Acute phase proteins as early biomarkers in buffaloes infected with Pasteurella multocida type B: 2 and its immunogens (LPS and OMP)
Sudden death is usually the main finding in field animals during haemorrhagic septicaemia outbreaks in Malaysia due to the acute, fatal, and septicaemic disease of cattle and buffaloes. The aim of this study was to determine the acute phase responses in buffaloes infected with Pasteurella multocida...
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| Format: | Conference or Workshop Item |
| Language: | English |
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Faculty of Animal Husbandry, Brawijaya University
2017
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| Online Access: | http://psasir.upm.edu.my/id/eprint/66601/1/SAADC-2017-7.pdf |
| _version_ | 1825933167449604096 |
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| author | Lim, Eric Teik Chung Abdullah, Faez Firdaus Jesse Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Saad, Mohd Zamri Haron, Abd Wahid Mohd Lila, Mohd Azmi Saharee, Abdul Aziz |
| author_facet | Lim, Eric Teik Chung Abdullah, Faez Firdaus Jesse Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Saad, Mohd Zamri Haron, Abd Wahid Mohd Lila, Mohd Azmi Saharee, Abdul Aziz |
| author_sort | Lim, Eric Teik Chung |
| collection | UPM |
| description | Sudden death is usually the main finding in field animals during haemorrhagic septicaemia outbreaks in Malaysia due to the acute, fatal, and septicaemic disease of cattle and buffaloes. The aim of this study was to determine the acute phase responses in buffaloes infected with Pasteurella multocida type B: 2 and its immunogens lipopolysaccharide and outer membrane proteins. 21 buffalo heifers were divided into 7 treatment groups. Group 1 buffaloes were inoculated orally with 10 mL of sterile phosphate buffered saline (PBS) pH7 which act as negative control group. While Group 2 and 3 were inoculated subcutaneously and orally with 10 mL of 1012 colony forming unit (cfu) of Pasteurella multocida type B: 2 respectively. Group 4 and 5 buffaloes were inoculated with 10 mL of lipopolysaccharide broth intravenous and oral route respectively. Buffaloes in Group 6 and 7 were administered with 10 mL of outer membrane proteins broth subcutaneous and orally respectively. During the post infection period, all buffaloes were observed for clinical signs and clinical response for 21 days. Blood samples were also collected according to time interval for analyses of acute phase proteins concentration. All buffaloes from Group 1, 3, 4, 5, and 7 were able to survive throughout the stipulated experimental period of 21 days. Group 2 and 6 buffaloes were only able to survive for 12 hours and 3 days respectively. Buffaloes in all treatment groups of Pasteurella multocida type B: 2 and its immunogens lipopolysaccharide and outer membrane proteins showed significant increase in haptoglobin and serum amyloid A concentrations during early and late infection period respectively. There were significant different (p<0.05) in all treatment groups compared to control group. Buffaloes infected with Pasteurella multocida type B: 2 and its immunogens lipopolysaccharide and outer membrane proteins showed changes in both haptoglobin and serum amyloid A concentrations during the early and late infection period respectively. Information on this will play a significant role in understanding the acute phase response during haemorrhagic septicaemia outbreaks. |
| first_indexed | 2024-03-06T09:53:07Z |
| format | Conference or Workshop Item |
| id | upm.eprints-66601 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:53:07Z |
| publishDate | 2017 |
| publisher | Faculty of Animal Husbandry, Brawijaya University |
| record_format | dspace |
