Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus
The sensitivity of SYBR Green 1 based real-time PCR to detect infectious bursal disease virus (IBDV) was compared with an ELISA nested RT-PCR and conventional gel electrophoresis methods. The real-time PCR was at least 10 fold more sensitive than the ELISA nested RT-PCR, while the ELISA nested PCR w...
| Principais autores: | , , , |
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| Formato: | Conference or Workshop Item |
| Idioma: | English |
| Publicado em: |
Universiti Putra Malaysia Press
2004
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| Acesso em linha: | http://psasir.upm.edu.my/id/eprint/65106/1/71-15.pdf |
| _version_ | 1825932882129977344 |
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| author | Hamzah, Hairul Aini Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini |
| author_facet | Hamzah, Hairul Aini Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini |
| author_sort | Hamzah, Hairul Aini |
| collection | UPM |
| description | The sensitivity of SYBR Green 1 based real-time PCR to detect infectious bursal disease virus (IBDV) was compared with an ELISA nested RT-PCR and conventional gel electrophoresis methods. The real-time PCR was at least 10 fold more sensitive than the ELISA nested RT-PCR, while the ELISA nested PCR was at least 10 fold more sensitive than the conventional gel electrophoresis. Thus, real-time PCR using SYBR Green 1 offers a sensitive, rapid and convenient method for screening large number of IBDV. |
| first_indexed | 2024-03-06T09:48:46Z |
| format | Conference or Workshop Item |
| id | upm.eprints-65106 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:48:46Z |
| publishDate | 2004 |
| publisher | Universiti Putra Malaysia Press |
| record_format | dspace |
| spelling | upm.eprints-651062018-09-04T04:01:51Z http://psasir.upm.edu.my/id/eprint/65106/ Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus Hamzah, Hairul Aini Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini The sensitivity of SYBR Green 1 based real-time PCR to detect infectious bursal disease virus (IBDV) was compared with an ELISA nested RT-PCR and conventional gel electrophoresis methods. The real-time PCR was at least 10 fold more sensitive than the ELISA nested RT-PCR, while the ELISA nested PCR was at least 10 fold more sensitive than the conventional gel electrophoresis. Thus, real-time PCR using SYBR Green 1 offers a sensitive, rapid and convenient method for screening large number of IBDV. Universiti Putra Malaysia Press 2004 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65106/1/71-15.pdf Hamzah, Hairul Aini and Omar, Abdul Rahman and Bejo, Mohd Hair and Ideris, Aini (2004) Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus. In: 11th International Conference of the Association of Institutions for Tropical Veterinary Medicine and 16th Veterinary Association Malaysia Congress, 23-27 Aug. 2004, Sunway Pyramid Convention Centre, Petaling Jaya, Malaysia. (pp. 226-227). |
| spellingShingle | Hamzah, Hairul Aini Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus |
| title | Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus |
| title_full | Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus |
| title_fullStr | Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus |
| title_full_unstemmed | Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus |
| title_short | Performance of real-time polymerase chain reaction in the detection of infectious bursal disease virus |
| title_sort | performance of real time polymerase chain reaction in the detection of infectious bursal disease virus |
| url | http://psasir.upm.edu.my/id/eprint/65106/1/71-15.pdf |
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