Development and validation of high resolution melting assays for high-throughput screening of BDNF rs6265 and DAT1 rs40184
Introduction: One of the commonly used techniques for mutation screening is High Resolution Melting (HRM) analysis. HRM is a post PCR method that relies on the detection of the fluorescent signals acquired due to the release of DNA intercalated dyes upon the melting of dsDNA to ssDNA. The method is...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/66145/1/2018080309042108_MJMHS_Aug_2018.pdf |
| _version_ | 1796978241873903616 |
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| author | Faris, Asraa Md Yusof, Hadri Hadi Zainal Abidin, Shahidee Habib, Omar Cheah, Pike See Stanslas, Johnson Ibrahim, Normala Lye, Munn Sann Veerakumarasivam, Abhi Rosli, Rozita Ling, King Hwa |
| author_facet | Faris, Asraa Md Yusof, Hadri Hadi Zainal Abidin, Shahidee Habib, Omar Cheah, Pike See Stanslas, Johnson Ibrahim, Normala Lye, Munn Sann Veerakumarasivam, Abhi Rosli, Rozita Ling, King Hwa |
| author_sort | Faris, Asraa |
| collection | UPM |
| description | Introduction: One of the commonly used techniques for mutation screening is High Resolution Melting (HRM) analysis. HRM is a post PCR method that relies on the detection of the fluorescent signals acquired due to the release of DNA intercalated dyes upon the melting of dsDNA to ssDNA. The method is simple, inexpensive and does not require post PCR-handling, making it suitable for high throughput screening. Methods: This study aimed to develop and validate HRM technique for the screening of two disease-associated single nucleotide polymorphisms (SNPs) namely BDNF rs6265 and DAT1 rs40184 using a total of 30 gDNA samples. The obtained results were confirmed and validated by sequencing. Results: HRM analysis showed that the predicted genotypes of BDNF rs6265 and DAT1 rs40184 among all the gDNA samples were in 100% concordance with the sequencing results, making it an accurate and sensitive method for the detection of SNPs. Conclusions: The application of HRM can accurately determine the genotype of BDNF rs6265 and DAT1 rs40184 SNPs, making it a promising tool for rapid and high-throughput screening of targeted SNPs in a large population study. |
| first_indexed | 2024-03-06T09:51:45Z |
| format | Article |
| id | upm.eprints-66145 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:51:45Z |
| publishDate | 2018 |
| publisher | Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-661452019-02-11T03:56:16Z http://psasir.upm.edu.my/id/eprint/66145/ Development and validation of high resolution melting assays for high-throughput screening of BDNF rs6265 and DAT1 rs40184 Faris, Asraa Md Yusof, Hadri Hadi Zainal Abidin, Shahidee Habib, Omar Cheah, Pike See Stanslas, Johnson Ibrahim, Normala Lye, Munn Sann Veerakumarasivam, Abhi Rosli, Rozita Ling, King Hwa Introduction: One of the commonly used techniques for mutation screening is High Resolution Melting (HRM) analysis. HRM is a post PCR method that relies on the detection of the fluorescent signals acquired due to the release of DNA intercalated dyes upon the melting of dsDNA to ssDNA. The method is simple, inexpensive and does not require post PCR-handling, making it suitable for high throughput screening. Methods: This study aimed to develop and validate HRM technique for the screening of two disease-associated single nucleotide polymorphisms (SNPs) namely BDNF rs6265 and DAT1 rs40184 using a total of 30 gDNA samples. The obtained results were confirmed and validated by sequencing. Results: HRM analysis showed that the predicted genotypes of BDNF rs6265 and DAT1 rs40184 among all the gDNA samples were in 100% concordance with the sequencing results, making it an accurate and sensitive method for the detection of SNPs. Conclusions: The application of HRM can accurately determine the genotype of BDNF rs6265 and DAT1 rs40184 SNPs, making it a promising tool for rapid and high-throughput screening of targeted SNPs in a large population study. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/66145/1/2018080309042108_MJMHS_Aug_2018.pdf Faris, Asraa and Md Yusof, Hadri Hadi and Zainal Abidin, Shahidee and Habib, Omar and Cheah, Pike See and Stanslas, Johnson and Ibrahim, Normala and Lye, Munn Sann and Veerakumarasivam, Abhi and Rosli, Rozita and Ling, King Hwa (2018) Development and validation of high resolution melting assays for high-throughput screening of BDNF rs6265 and DAT1 rs40184. Malaysian Journal of Medicine and Health Sciences, 14 (SP1). pp. 64-71. ISSN 1675-8544; ESSN: 2636-9346 http://www.medic.upm.edu.my/upload/dokumen/2018080309042108_MJMHS_Aug_2018.pdf |
| spellingShingle | Faris, Asraa Md Yusof, Hadri Hadi Zainal Abidin, Shahidee Habib, Omar Cheah, Pike See Stanslas, Johnson Ibrahim, Normala Lye, Munn Sann Veerakumarasivam, Abhi Rosli, Rozita Ling, King Hwa Development and validation of high resolution melting assays for high-throughput screening of BDNF rs6265 and DAT1 rs40184 |
| title | Development and validation of high resolution melting assays for high-throughput screening of BDNF rs6265 and DAT1 rs40184 |
| title_full | Development and validation of high resolution melting assays for high-throughput screening of BDNF rs6265 and DAT1 rs40184 |
| title_fullStr | Development and validation of high resolution melting assays for high-throughput screening of BDNF rs6265 and DAT1 rs40184 |
| title_full_unstemmed | Development and validation of high resolution melting assays for high-throughput screening of BDNF rs6265 and DAT1 rs40184 |
| title_short | Development and validation of high resolution melting assays for high-throughput screening of BDNF rs6265 and DAT1 rs40184 |
| title_sort | development and validation of high resolution melting assays for high throughput screening of bdnf rs6265 and dat1 rs40184 |
| url | http://psasir.upm.edu.my/id/eprint/66145/1/2018080309042108_MJMHS_Aug_2018.pdf |
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