Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K
The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 °C with 37 °C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7075/1/Physical%20factors%20affecting%20the%20production%20of%20organic%20solvent.pdf |
| _version_ | 1796967250498945024 |
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| author | Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar |
| author_facet | Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar |
| author_sort | Raja Abdul Rahman, Raja Noor Zaliha |
| collection | UPM |
| description | The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 °C with 37 °C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0–10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media. |
| first_indexed | 2024-03-06T07:11:10Z |
| format | Article |
| id | upm.eprints-7075 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:11:10Z |
| publishDate | 2005 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-70752016-09-28T07:03:33Z http://psasir.upm.edu.my/id/eprint/7075/ Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 °C with 37 °C being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0–10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media. Elsevier 2005-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7075/1/Physical%20factors%20affecting%20the%20production%20of%20organic%20solvent.pdf Raja Abdul Rahman, Raja Noor Zaliha and Lee, Poh Geok and Basri, Mahiran and Salleh, Abu Bakar (2005) Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K. Bioresource Technology, 96 (4). pp. 429-436. ISSN 0960-8524; ESSN: 1873-2976 http://www.sciencedirect.com/science/article/pii/S0960852404002238 10.1016/j.biortech.2004.06.012 |
| spellingShingle | Raja Abdul Rahman, Raja Noor Zaliha Lee, Poh Geok Basri, Mahiran Salleh, Abu Bakar Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K |
| title | Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K |
| title_full | Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K |
| title_fullStr | Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K |
| title_full_unstemmed | