Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus
Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesser known property of AChE is its inhibition by heavy metals. In this work we evaluate an AChE from brains of striped snakehead (Channa striatus) wastes from aquaculture industry as an inhibitive assay for heav...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64015/1/Assay%20for%20heavy%20metals%20using%20an%20inhibitive%20assay%20based%20on%20the%20acetylcholinesterase%20from%20Channa%20striatus.pdf |
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| author | Zulkifli, Ain Farihah Tham, Lik Gin Perumal, Natarajan Sabullah, Mohd Khalizan Mohamad Azzeme, Azzreena Abd. Shukor, Mohd. Yunus Shaharuddin, Noor Azmi |
| author_facet | Zulkifli, Ain Farihah Tham, Lik Gin Perumal, Natarajan Sabullah, Mohd Khalizan Mohamad Azzeme, Azzreena Abd. Shukor, Mohd. Yunus Shaharuddin, Noor Azmi |
| author_sort | Zulkifli, Ain Farihah |
| collection | UPM |
| description | Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesser known property of AChE is its inhibition by heavy metals. In this work we evaluate an AChE from brains of striped snakehead (Channa striatus) wastes from aquaculture industry as an inhibitive assay for heavy metals. We discovered that the AChE was inhibited almost completely by Hg2+, Ag2+ and Cu2+ during an initial screening. When tested at various concentrations, the heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 for the heavy metals Hg2+, Ag2+, Pb2+, Cu2+ and Cr6+ were 0.08432, 0.1008, 0.1255, 0.0871, and 0.1771, respectively. The IC50 for these heavy metals are comparable and some are lower than the IC50 values from the cholinesterases from previously studied fish. The assay can be carried out in less than 30 minutes at ambient temperature. |
| first_indexed | 2024-03-06T09:45:34Z |
| format | Article |
| id | upm.eprints-64015 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:45:34Z |
| publishDate | 2017 |
| publisher | Hibiscus Publisher |
| record_format | dspace |
| spelling | upm.eprints-640152018-06-08T00:52:08Z http://psasir.upm.edu.my/id/eprint/64015/ Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus Zulkifli, Ain Farihah Tham, Lik Gin Perumal, Natarajan Sabullah, Mohd Khalizan Mohamad Azzeme, Azzreena Abd. Shukor, Mohd. Yunus Shaharuddin, Noor Azmi Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesser known property of AChE is its inhibition by heavy metals. In this work we evaluate an AChE from brains of striped snakehead (Channa striatus) wastes from aquaculture industry as an inhibitive assay for heavy metals. We discovered that the AChE was inhibited almost completely by Hg2+, Ag2+ and Cu2+ during an initial screening. When tested at various concentrations, the heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 for the heavy metals Hg2+, Ag2+, Pb2+, Cu2+ and Cr6+ were 0.08432, 0.1008, 0.1255, 0.0871, and 0.1771, respectively. The IC50 for these heavy metals are comparable and some are lower than the IC50 values from the cholinesterases from previously studied fish. The assay can be carried out in less than 30 minutes at ambient temperature. Hibiscus Publisher 2017 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64015/1/Assay%20for%20heavy%20metals%20using%20an%20inhibitive%20assay%20based%20on%20the%20acetylcholinesterase%20from%20Channa%20striatus.pdf Zulkifli, Ain Farihah and Tham, Lik Gin and Perumal, Natarajan and Sabullah, Mohd Khalizan and Mohamad Azzeme, Azzreena and Abd. Shukor, Mohd. Yunus and Shaharuddin, Noor Azmi (2017) Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus. Bioremediation Science and Technology Research, 5 (1). pp. 7-11. ISSN 2289-5892 https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/351 |
| spellingShingle | Zulkifli, Ain Farihah Tham, Lik Gin Perumal, Natarajan Sabullah, Mohd Khalizan Mohamad Azzeme, Azzreena Abd. Shukor, Mohd. Yunus Shaharuddin, Noor Azmi Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus |
| title | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus |
| title_full | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus |
| title_fullStr | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus |
| title_full_unstemmed | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus |
| title_short | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus |
| title_sort | assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from channa striatus |
| url | http://psasir.upm.edu.my/id/eprint/64015/1/Assay%20for%20heavy%20metals%20using%20an%20inhibitive%20assay%20based%20on%20the%20acetylcholinesterase%20from%20Channa%20striatus.pdf |
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