High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5
An organic solvent-tolerant S5 lipase was purified by affinity chromatography and anion exchange chromatography. The molecular mass of the lipase was estimated to be 60 kDa with 387 purification fold. The optimal temperature and pH were 45 °C and 9.0, respectively. The purified lipase was stable at...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7071/1/High.pdf |
| _version_ | 1796967249702027264 |
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| author | Raja Abdul Rahman, Raja Noor Zaliha Baharum, Syarul Nataqain Basri, Mahiran Salleh, Abu Bakar |
| author_facet | Raja Abdul Rahman, Raja Noor Zaliha Baharum, Syarul Nataqain Basri, Mahiran Salleh, Abu Bakar |
| author_sort | Raja Abdul Rahman, Raja Noor Zaliha |
| collection | UPM |
| description | An organic solvent-tolerant S5 lipase was purified by affinity chromatography and anion exchange chromatography. The molecular mass of the lipase was estimated to be 60 kDa with 387 purification fold. The optimal temperature and pH were 45 °C and 9.0, respectively. The purified lipase was stable at 45 °C and pH 6–9. It exhibited the highest stability in the presence of various organic solvents such as n-dodecane, 1-pentanol, and toluene. Ca2+ and Mg2+ stimulated lipase activity, whereas EDTA had no effect on its activity. The S5 lipase exhibited the highest activity in the presence of palm oil as a natural oil and triolein as a synthetic triglyceride. It showed random positional specificity on the thin-layer chromatography. |
| first_indexed | 2024-03-06T07:11:09Z |
| format | Article |
| id | upm.eprints-7071 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:11:09Z |
| publishDate | 2005 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-70712016-09-28T04:49:43Z http://psasir.upm.edu.my/id/eprint/7071/ High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5 Raja Abdul Rahman, Raja Noor Zaliha Baharum, Syarul Nataqain Basri, Mahiran Salleh, Abu Bakar An organic solvent-tolerant S5 lipase was purified by affinity chromatography and anion exchange chromatography. The molecular mass of the lipase was estimated to be 60 kDa with 387 purification fold. The optimal temperature and pH were 45 °C and 9.0, respectively. The purified lipase was stable at 45 °C and pH 6–9. It exhibited the highest stability in the presence of various organic solvents such as n-dodecane, 1-pentanol, and toluene. Ca2+ and Mg2+ stimulated lipase activity, whereas EDTA had no effect on its activity. The S5 lipase exhibited the highest activity in the presence of palm oil as a natural oil and triolein as a synthetic triglyceride. It showed random positional specificity on the thin-layer chromatography. Elsevier 2005-06-15 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7071/1/High.pdf Raja Abdul Rahman, Raja Noor Zaliha and Baharum, Syarul Nataqain and Basri, Mahiran and Salleh, Abu Bakar (2005) High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5. Analytical Biochemistry, 341 (2). pp. 267-274. ISSN 0003-2697; ESSN: 1096-0309 http://www.sciencedirect.com/science/article/pii/S0003269705001752 10.1016/j.ab.2005.03.006 |
| spellingShingle | Raja Abdul Rahman, Raja Noor Zaliha Baharum, Syarul Nataqain Basri, Mahiran Salleh, Abu Bakar High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5 |
| title | High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5 |
| title_full | High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5 |
| title_fullStr | High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5 |
| title_full_unstemmed | High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5 |
| title_short | High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5 |
| title_sort | high yield purification of an organic solvent tolerant lipase from pseudomonas sp strain s5 |
| url | http://psasir.upm.edu.my/id/eprint/7071/1/High.pdf |
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