Protective effect of Centella asiatica extract and powder on oxidative stress in rats

The effect of Centella asiatica extract and powder in reducing oxidative stress in SpraqueDawley rats was evaluated. Lipid peroxidation was monitored by measuring malonaldehyde (MDA) level in blood. Activities of free radical-scavenging enzymes (superoxide dismutase and catalase) were determined u...

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Main Authors: Hussin, Mahanom, Abdul Hamid, Azizah, Mohamad, Suhaila, Saari, Nazamid, Ismail, Maznah, Bejo, Mohd Hair
Format: Article
Language:English
English
Published: Elsevier 2007
Online Access:http://psasir.upm.edu.my/id/eprint/6509/1/Protective%20effect%20of%20Centella%20asiatica%20extract%20and%20powder%20on%20oxidative%20stress%20in%20rats.pdf
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author Hussin, Mahanom
Abdul Hamid, Azizah
Mohamad, Suhaila
Saari, Nazamid
Ismail, Maznah
Bejo, Mohd Hair
author_facet Hussin, Mahanom
Abdul Hamid, Azizah
Mohamad, Suhaila
Saari, Nazamid
Ismail, Maznah
Bejo, Mohd Hair
author_sort Hussin, Mahanom
collection UPM
description The effect of Centella asiatica extract and powder in reducing oxidative stress in SpraqueDawley rats was evaluated. Lipid peroxidation was monitored by measuring malonaldehyde (MDA) level in blood. Activities of free radical-scavenging enzymes (superoxide dismutase and catalase) were determined using H2O2 decomposition and nitrobluetetrazolium reduction, respectively. Results showed that administration of H2O2 (0.1%) in drinking water of the rats, for 25 weeks, increased the malonaldehyde levels in erythrocytes of all the rats. However, rats receiving C. asiatica extract, powder and a-tocopherol had lower MDA levels than did the other rats, which indicates, decrease lipid peroxidation in these rats. Increase in catalase activity of the rats appears to be a response to H2O2 accumulation. The decrease in the activity of superoxide dismutase in C. asiatica- and a-tocopherol supplemented rats suggested a lower requirement for the enzyme and this indicates the protective effect of the plant in combating oxidative stress undergone by the rats. Results revealed that C. asiatica extract and powder may ameliorate H2O2-induced oxidative stress by decreasing lipid peroxidation via alteration of the antioxidant defence system of the rats.
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spelling upm.eprints-65092015-09-17T03:52:09Z http://psasir.upm.edu.my/id/eprint/6509/ Protective effect of Centella asiatica extract and powder on oxidative stress in rats Hussin, Mahanom Abdul Hamid, Azizah Mohamad, Suhaila Saari, Nazamid Ismail, Maznah Bejo, Mohd Hair The effect of Centella asiatica extract and powder in reducing oxidative stress in SpraqueDawley rats was evaluated. Lipid peroxidation was monitored by measuring malonaldehyde (MDA) level in blood. Activities of free radical-scavenging enzymes (superoxide dismutase and catalase) were determined using H2O2 decomposition and nitrobluetetrazolium reduction, respectively. Results showed that administration of H2O2 (0.1%) in drinking water of the rats, for 25 weeks, increased the malonaldehyde levels in erythrocytes of all the rats. However, rats receiving C. asiatica extract, powder and a-tocopherol had lower MDA levels than did the other rats, which indicates, decrease lipid peroxidation in these rats. Increase in catalase activity of the rats appears to be a response to H2O2 accumulation. The decrease in the activity of superoxide dismutase in C. asiatica- and a-tocopherol supplemented rats suggested a lower requirement for the enzyme and this indicates the protective effect of the plant in combating oxidative stress undergone by the rats. Results revealed that C. asiatica extract and powder may ameliorate H2O2-induced oxidative stress by decreasing lipid peroxidation via alteration of the antioxidant defence system of the rats. Elsevier 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/6509/1/Protective%20effect%20of%20Centella%20asiatica%20extract%20and%20powder%20on%20oxidative%20stress%20in%20rats.pdf Hussin, Mahanom and Abdul Hamid, Azizah and Mohamad, Suhaila and Saari, Nazamid and Ismail, Maznah and Bejo, Mohd Hair (2007) Protective effect of Centella asiatica extract and powder on oxidative stress in rats. Food Chemistry, 100 (2). pp. 535-541. ISSN 0308-8146 http://dx.doi.org/10.1016/j.foodchem.2005.10.022 10.1016/j.foodchem.2005.10.022 English
spellingShingle Hussin, Mahanom
Abdul Hamid, Azizah
Mohamad, Suhaila
Saari, Nazamid
Ismail, Maznah
Bejo, Mohd Hair
Protective effect of Centella asiatica extract and powder on oxidative stress in rats
title Protective effect of Centella asiatica extract and powder on oxidative stress in rats
title_full Protective effect of Centella asiatica extract and powder on oxidative stress in rats
title_fullStr Protective effect of Centella asiatica extract and powder on oxidative stress in rats
title_full_unstemmed Protective effect of Centella asiatica extract and powder on oxidative stress in rats
title_short Protective effect of Centella asiatica extract and powder on oxidative stress in rats
title_sort protective effect of centella asiatica extract and powder on oxidative stress in rats
url http://psasir.upm.edu.my/id/eprint/6509/1/Protective%20effect%20of%20Centella%20asiatica%20extract%20and%20powder%20on%20oxidative%20stress%20in%20rats.pdf
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