Laccase enzyme production by Pycnoporus sanguineus using oil palm empty fruit bunch as substrate
Laccase is one of the ligninolytic enzymes along with manganese peroxidase and lignin peroxidase. The biotechnological use of laccase has been widely applied in various field ranging from discoloration of textile dye effluent, pulp and paper processing, removal of phenolics from wine and many more....
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/64350/1/BIO%20Poster%20111117%2010.pdf |
| _version_ | 1796977880466456576 |
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| author | Masran, Ruqayyah Kamal Bahrin, Ezyana Ibrahim, Mohamad Faizal Phang, Lai Yee Abd. Aziz, Suraini |
| author_facet | Masran, Ruqayyah Kamal Bahrin, Ezyana Ibrahim, Mohamad Faizal Phang, Lai Yee Abd. Aziz, Suraini |
| author_sort | Masran, Ruqayyah |
| collection | UPM |
| description | Laccase is one of the ligninolytic enzymes along with manganese peroxidase and lignin peroxidase. The biotechnological use of laccase has been widely applied in various field ranging from discoloration of textile dye effluent, pulp and paper processing, removal of phenolics from wine and many more. The white rot basidiomycete fungus Pycnoporus sanguineus is reported to be one of the most prominent laccase producers. Over the last few decades, many efforts have been done to improve the laccase enzyme production. The addition of inducer is expected to increase the laccase production as it will induce high expression of laccase gene by the fungus. Different types of inducer which are veratryl alcohol, copper sulfate, 2,5-xylidine and ferulic acid were investigated in this study using oil palm empty fruit bunch (OPEFB) as substrate in a submerged fermentation condition. Each inducer was added at certain concentration into the enzyme production medium using one factor at a time approach. An increase in laccase enzyme activity was observed with the addition of inducer into the basal medium. Hence, laccase production by Pycnoporus sanguineus can be improved with the addition of inducer. |
| first_indexed | 2024-03-06T09:46:32Z |
| format | Conference or Workshop Item |
| id | upm.eprints-64350 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:46:32Z |
| publishDate | 2017 |
| record_format | dspace |
| spelling | upm.eprints-643502018-07-04T02:39:01Z http://psasir.upm.edu.my/id/eprint/64350/ Laccase enzyme production by Pycnoporus sanguineus using oil palm empty fruit bunch as substrate Masran, Ruqayyah Kamal Bahrin, Ezyana Ibrahim, Mohamad Faizal Phang, Lai Yee Abd. Aziz, Suraini Laccase is one of the ligninolytic enzymes along with manganese peroxidase and lignin peroxidase. The biotechnological use of laccase has been widely applied in various field ranging from discoloration of textile dye effluent, pulp and paper processing, removal of phenolics from wine and many more. The white rot basidiomycete fungus Pycnoporus sanguineus is reported to be one of the most prominent laccase producers. Over the last few decades, many efforts have been done to improve the laccase enzyme production. The addition of inducer is expected to increase the laccase production as it will induce high expression of laccase gene by the fungus. Different types of inducer which are veratryl alcohol, copper sulfate, 2,5-xylidine and ferulic acid were investigated in this study using oil palm empty fruit bunch (OPEFB) as substrate in a submerged fermentation condition. Each inducer was added at certain concentration into the enzyme production medium using one factor at a time approach. An increase in laccase enzyme activity was observed with the addition of inducer into the basal medium. Hence, laccase production by Pycnoporus sanguineus can be improved with the addition of inducer. 2017 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/64350/1/BIO%20Poster%20111117%2010.pdf Masran, Ruqayyah and Kamal Bahrin, Ezyana and Ibrahim, Mohamad Faizal and Phang, Lai Yee and Abd. Aziz, Suraini (2017) Laccase enzyme production by Pycnoporus sanguineus using oil palm empty fruit bunch as substrate. In: 5th International Symposium on Applied Engineering and Sciences (SAES2017), 14-15 Nov. 2017, Universiti Putra Malaysia. (p. 10). |
| spellingShingle | Masran, Ruqayyah Kamal Bahrin, Ezyana Ibrahim, Mohamad Faizal Phang, Lai Yee Abd. Aziz, Suraini Laccase enzyme production by Pycnoporus sanguineus using oil palm empty fruit bunch as substrate |
| title | Laccase enzyme production by Pycnoporus sanguineus using oil palm empty fruit bunch as substrate |
| title_full | Laccase enzyme production by Pycnoporus sanguineus using oil palm empty fruit bunch as substrate |
| title_fullStr | Laccase enzyme production by Pycnoporus sanguineus using oil palm empty fruit bunch as substrate |
| title_full_unstemmed | Laccase enzyme production by Pycnoporus sanguineus using oil palm empty fruit bunch as substrate |
| title_short | Laccase enzyme production by Pycnoporus sanguineus using oil palm empty fruit bunch as substrate |
| title_sort | laccase enzyme production by pycnoporus sanguineus using oil palm empty fruit bunch as substrate |
| url | http://psasir.upm.edu.my/id/eprint/64350/1/BIO%20Poster%20111117%2010.pdf |
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