Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna
Molybdenum is an emerging pollutant. Bioremediation of this heavy metal is possible by the mediation of Mo-reducing bacteria. These bacteria contain the Mo-reducing enzymes that can conver toxic soluble molybdenum into molybdenum blue; a less soluble and less toxic form of the metal. To date only th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hibiscus Publisher
2016
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64027/1/Partial%20purification%20of%20the%20molybdenum-reducing%20enzyme%20from%20Bacillus%20pumilus%20strain%20Lbna.pdf |
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| author | Abo-Shakeer, Lubna Kamil Abdulhussein Yakasai, Mohd Hafeez Abdul Rahman, Mohd Fadhil Abu Bakar, Nurlizah Syed, Mohd Arif Abdullah, Norhani Othman, Ahmad Razi |
| author_facet | Abo-Shakeer, Lubna Kamil Abdulhussein Yakasai, Mohd Hafeez Abdul Rahman, Mohd Fadhil Abu Bakar, Nurlizah Syed, Mohd Arif Abdullah, Norhani Othman, Ahmad Razi |
| author_sort | Abo-Shakeer, Lubna Kamil Abdulhussein |
| collection | UPM |
| description | Molybdenum is an emerging pollutant. Bioremediation of this heavy metal is possible by the mediation of Mo-reducing bacteria. These bacteria contain the Mo-reducing enzymes that can conver toxic soluble molybdenum into molybdenum blue; a less soluble and less toxic form of the metal. To date only the enzyme has been purified from only one bacterium. The aim of this study is to purify the Mo-reducing enzyme from a previously isolated Mo-reducing bacterium Bacillus pumilus strain Lbna using ammonium sulphate fractionation followed by ion exchange and then gel filtration. Two clear bands were obtained after the gel filtration step with molecular weights of 70 and 100 kDa. This indicates that further additional purification methods need to be used to get a purified fraction. Hence, additional steps of chromatography such as hydroxyapatite or chromatofocusing techniques can be applied in the future. |
| first_indexed | 2024-03-06T09:45:36Z |
| format | Article |
| id | upm.eprints-64027 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:45:36Z |
| publishDate | 2016 |
| publisher | Hibiscus Publisher |
| record_format | dspace |
| spelling | upm.eprints-640272018-06-08T00:51:44Z http://psasir.upm.edu.my/id/eprint/64027/ Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna Abo-Shakeer, Lubna Kamil Abdulhussein Yakasai, Mohd Hafeez Abdul Rahman, Mohd Fadhil Abu Bakar, Nurlizah Syed, Mohd Arif Abdullah, Norhani Othman, Ahmad Razi Molybdenum is an emerging pollutant. Bioremediation of this heavy metal is possible by the mediation of Mo-reducing bacteria. These bacteria contain the Mo-reducing enzymes that can conver toxic soluble molybdenum into molybdenum blue; a less soluble and less toxic form of the metal. To date only the enzyme has been purified from only one bacterium. The aim of this study is to purify the Mo-reducing enzyme from a previously isolated Mo-reducing bacterium Bacillus pumilus strain Lbna using ammonium sulphate fractionation followed by ion exchange and then gel filtration. Two clear bands were obtained after the gel filtration step with molecular weights of 70 and 100 kDa. This indicates that further additional purification methods need to be used to get a purified fraction. Hence, additional steps of chromatography such as hydroxyapatite or chromatofocusing techniques can be applied in the future. Hibiscus Publisher 2016 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64027/1/Partial%20purification%20of%20the%20molybdenum-reducing%20enzyme%20from%20Bacillus%20pumilus%20strain%20Lbna.pdf Abo-Shakeer, Lubna Kamil Abdulhussein and Yakasai, Mohd Hafeez and Abdul Rahman, Mohd Fadhil and Abu Bakar, Nurlizah and Syed, Mohd Arif and Abdullah, Norhani and Othman, Ahmad Razi (2016) Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna. Bioremediation Science and Technology Research, 4 (1). pp. 14-17. ISSN 2289-5892 https://journal.hibiscuspublisher.com/index.php/BSTR/article/view/366 |
| spellingShingle | Abo-Shakeer, Lubna Kamil Abdulhussein Yakasai, Mohd Hafeez Abdul Rahman, Mohd Fadhil Abu Bakar, Nurlizah Syed, Mohd Arif Abdullah, Norhani Othman, Ahmad Razi Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna |
| title | Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna |
| title_full | Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna |
| title_fullStr | Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna |
| title_full_unstemmed | Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna |
| title_short | Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna |
| title_sort | partial purification of the molybdenum reducing enzyme from bacillus pumilus strain lbna |
| url | http://psasir.upm.edu.my/id/eprint/64027/1/Partial%20purification%20of%20the%20molybdenum-reducing%20enzyme%20from%20Bacillus%20pumilus%20strain%20Lbna.pdf |
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