Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea
An efficient and reproducible method for large-scale propagation of Phalaenopsis gigantea using leaf sections has been developed. Leaf sections from young plants were cultured on New Dogashima medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN),...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Sarawak Biodiversity Centre
2008
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64277/1/PROCEEDING_100719-14.pdf |
| _version_ | 1825932719670951936 |
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| author | Niknejad, Azadeh Abdul Kadir, Mihdzar Kadzimin, Saleh Abdullah, N. A. |
| author_facet | Niknejad, Azadeh Abdul Kadir, Mihdzar Kadzimin, Saleh Abdullah, N. A. |
| author_sort | Niknejad, Azadeh |
| collection | UPM |
| description | An efficient and reproducible method for large-scale propagation of Phalaenopsis gigantea using leaf sections has been developed. Leaf sections from young plants were cultured on New Dogashima medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mgL-1) alone and in combinations with (auxins anaphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mgL-1). The explants developed calli and protocorm-like-bodies (PLBs) within 8 weeks of culture. Treatment TDZ in combination with auxins was found to be best for the induction of callus and PLBs. |
| first_indexed | 2024-03-06T09:46:19Z |
| format | Conference or Workshop Item |
| id | upm.eprints-64277 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:46:19Z |
| publishDate | 2008 |
| publisher | Sarawak Biodiversity Centre |
| record_format | dspace |
| spelling | upm.eprints-642772018-07-04T02:31:37Z http://psasir.upm.edu.my/id/eprint/64277/ Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea Niknejad, Azadeh Abdul Kadir, Mihdzar Kadzimin, Saleh Abdullah, N. A. An efficient and reproducible method for large-scale propagation of Phalaenopsis gigantea using leaf sections has been developed. Leaf sections from young plants were cultured on New Dogashima medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mgL-1) alone and in combinations with (auxins anaphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mgL-1). The explants developed calli and protocorm-like-bodies (PLBs) within 8 weeks of culture. Treatment TDZ in combination with auxins was found to be best for the induction of callus and PLBs. Sarawak Biodiversity Centre 2008 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64277/1/PROCEEDING_100719-14.pdf Niknejad, Azadeh and Abdul Kadir, Mihdzar and Kadzimin, Saleh and Abdullah, N. A. (2008) Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea. In: Biodiversity and Biotechnology Symposium 2008, 19-21 Nov. 2008, Kuching, Sarawak, Malaysia. (pp. 428-431). |
| spellingShingle | Niknejad, Azadeh Abdul Kadir, Mihdzar Kadzimin, Saleh Abdullah, N. A. Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea |
| title | Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea |
| title_full | Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea |
| title_fullStr | Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea |
| title_full_unstemmed | Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea |
| title_short | Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea |
| title_sort | induction of callus and protocorm like bodies plbs in phalaenopsis gigantea |
| url | http://psasir.upm.edu.my/id/eprint/64277/1/PROCEEDING_100719-14.pdf |
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