium shows better or lower efficiency in reducing molybd...
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| 格式: | 文件 |
| 语言: | English |
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Hibiscus Publisher
2016
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| 在线阅读: | http://psasir.upm.edu.my/id/eprint/64006/1/Partial%20purification%20and%20characterization%20of%20the%20molybdenum-reducing%20enzyme%20from%20the%20glyphosate-degrading%20Burkholderia%20vietnamiensis%20strain%20AQ5-12.pdf |
| _version_ | 1825932668135538688 |
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| author | Jamulidin, Siti Nur Khadijah Manogaran, Motharasan Yakasai, Mohd Hafeez Abdul Rahman, Mohd Fadhil Abd. Shukor, Mohd. Yunus |
| author_facet | Jamulidin, Siti Nur Khadijah Manogaran, Motharasan Yakasai, Mohd Hafeez Abdul Rahman, Mohd Fadhil Abd. Shukor, Mohd. Yunus |
| author_sort | Jamulidin, Siti Nur Khadijah |
| collection | UPM |
| description | In this study, a novel glyphosate-degrading shows the ability to reduce molybdenum to molybdenum blue. The enzyme from this bacterium was partially purified and partially characterized to ascertain whether the Mo-reducing enzyme from this bacterium shows better or lower efficiency in reducing molybdenum compared to other Mo-reducing bacterium that only exhibits a single biotransformation activity. The enzyme was partially purified using ammonium sulphate fractionation. The Vmax for the electron donating substrate or NADH was at 1.905 nmole Mo blue/min while the Km was 6.146 mM. The regression coefficient was 0.98. Comparative assessment with the previously characterized Mo-reducing enzyme from various bacteria showed that the Mo-reducing enzyme from Burkholderia vietnamiensis strain AQ5-12 showed a lower enzyme activity. |
| first_indexed | 2024-03-06T09:45:33Z |
| format | Article |
| id | upm.eprints-64006 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:45:33Z |
| publishDate | 2016 |
| publisher | Hibiscus Publisher |
| record_format | dspace |
| spelling | upm.eprints-640062018-06-08T00:45:01Z http://psasir.upm.edu.my/id/eprint/64006/ Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12 Jamulidin, Siti Nur Khadijah Manogaran, Motharasan Yakasai, Mohd Hafeez Abdul Rahman, Mohd Fadhil Abd. Shukor, Mohd. Yunus In this study, a novel glyphosate-degrading shows the ability to reduce molybdenum to molybdenum blue. The enzyme from this bacterium was partially purified and partially characterized to ascertain whether the Mo-reducing enzyme from this bacterium shows better or lower efficiency in reducing molybdenum compared to other Mo-reducing bacterium that only exhibits a single biotransformation activity. The enzyme was partially purified using ammonium sulphate fractionation. The Vmax for the electron donating substrate or NADH was at 1.905 nmole Mo blue/min while the Km was 6.146 mM. The regression coefficient was 0.98. Comparative assessment with the previously characterized Mo-reducing enzyme from various bacteria showed that the Mo-reducing enzyme from Burkholderia vietnamiensis strain AQ5-12 showed a lower enzyme activity. Hibiscus Publisher 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64006/1/Partial%20purification%20and%20characterization%20of%20the%20molybdenum-reducing%20enzyme%20from%20the%20glyphosate-degrading%20Burkholderia%20vietnamiensis%20strain%20AQ5-12.pdf Jamulidin, Siti Nur Khadijah and Manogaran, Motharasan and Yakasai, Mohd Hafeez and Abdul Rahman, Mohd Fadhil and Abd. Shukor, Mohd. Yunus (2016) Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12. Bioremediation Science and Technology Research, 4 (1). pp. 7-9. ISSN 2289-5892 https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/363 |
| spellingShingle | Jamulidin, Siti Nur Khadijah Manogaran, Motharasan Yakasai, Mohd Hafeez Abdul Rahman, Mohd Fadhil Abd. Shukor, Mohd. Yunus Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12 |
| title | Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12 |
| title_full | Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12 |
| title_fullStr | Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12 |
| title_full_unstemmed | Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12 |
| title_short | Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12 |
| title_sort | partial purification and characterization of the molybdenum reducing enzyme from the glyphosate degrading burkholderia vietnamiensis strain aq5 12 |
| url | http://psasir.upm.edu.my/id/eprint/64006/1/Partial%20purification%20and%20characterization%20of%20the%20molybdenum-reducing%20enzyme%20from%20the%20glyphosate-degrading%20Burkholderia%20vietnamiensis%20strain%20AQ5-12.pdf |
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