Effects of xylanase and cellulase supplementation on early growing stage, caecal bacteria population and fermentation profiles of broiler fed with palm kernel meal
The optimum inclusion of palm kernel meal in broiler diets is reported about 20% without having any detrimental effect on the performance and health of broilers. The addition of enzyme in palm kernel mealdiet for broilers isnecessary in promoting growth performances of the broilers through increa...
| 主要作者: | |
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| 格式: | Thesis |
| 语言: | English |
| 出版: |
2014
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| 主题: | |
| 在线阅读: | http://psasir.upm.edu.my/id/eprint/70374/1/FP%202014%2081%20-%20IR.pdf |
| _version_ | 1825933906674712576 |
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| author | Ahmad, Sharmila |
| author_facet | Ahmad, Sharmila |
| author_sort | Ahmad, Sharmila |
| collection | UPM |
| description | The optimum inclusion of palm kernel meal in broiler diets is reported about 20%
without having any detrimental effect on the performance and health of broilers. The
addition of enzyme in palm kernel mealdiet for broilers isnecessary in promoting
growth performances of the broilers through increase in feed efficiency and
digestibility. Since, previous study only focusing on their effect on growth
performance; therefore, the present study was conducted to study the effect of
enzyme supplementation in palm kernel mealdiet on caecal fermentation profiles,
early growth performances and caecal bacteria population of broilers. The in-vitro
analysis was carried out by incubating the formulated diets with caecal digesta of
broilers for 72 hours at 39±5oC. The gas production and end products of the
fermentation were examined. The gas production rate of unsupplemented and
enzyme-supplemented palm kernel meal diet observed was similar in trends.
However, it was found that the amount of acetate was significantly (P<0.05) reduced
with enzyme supplementation, whilst the in-vitro dry matter degradability of both,
starter and finisher diet were improved (P<0.05), with more outstanding result
observedfollowing xylanase supplementation at 11.09 and 17.59%, respectively.In
the feeding trial, 75 broiler chicks were randomly allocated to three dietary treatment
groups receiving diet containing 20% of palm kernel meal with or without xylanase
and cellulase supplementation. The individual body weight and feed intakewere
recorded weekly, and at day 35, broilers were slaughtered for ceacal digesta
collection. Both enzyme addition did not improve the average daily gain of broiler at
starter phase (1- 21 days), but had improved at grower phase (22-35 days), where
broilers fed with xylanase supplementation had improved their body weight by 7%
compared to control diet (61.98 g/bird). The similar result was also observed in the
final body weight of broilers, where xylanase-supplemented group was the highest
followed by control and cellulase-supplemented group which values is 1495.2,
1436.8 and 1367.8 kg, respectively. In the enumeration of caecal bacteria population,
lactic acid bacteria and Salmonella spp. population were reduced, by 4.1 and 1.9% in xylanase supplemented group, and 8.3 and 7.9%in cellulase supplemented group,
respectively. In contrast, the caecal bacteria population quantified using real-time
PCR analysis revealed that enzyme supplementation had reduced the number of total
bacteria, lactic acid bacteria, enterococcus, enterobacteria, Escherichia coli,
bifidobacteria and Salmonellaspp., respectively compared to unsupplemented palm
kernel meal diet. Apart from that, significant negative correlation (P<0.01) was
observed between the population of coliform bacteria (-0.97) and Salmonella spp. (-
0.84) with n-butyrate in caecal digesta of broilers. The current data show that nbutyrate
have a toxic effect on coliform bacteria and Salmonella spp. In conclusion,
enzyme supplementation in 20% palm kernel meal diet was only effective in
improving the body weight of broilers at growing phase but not in the starter phase.
