Bacteriophage as carrier for multiple DNA vaccine in poultry
Bacteriophage-mediated immunization is a novel vaccine technology for DNA-vaccine delivery which has many advantages over traditional vaccines. The risk of reversion is absent, it can induce both humoral and cellular immunity, large-scale production is cheaper and its transportation is easier as the...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
Faculty of Veterinary Medicine, Universiti Putra Malaysia
2015
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| Online Access: | http://psasir.upm.edu.my/id/eprint/65019/1/PA-20.pdf |
| _version_ | 1796978014938988544 |
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| author | Al-Tayyar, O. B. Siang, T. W. Omar, Abdul Rahman Ideris, Aini |
| author_facet | Al-Tayyar, O. B. Siang, T. W. Omar, Abdul Rahman Ideris, Aini |
| author_sort | Al-Tayyar, O. B. |
| collection | UPM |
| description | Bacteriophage-mediated immunization is a novel vaccine technology for DNA-vaccine delivery which has many advantages over traditional vaccines. The risk of reversion is absent, it can induce both humoral and cellular immunity, large-scale production is cheaper and its transportation is easier as there are no particular storage requirements. After viral RNA extraction, the specific genes were amplified using reverse-transcriptase polymerase chain reaction (RT-PCR). The amplified PCR products were inserted into bacteriophage lambda vector and successful recombinant were confirmed by white-blue screening, colony-PCR and sequencing. The aim of this study is to produce a multiple bacteriophage-mediated DNA-vaccine for poultry production. |
| first_indexed | 2024-03-06T09:48:30Z |
| format | Conference or Workshop Item |
| id | upm.eprints-65019 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:48:30Z |
| publishDate | 2015 |
| publisher | Faculty of Veterinary Medicine, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-650192018-09-03T04:41:40Z http://psasir.upm.edu.my/id/eprint/65019/ Bacteriophage as carrier for multiple DNA vaccine in poultry Al-Tayyar, O. B. Siang, T. W. Omar, Abdul Rahman Ideris, Aini Bacteriophage-mediated immunization is a novel vaccine technology for DNA-vaccine delivery which has many advantages over traditional vaccines. The risk of reversion is absent, it can induce both humoral and cellular immunity, large-scale production is cheaper and its transportation is easier as there are no particular storage requirements. After viral RNA extraction, the specific genes were amplified using reverse-transcriptase polymerase chain reaction (RT-PCR). The amplified PCR products were inserted into bacteriophage lambda vector and successful recombinant were confirmed by white-blue screening, colony-PCR and sequencing. The aim of this study is to produce a multiple bacteriophage-mediated DNA-vaccine for poultry production. Faculty of Veterinary Medicine, Universiti Putra Malaysia 2015 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65019/1/PA-20.pdf Al-Tayyar, O. B. and Siang, T. W. and Omar, Abdul Rahman and Ideris, Aini (2015) Bacteriophage as carrier for multiple DNA vaccine in poultry. In: World Veterinary Poultry Association (Malaysia Branch) and World's Poultry Science Association (Malaysia Branch) Scientific Conference 2015, 21-22 Sept. 2015, Kuala Lumpur Convention Centre, Kuala Lumpur, Malaysia. (pp. 129-131). |
| spellingShingle | Al-Tayyar, O. B. Siang, T. W. Omar, Abdul Rahman Ideris, Aini Bacteriophage as carrier for multiple DNA vaccine in poultry |
| title | Bacteriophage as carrier for multiple DNA vaccine in poultry |
| title_full | Bacteriophage as carrier for multiple DNA vaccine in poultry |
| title_fullStr | Bacteriophage as carrier for multiple DNA vaccine in poultry |
| title_full_unstemmed | Bacteriophage as carrier for multiple DNA vaccine in poultry |
| title_short | Bacteriophage as carrier for multiple DNA vaccine in poultry |
| title_sort | bacteriophage as carrier for multiple dna vaccine in poultry |
| url | http://psasir.upm.edu.my/id/eprint/65019/1/PA-20.pdf |
| work_keys_str_mv | AT altayyarob bacteriophageascarrierformultiplednavaccineinpoultry AT siangtw bacteriophageascarrierformultiplednavaccineinpoultry AT omarabdulrahman bacteriophageascarrierformultiplednavaccineinpoultry AT iderisaini bacteriophageascarrierformultiplednavaccineinpoultry |