Analysis of viral RNA and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus
Previously we have shown that the very virulent (vv) infectious bursal disease virus (IBDV) UPM94/273 and UPM91/61 have significantly distinct pathogenicity and genetic profiles. In this study, a SYBR‚ Green I based real-time reverse transcriptase reaction assay was developed to measure viral RNA in...
| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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Universiti Putra Malaysia Press
2004
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| Online Access: | http://psasir.upm.edu.my/id/eprint/65107/1/71-16.pdf |
| _version_ | 1796978034549456896 |
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| author | Kong, Lih Ling Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini Seow, Heng Fong |
| author_facet | Kong, Lih Ling Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini Seow, Heng Fong |
| author_sort | Kong, Lih Ling |
| collection | UPM |
| description | Previously we have shown that the very virulent (vv) infectious bursal disease virus (IBDV) UPM94/273 and UPM91/61 have significantly distinct pathogenicity and genetic profiles. In this study, a SYBR‚ Green I based real-time reverse transcriptase reaction assay was developed to measure viral RNA in the bursae of chickens infected with IBDV. A linear relationship was observed between the amount of input viral RNA and the threshold values for IBDV-specific product over five log10 dilutions. The viral RNA level following infection with UPM94/273 was significantly higher at day 1 and 2 post-inoculation (p.i) compared to UPM97/61 infected chickens. However, chickens infected with UPM97/61 had significantly higher numbers of bursal cells undergoing apoptosis compared to UPM94/273 infected chickens. In both groups the number of apoptotic cells and viral RNA levels peak at day 3 p.i. This study indicates UPM97/61 and UPM94/273 have different efficiency of replication and percentage of apoptotic cells in bursae during the acute phase of IBDV infection. |
| first_indexed | 2024-03-06T09:48:46Z |
| format | Conference or Workshop Item |
| id | upm.eprints-65107 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:48:46Z |
| publishDate | 2004 |
| publisher | Universiti Putra Malaysia Press |
| record_format | dspace |
| spelling | upm.eprints-651072018-09-04T04:01:49Z http://psasir.upm.edu.my/id/eprint/65107/ Analysis of viral RNA and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus Kong, Lih Ling Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini Seow, Heng Fong Previously we have shown that the very virulent (vv) infectious bursal disease virus (IBDV) UPM94/273 and UPM91/61 have significantly distinct pathogenicity and genetic profiles. In this study, a SYBR‚ Green I based real-time reverse transcriptase reaction assay was developed to measure viral RNA in the bursae of chickens infected with IBDV. A linear relationship was observed between the amount of input viral RNA and the threshold values for IBDV-specific product over five log10 dilutions. The viral RNA level following infection with UPM94/273 was significantly higher at day 1 and 2 post-inoculation (p.i) compared to UPM97/61 infected chickens. However, chickens infected with UPM97/61 had significantly higher numbers of bursal cells undergoing apoptosis compared to UPM94/273 infected chickens. In both groups the number of apoptotic cells and viral RNA levels peak at day 3 p.i. This study indicates UPM97/61 and UPM94/273 have different efficiency of replication and percentage of apoptotic cells in bursae during the acute phase of IBDV infection. Universiti Putra Malaysia Press 2004 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65107/1/71-16.pdf Kong, Lih Ling and Omar, Abdul Rahman and Bejo, Mohd Hair and Ideris, Aini and Seow, Heng Fong (2004) Analysis of viral RNA and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus. In: 11th International Conference of the Association of Institutions for Tropical Veterinary Medicine and 16th Veterinary Association Malaysia Congress, 23-27 Aug. 2004, Sunway Pyramid Convention Centre, Petaling Jaya, Malaysia. (pp. 228-229). |
| spellingShingle | Kong, Lih Ling Omar, Abdul Rahman Bejo, Mohd Hair Ideris, Aini Seow, Heng Fong Analysis of viral RNA and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus |
| title | Analysis of viral RNA and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus |
| title_full | Analysis of viral RNA and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus |
| title_fullStr | Analysis of viral RNA and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus |
| title_full_unstemmed | Analysis of viral RNA and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus |
| title_short | Analysis of viral RNA and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus |
| title_sort | analysis of viral rna and apoptotic cells in bursae following infection with vaccine and very virulent strains of infectious bursal disease virus |
| url | http://psasir.upm.edu.my/id/eprint/65107/1/71-16.pdf |
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