Comparison of sugar content, mineral elements and antioxidant properties of Heterotrigona itama honey from suburban and forest in Malaysia
Introduction: In Malaysia, Heterotrigona itama sp. (stingless bee) industries start to grow rapidly since 2015 but the study on its health benefit is still lacking. This study was aimed to analyse and compare the sugar content, minerals and antioxidant properties of stingless bee honey collected fro...
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| Format: | Article |
| Language: | English |
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Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
2019
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| Online Access: | http://psasir.upm.edu.my/id/eprint/68396/1/2019041715510315_MJMHS_Vol_15_SP1.pdf |
| _version_ | 1796978711046651904 |
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| author | Zulhilmi Cheng, Mohamad Zulhafiz Shafiq Ismail, Maznah Chan, Kim Wei Ooi, Der Jiun Ismail, Norsharina Zawawi, Norhasnida Mohd Lila, Mohd Azmi Mohd Esa, Norhaizan |
| author_facet | Zulhilmi Cheng, Mohamad Zulhafiz Shafiq Ismail, Maznah Chan, Kim Wei Ooi, Der Jiun Ismail, Norsharina Zawawi, Norhasnida Mohd Lila, Mohd Azmi Mohd Esa, Norhaizan |
| author_sort | Zulhilmi Cheng, Mohamad Zulhafiz Shafiq |
| collection | UPM |
| description | Introduction: In Malaysia, Heterotrigona itama sp. (stingless bee) industries start to grow rapidly since 2015 but the study on its health benefit is still lacking. This study was aimed to analyse and compare the sugar content, minerals and antioxidant properties of stingless bee honey collected from forest and suburban area in Malaysia. Methods: Sugar content was determined by HPLC, minerals and heavy metals was determined by Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) and Atomic Absorption Spectrometer (AAS), total phenolic content (TPC) and total flavonoid content (TFC) by Folin-Ciocalteu and aluminium chloride colorimetry method, respectively. For determining the antioxidant activity of the samples, 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), 2,2’-azi-no-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assay were used. Results: Fructose, glucose and sucrose are found in all samples in range of 16.03-33.13 g/100g, 8.63-20.72 g/100g and 2.68-34.04 g/100g, respectively. Potassium and sodium were major minerals in all stingless bee honey with an average of 622.36 and 496.01 mg/kg, respectively. Sample from the forest (Sibu, F2) has the highest TPC and TFC with value 520.663±8.119μg GAE/g and 443.25±18.194μg RE/g, respectively. The higher antioxidant activities (DPPH, ABTS, FRAP) also found in samples collected from the forest (F2) with 602.15±12.7 μg TE/g, 575.18±9.38 μg TE/g and 641.36±42.11μg TE/g, respectively. Conclusion: All stingless bee honey studied shown a significant amount of important minerals and antioxidant properties with samples from forest clearly shown significantly higher TPC and TFC as well as the antioxidant activity than samples collected from the suburban area. |
| first_indexed | 2024-03-06T09:58:31Z |
| format | Article |
| id | upm.eprints-68396 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T09:58:31Z |
| publishDate | 2019 |
| publisher | Faculty of Medicine and Health Sciences, Universiti Putra Malaysia |
| record_format | dspace |
| spelling | upm.eprints-683962019-05-10T08:34:27Z http://psasir.upm.edu.my/id/eprint/68396/ Comparison of sugar content, mineral elements and antioxidant properties of Heterotrigona itama honey from suburban and forest in Malaysia Zulhilmi Cheng, Mohamad Zulhafiz Shafiq Ismail, Maznah Chan, Kim Wei Ooi, Der Jiun Ismail, Norsharina Zawawi, Norhasnida Mohd Lila, Mohd Azmi Mohd Esa, Norhaizan Introduction: In Malaysia, Heterotrigona itama sp. (stingless bee) industries start to grow rapidly since 2015 but the study on its health benefit is still lacking. This study was aimed to analyse and compare the sugar content, minerals and antioxidant properties of stingless bee honey collected from forest and suburban area in Malaysia. Methods: Sugar content was determined by HPLC, minerals and heavy metals was determined by Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES) and Atomic Absorption Spectrometer (AAS), total phenolic content (TPC) and total flavonoid content (TFC) by Folin-Ciocalteu and aluminium chloride colorimetry method, respectively. For determining the antioxidant activity of the samples, 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), 2,2’-azi-no-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and ferric reducing antioxidant power (FRAP) assay were used. Results: Fructose, glucose and sucrose are found in all samples in range of 16.03-33.13 g/100g, 8.63-20.72 g/100g and 2.68-34.04 g/100g, respectively. Potassium and sodium were major minerals in all stingless bee honey with an average of 622.36 and 496.01 mg/kg, respectively. Sample from the forest (Sibu, F2) has the highest TPC and TFC with value 520.663±8.119μg GAE/g and 443.25±18.194μg RE/g, respectively. The higher antioxidant activities (DPPH, ABTS, FRAP) also found in samples collected from the forest (F2) with 602.15±12.7 μg TE/g, 575.18±9.38 μg TE/g and 641.36±42.11μg TE/g, respectively. Conclusion: All stingless bee honey studied shown a significant amount of important minerals and antioxidant properties with samples from forest clearly shown significantly higher TPC and TFC as well as the antioxidant activity than samples collected from the suburban area. Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 2019 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/68396/1/2019041715510315_MJMHS_Vol_15_SP1.pdf Zulhilmi Cheng, Mohamad Zulhafiz Shafiq and Ismail, Maznah and Chan, Kim Wei and Ooi, Der Jiun and Ismail, Norsharina and Zawawi, Norhasnida and Mohd Lila, Mohd Azmi and Mohd Esa, Norhaizan (2019) Comparison of sugar content, mineral elements and antioxidant properties of Heterotrigona itama honey from suburban and forest in Malaysia. Malaysian Journal of Medicine and Health Sciences, 15 (SP1). pp. 104-112. ISSN 1675-8544; ESSN: 2636-9346 http://www.medic.upm.edu.my/upload/dokumen/2019041715510315_MJMHS_Vol_15_SP1.pdf |
| spellingShingle | Zulhilmi Cheng, Mohamad Zulhafiz Shafiq Ismail, Maznah Chan, Kim Wei Ooi, Der Jiun Ismail, Norsharina Zawawi, Norhasnida Mohd Lila, Mohd Azmi Mohd Esa, Norhaizan Comparison of sugar content, mineral elements and antioxidant properties of Heterotrigona itama honey from suburban and forest in Malaysia |
| title | Comparison of sugar content, mineral elements and antioxidant properties of Heterotrigona itama honey from suburban and forest in Malaysia |
| title_full | Comparison of sugar content, mineral elements and antioxidant properties of Heterotrigona itama honey from suburban and forest in Malaysia |
| title_fullStr | Comparison of sugar content, mineral elements and antioxidant properties of Heterotrigona itama honey from suburban and forest in Malaysia |
| title_full_unstemmed | Comparison of sugar content, mineral elements and antioxidant properties of Heterotrigona itama honey from suburban and forest in Malaysia |
| title_short | Comparison of sugar content, mineral elements and antioxidant properties of Heterotrigona itama honey from suburban and forest in Malaysia |
| title_sort | comparison of sugar content mineral elements and antioxidant properties of heterotrigona itama honey from suburban and forest in malaysia |
| url | http://psasir.upm.edu.my/id/eprint/68396/1/2019041715510315_MJMHS_Vol_15_SP1.pdf |
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