High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5
An organic solvent-tolerant S5 lipase was purified by affinity chromatography and anion exchange chromatography. The molecular mass of the lipase was estimated to be 60 kDa with 387 purification fold. The optimal temperature and pH were 45 °C and 9.0, respectively. The purified lipase was stable at...
Main Authors: | Raja Abdul Rahman, Raja Noor Zaliha, Baharum, Syarul Nataqain, Basri, Mahiran, Salleh, Abu Bakar |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2005
|
Online Access: | http://psasir.upm.edu.my/id/eprint/7071/1/High.pdf |
Similar Items
-
Chaperone-dependent gene expression of organic solvent-tolerant lipase from Pseudomonas aeruginosa strain S5
by: Baharum, Syarul Nataqain, et al.
Published: (2010) -
Organic solvent tolerant lipases
by: Baharum, Syarul Nataqain, et al.
Published: (2006) -
Molecular expression of novel organic solvent tolerant lipases
by: Baharum, Syarul Nataqain, et al.
Published: (2013) -
Production, Characterization And Expression Of An Organic Solvent Tolerant Lipase From Pseudomonas Aeruginosa S5s5
by: Baharum, Syarul Nataqain
Published: (2005) -
Characterization and solvent stability of strep-tagged purified recombinant lipase from thermostable and solvent tolerant Bacillus sp. strain 42.
by: Tengku Abdul Hamid, Tengku Haziyamin, et al.
Published: (2009)