Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus
Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from brains of Clarias batrachus (catfish) exposed to wastes from aquaculture industry as an inhibitive assay for...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64020/1/Assay%20for%20heavy%20metals%20using%20an%20inhibitive%20assay%20based%20on%20the%20acetylcholinesterase%20from%20Clarias%20batrachus.pdf |
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| author | Muhammad, Umar Abubakar Tham, Lik Gin Perumal, Natarajan Mohd Daud, Hassan Yasid, Nur Adeela Abd. Shukor, Mohd. Yunus |
| author_facet | Muhammad, Umar Abubakar Tham, Lik Gin Perumal, Natarajan Mohd Daud, Hassan Yasid, Nur Adeela Abd. Shukor, Mohd. Yunus |
| author_sort | Muhammad, Umar Abubakar |
| collection | UPM |
| description | Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from brains of Clarias batrachus (catfish) exposed to wastes from aquaculture industry as an inhibitive assay for heavy metals. We discovered that the AChE was inhibited completely by Hg2+, Ag2+, Pb2+, Cu2+, Cd2+, Cr6+ and Zn2+ during initial screening. When tested at various concentrations, the heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 (mg/L) for the heavy metals Ag2+, Cu2+, Hg2+, Cr6+ and Cd2+ were 0.088, 0.078, 0.071, 0.87 and 0.913, respectively. The IC50 for these heavy metals are comparable, and some are lower than the IC50 values from the cholinesterases from previously studied fish. The assay can be carried out in less than 30 minutes at ambient temperature. |
| first_indexed | 2024-03-06T09:45:35Z |
| format | Article |
| id | upm.eprints-64020 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:45:35Z |
| publishDate | 2016 |
| publisher | Hibiscus Publisher |
| record_format | dspace |
| spelling | upm.eprints-640202018-06-08T00:51:57Z http://psasir.upm.edu.my/id/eprint/64020/ Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus Muhammad, Umar Abubakar Tham, Lik Gin Perumal, Natarajan Mohd Daud, Hassan Yasid, Nur Adeela Abd. Shukor, Mohd. Yunus Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesser-known property of AChE is its inhibition by heavy metals. In this work, we evaluate an AChE from brains of Clarias batrachus (catfish) exposed to wastes from aquaculture industry as an inhibitive assay for heavy metals. We discovered that the AChE was inhibited completely by Hg2+, Ag2+, Pb2+, Cu2+, Cd2+, Cr6+ and Zn2+ during initial screening. When tested at various concentrations, the heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 (mg/L) for the heavy metals Ag2+, Cu2+, Hg2+, Cr6+ and Cd2+ were 0.088, 0.078, 0.071, 0.87 and 0.913, respectively. The IC50 for these heavy metals are comparable, and some are lower than the IC50 values from the cholinesterases from previously studied fish. The assay can be carried out in less than 30 minutes at ambient temperature. Hibiscus Publisher 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64020/1/Assay%20for%20heavy%20metals%20using%20an%20inhibitive%20assay%20based%20on%20the%20acetylcholinesterase%20from%20Clarias%20batrachus.pdf Muhammad, Umar Abubakar and Tham, Lik Gin and Perumal, Natarajan and Mohd Daud, Hassan and Yasid, Nur Adeela and Abd. Shukor, Mohd. Yunus (2016) Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus. Bioremediation Science and Technology Research, 4 (2). pp. 6-10. ISSN 2289-5892 https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/369 |
| spellingShingle | Muhammad, Umar Abubakar Tham, Lik Gin Perumal, Natarajan Mohd Daud, Hassan Yasid, Nur Adeela Abd. Shukor, Mohd. Yunus Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus |
| title | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus |
| title_full | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus |
| title_fullStr | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus |
| title_full_unstemmed | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus |
| title_short | Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Clarias batrachus |
| title_sort | assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from clarias batrachus |
| url | http://psasir.upm.edu.my/id/eprint/64020/1/Assay%20for%20heavy%20metals%20using%20an%20inhibitive%20assay%20based%20on%20the%20acetylcholinesterase%20from%20Clarias%20batrachus.pdf |
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