So, reducing the amount of palm kernel meal in the starter broiler diet is
recommended; since, body weight of broilers fed 20% palm kernel meal diet was not
improved, even with enzyme addition. |
| first_indexed | 2024-03-06T10:04:30Z |
| format | Thesis |
| id | upm.eprints-70374 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:04:30Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | upm.eprints-703742019-10-30T08:40:06Z http://psasir.upm.edu.my/id/eprint/70374/ Effects of xylanase and cellulase supplementation on early growing stage, caecal bacteria population and fermentation profiles of broiler fed with palm kernel meal Ahmad, Sharmila The optimum inclusion of palm kernel meal in broiler diets is reported about 20% without having any detrimental effect on the performance and health of broilers. The addition of enzyme in palm kernel mealdiet for broilers isnecessary in promoting growth performances of the broilers through increase in feed efficiency and digestibility. Since, previous study only focusing on their effect on growth performance; therefore, the present study was conducted to study the effect of enzyme supplementation in palm kernel mealdiet on caecal fermentation profiles, early growth performances and caecal bacteria population of broilers. The in-vitro analysis was carried out by incubating the formulated diets with caecal digesta of broilers for 72 hours at 39±5oC. The gas production and end products of the fermentation were examined. The gas production rate of unsupplemented and enzyme-supplemented palm kernel meal diet observed was similar in trends. However, it was found that the amount of acetate was significantly (P<0.05) reduced with enzyme supplementation, whilst the in-vitro dry matter degradability of both, starter and finisher diet were improved (P<0.05), with more outstanding result observedfollowing xylanase supplementation at 11.09 and 17.59%, respectively.In the feeding trial, 75 broiler chicks were randomly allocated to three dietary treatment groups receiving diet containing 20% of palm kernel meal with or without xylanase and cellulase supplementation. The individual body weight and feed intakewere recorded weekly, and at day 35, broilers were slaughtered for ceacal digesta collection. Both enzyme addition did not improve the average daily gain of broiler at starter phase (1- 21 days), but had improved at grower phase (22-35 days), where broilers fed with xylanase supplementation had improved their body weight by 7% compared to control diet (61.98 g/bird). The similar result was also observed in the final body weight of broilers, where xylanase-supplemented group was the highest followed by control and cellulase-supplemented group which values is 1495.2, 1436.8 and 1367.8 kg, respectively. In the enumeration of caecal bacteria population, lactic acid bacteria and Salmonella spp. population were reduced, by 4.1 and 1.9% in xylanase supplemented group, and 8.3 and 7.9%in cellulase supplemented group, respectively. In contrast, the caecal bacteria population quantified using real-time PCR analysis revealed that enzyme supplementation had reduced the number of total bacteria, lactic acid bacteria, enterococcus, enterobacteria, Escherichia coli, bifidobacteria and Salmonellaspp., respectively compared to unsupplemented palm kernel meal diet. Apart from that, significant negative correlation (P<0.01) was observed between the population of coliform bacteria (-0.97) and Salmonella spp. (- 0.84) with n-butyrate in caecal digesta of broilers. The current data show that nbutyrate have a toxic effect on coliform bacteria and Salmonella spp. In conclusion, enzyme supplementation in 20% palm kernel meal diet was only effective in improving the body weight of broilers at growing phase but not in the starter phase. So, reducing the amount of palm kernel meal in the starter broiler diet is recommended; since, body weight of broilers fed 20% palm kernel meal diet was not improved, even with enzyme addition. 2014-12 Thesis NonPeerReviewed text en http://psasir.upm.edu.my/id/eprint/70374/1/FP%202014%2081%20-%20IR.pdf Ahmad, Sharmila (2014) Effects of xylanase and cellulase supplementation on early growing stage, caecal bacteria population and fermentation profiles of broiler fed with palm kernel meal. Masters thesis, Universiti Putra Malaysia. Palm products Poultry industry |
| spellingShingle | Palm products Poultry industry Ahmad, Sharmila Effects of xylanase and cellulase supplementation on early growing stage, caecal bacteria population and fermentation profiles of broiler fed with palm kernel meal |
| title | Effects of xylanase and cellulase supplementation on early growing stage, caecal bacteria population and fermentation profiles of broiler fed with palm kernel meal |
| title_full | Effects of xylanase and cellulase supplementation on early growing stage, caecal bacteria population and fermentation profiles of broiler fed with palm kernel meal |
| title_fullStr | Effects of xylanase and cellulase supplementation on early growing stage, caecal bacteria population and fermentation profiles of broiler fed with palm kernel meal |
| title_full_unstemmed | Effects of xylanase and cellulase supplementation on early growing stage, caecal bacteria population and fermentation profiles of broiler fed with palm kernel meal |
| title_short | Effects of xylanase and cellulase supplementation on early growing stage, caecal bacteria population and fermentation profiles of broiler fed with palm kernel meal |
| title_sort | effects of xylanase and cellulase supplementation on early growing stage caecal bacteria population and fermentation profiles of broiler fed with palm kernel meal |
| topic | Palm products Poultry industry |
| url | http://psasir.upm.edu.my/id/eprint/70374/1/FP%202014%2081%20-%20IR.pdf |
| work_keys_str_mv | AT ahmadsharmila effectsofxylanaseandcellulasesupplementationonearlygrowingstagecaecalbacteriapopulationandfermentationprofilesofbroilerfedwithpalmkernelmeal |