Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP

Petroleum hydrocarbons remain as the major contaminants that could be found across the world. Remediation approach through the utilisation of microbes as the bioremediation means is widely recognised due to their outstanding values. As a result, scientific reports on the isolation and identification...

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Main Authors: Abdul Habib, Nur Muhamad Syahir, Wan Johari, Wan Lutfi, Abd. Shukor, Mohd. Yunus, Yasid, Nur Adeela
Format: Article
Language:English
Published: Hibiscus Publisher 2017
Online Access:http://psasir.upm.edu.my/id/eprint/64009/1/Screening%20of%20hydrocarbon-degrading%20bacterial%20isolates%20using%20the%20redox%20application%20of%202%2C6-DCPIP.pdf
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author Abdul Habib, Nur Muhamad Syahir
Wan Johari, Wan Lutfi
Abd. Shukor, Mohd. Yunus
Yasid, Nur Adeela
author_facet Abdul Habib, Nur Muhamad Syahir
Wan Johari, Wan Lutfi
Abd. Shukor, Mohd. Yunus
Yasid, Nur Adeela
author_sort Abdul Habib, Nur Muhamad Syahir
collection UPM
description Petroleum hydrocarbons remain as the major contaminants that could be found across the world. Remediation approach through the utilisation of microbes as the bioremediation means is widely recognised due to their outstanding values. As a result, scientific reports on the isolation and identification of new hydrocarbon-degrading strains were on the rise. Colourimetric-based assays are one of the fastest methods to identify the capability of hydrocarbon-degrading strains in both qualitative and quantitative assessment. In this study, the hydrocarbon-degrading potential of nine bacterial isolates was observed via 2,6-dichlorophenolindophenol (DCPIP) test. Two potent diesel-utilising isolates show a distinctive tendency to utilise aromatic (ADL15) and aliphatic (ADL36) hydrocarbons. Both isolates prove to be a good candidate for bioremediation of wide range of petroleum hydrocarbon components.
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spelling upm.eprints-640092018-06-08T00:44:55Z http://psasir.upm.edu.my/id/eprint/64009/ Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP Abdul Habib, Nur Muhamad Syahir Wan Johari, Wan Lutfi Abd. Shukor, Mohd. Yunus Yasid, Nur Adeela Petroleum hydrocarbons remain as the major contaminants that could be found across the world. Remediation approach through the utilisation of microbes as the bioremediation means is widely recognised due to their outstanding values. As a result, scientific reports on the isolation and identification of new hydrocarbon-degrading strains were on the rise. Colourimetric-based assays are one of the fastest methods to identify the capability of hydrocarbon-degrading strains in both qualitative and quantitative assessment. In this study, the hydrocarbon-degrading potential of nine bacterial isolates was observed via 2,6-dichlorophenolindophenol (DCPIP) test. Two potent diesel-utilising isolates show a distinctive tendency to utilise aromatic (ADL15) and aliphatic (ADL36) hydrocarbons. Both isolates prove to be a good candidate for bioremediation of wide range of petroleum hydrocarbon components. Hibiscus Publisher 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64009/1/Screening%20of%20hydrocarbon-degrading%20bacterial%20isolates%20using%20the%20redox%20application%20of%202%2C6-DCPIP.pdf Abdul Habib, Nur Muhamad Syahir and Wan Johari, Wan Lutfi and Abd. Shukor, Mohd. Yunus and Yasid, Nur Adeela (2017) Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP. Bioremediation Science and Technology Research, 5 (2). pp. 13-16. ISSN 2289-5892 https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/358
spellingShingle Abdul Habib, Nur Muhamad Syahir
Wan Johari, Wan Lutfi
Abd. Shukor, Mohd. Yunus
Yasid, Nur Adeela
Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP
title Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP
title_full Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP
title_fullStr Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP
title_full_unstemmed Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP
title_short Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP
title_sort screening of hydrocarbon degrading bacterial isolates using the redox application of 2 6 dcpip
url http://psasir.upm.edu.my/id/eprint/64009/1/Screening%20of%20hydrocarbon-degrading%20bacterial%20isolates%20using%20the%20redox%20application%20of%202%2C6-DCPIP.pdf
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