Comparative biodiversity and effect of different media on growth and astaxanthin content of nine geographical strains of Haematococcus pluvialis
Haematococcus pluvialis is a unicellular green volvocale alga living in temporary shallow freshwater ponds. It has many applications for humans, poultry and fishes due to its ability to produce astaxanthin. The main objective of this study was to evaluate the biodiversity and growth of nine strains...
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| Format: | Article |
| Language: | English |
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Academic Journals
2012
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| Online Access: | http://psasir.upm.edu.my/id/eprint/65146/1/65146.pdf |
| _version_ | 1825932890099154944 |
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| author | Noroozi, Mostafa Omar, Hishamuddin Napis, Suhaimi Hejazi, Mohammad Amin Tan, Soon Guan |
| author_facet | Noroozi, Mostafa Omar, Hishamuddin Napis, Suhaimi Hejazi, Mohammad Amin Tan, Soon Guan |
| author_sort | Noroozi, Mostafa |
| collection | UPM |
| description | Haematococcus pluvialis is a unicellular green volvocale alga living in temporary shallow freshwater ponds. It has many applications for humans, poultry and fishes due to its ability to produce astaxanthin. The main objective of this study was to evaluate the biodiversity and growth of nine strains of H. pluvialis originating from three countries: Iran, USA and Finland in distinct growth media (Bold, NIES, OHM, Mixotroph and COMBO) and their molecular marker investigation. The biomass, astaxanthin and chlorophyll concentration were determined in all strains. The results of this study showed that Mixotroph growth medium produced the highest biomass of 0.577 g dry weight/l followed by COMBO and OHM media, respectively. However, the highest astaxanthin concentration was obtained from COMBO and OHM growth media and Iran4 and USA3 strains. Hence, the Mixotroph is suitable for biomass production and OHM or COMBO is appropriate for astaxanthin production; so two steps cultivation is feasible to produce astaxanthin. The results depicted geographical differentiations of the strains with Iran4 and USA2 strains producing the highest biomass and Finland2 strain showing the slowest one. The inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) molecular markers were used as suitable tools for the purpose of finding out genetic variations and genetic discrimination of H. pluvialis strains. This technique differentiated the strains based on their geographical status. |
| first_indexed | 2024-03-06T09:48:53Z |
| format | Article |
| id | upm.eprints-65146 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:48:53Z |
| publishDate | 2012 |
| publisher | Academic Journals |
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| spelling | upm.eprints-651462018-09-04T04:32:12Z http://psasir.upm.edu.my/id/eprint/65146/ Comparative biodiversity and effect of different media on growth and astaxanthin content of nine geographical strains of Haematococcus pluvialis Noroozi, Mostafa Omar, Hishamuddin Napis, Suhaimi Hejazi, Mohammad Amin Tan, Soon Guan Haematococcus pluvialis is a unicellular green volvocale alga living in temporary shallow freshwater ponds. It has many applications for humans, poultry and fishes due to its ability to produce astaxanthin. The main objective of this study was to evaluate the biodiversity and growth of nine strains of H. pluvialis originating from three countries: Iran, USA and Finland in distinct growth media (Bold, NIES, OHM, Mixotroph and COMBO) and their molecular marker investigation. The biomass, astaxanthin and chlorophyll concentration were determined in all strains. The results of this study showed that Mixotroph growth medium produced the highest biomass of 0.577 g dry weight/l followed by COMBO and OHM media, respectively. However, the highest astaxanthin concentration was obtained from COMBO and OHM growth media and Iran4 and USA3 strains. Hence, the Mixotroph is suitable for biomass production and OHM or COMBO is appropriate for astaxanthin production; so two steps cultivation is feasible to produce astaxanthin. The results depicted geographical differentiations of the strains with Iran4 and USA2 strains producing the highest biomass and Finland2 strain showing the slowest one. The inter-simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) molecular markers were used as suitable tools for the purpose of finding out genetic variations and genetic discrimination of H. pluvialis strains. This technique differentiated the strains based on their geographical status. Academic Journals 2012 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/65146/1/65146.pdf Noroozi, Mostafa and Omar, Hishamuddin and Napis, Suhaimi and Hejazi, Mohammad Amin and Tan, Soon Guan (2012) Comparative biodiversity and effect of different media on growth and astaxanthin content of nine geographical strains of Haematococcus pluvialis. African Journal of Biotechnology, 11 (84). art. no. 6BBEBF328741. pp. 15049-15059. ISSN 1684–5315 http://www.academicjournals.org/journal/AJB/article-abstract/6BBEBF328741 10.5897/AJB12.773 |
| spellingShingle | Noroozi, Mostafa Omar, Hishamuddin Napis, Suhaimi Hejazi, Mohammad Amin Tan, Soon Guan Comparative biodiversity and effect of different media on growth and astaxanthin content of nine geographical strains of Haematococcus pluvialis |
| title | Comparative biodiversity and effect of different media on growth and astaxanthin content of nine geographical strains of Haematococcus pluvialis |
| title_full | Comparative biodiversity and effect of different media on growth and astaxanthin content of nine geographical strains of Haematococcus pluvialis |
| title_fullStr | Comparative biodiversity and effect of different media on growth and astaxanthin content of nine geographical strains of Haematococcus pluvialis |
| title_full_unstemmed | Comparative biodiversity and effect of different media on growth and astaxanthin content of nine geographical strains of Haematococcus pluvialis |
| title_short | Comparative biodiversity and effect of different media on growth and astaxanthin content of nine geographical strains of Haematococcus pluvialis |
| title_sort | comparative biodiversity and effect of different media on growth and astaxanthin content of nine geographical strains of haematococcus pluvialis |
| url | http://psasir.upm.edu.my/id/eprint/65146/1/65146.pdf |
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