| spelling | upm.eprints-666012019-03-03T23:59:50Z http://psasir.upm.edu.my/id/eprint/66601/ Acute phase proteins as early biomarkers in buffaloes infected with Pasteurella multocida type B: 2 and its immunogens (LPS and OMP) Lim, Eric Teik Chung Abdullah, Faez Firdaus Jesse Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Saad, Mohd Zamri Haron, Abd Wahid Mohd Lila, Mohd Azmi Saharee, Abdul Aziz Sudden death is usually the main finding in field animals during haemorrhagic septicaemia outbreaks in Malaysia due to the acute, fatal, and septicaemic disease of cattle and buffaloes. The aim of this study was to determine the acute phase responses in buffaloes infected with Pasteurella multocida type B: 2 and its immunogens lipopolysaccharide and outer membrane proteins. 21 buffalo heifers were divided into 7 treatment groups. Group 1 buffaloes were inoculated orally with 10 mL of sterile phosphate buffered saline (PBS) pH7 which act as negative control group. While Group 2 and 3 were inoculated subcutaneously and orally with 10 mL of 1012 colony forming unit (cfu) of Pasteurella multocida type B: 2 respectively. Group 4 and 5 buffaloes were inoculated with 10 mL of lipopolysaccharide broth intravenous and oral route respectively. Buffaloes in Group 6 and 7 were administered with 10 mL of outer membrane proteins broth subcutaneous and orally respectively. During the post infection period, all buffaloes were observed for clinical signs and clinical response for 21 days. Blood samples were also collected according to time interval for analyses of acute phase proteins concentration. All buffaloes from Group 1, 3, 4, 5, and 7 were able to survive throughout the stipulated experimental period of 21 days. Group 2 and 6 buffaloes were only able to survive for 12 hours and 3 days respectively. Buffaloes in all treatment groups of Pasteurella multocida type B: 2 and its immunogens lipopolysaccharide and outer membrane proteins showed significant increase in haptoglobin and serum amyloid A concentrations during early and late infection period respectively. There were significant different (p<0.05) in all treatment groups compared to control group. Buffaloes infected with Pasteurella multocida type B: 2 and its immunogens lipopolysaccharide and outer membrane proteins showed changes in both haptoglobin and serum amyloid A concentrations during the early and late infection period respectively. Information on this will play a significant role in understanding the acute phase response during haemorrhagic septicaemia outbreaks. Faculty of Animal Husbandry, Brawijaya University 2017 Conference or Workshop Item PeerReviewed text en http://psasir.upm.edu.my/id/eprint/66601/1/SAADC-2017-7.pdf Lim, Eric Teik Chung and Abdullah, Faez Firdaus Jesse and Marza, Ali Dhiaa and Ibrahim, Hayder Hamzah and Saad, Mohd Zamri and Haron, Abd Wahid and Mohd Lila, Mohd Azmi and Saharee, Abdul Aziz (2017) Acute phase proteins as early biomarkers in buffaloes infected with Pasteurella multocida type B: 2 and its immunogens (LPS and OMP). In: 6th International Conference on Sustainable Animal Agriculture for Developing Countries (SAADC 2017), 16-19 Oct. 2017, The Singhasari Resort, Batu City, Indonesia. (pp. 473-477). |
| spellingShingle | Lim, Eric Teik Chung Abdullah, Faez Firdaus Jesse Marza, Ali Dhiaa Ibrahim, Hayder Hamzah Saad, Mohd Zamri Haron, Abd Wahid Mohd Lila, Mohd Azmi Saharee, Abdul Aziz Acute phase proteins as early biomarkers in buffaloes infected with Pasteurella multocida type B: 2 and its immunogens (LPS and OMP) |
| title | Acute phase proteins as early biomarkers in buffaloes infected with Pasteurella multocida type B: 2 and its immunogens (LPS and OMP) |
| title_full | Acute phase proteins as early biomarkers in buffaloes infected with Pasteurella multocida type B: 2 and its immunogens (LPS and OMP) |
| title_fullStr | Acute phase proteins as early biomarkers in buffaloes infected with Pasteurella multocida type B: 2 and its immunogens (LPS and OMP) |
| title_full_unstemmed | Acute phase proteins as early biomarkers in buffaloes infected with Pasteurella multocida type B: 2 and its immunogens (LPS and OMP) |
| title_short | Acute phase proteins as early biomarkers in buffaloes infected with Pasteurella multocida type B: 2 and its immunogens (LPS and OMP) |
| title_sort | acute phase proteins as early biomarkers in buffaloes infected with pasteurella multocida type b 2 and its immunogens lps and omp |
| url | http://psasir.upm.edu.my/id/eprint/66601/1/SAADC-2017-7.pdf |
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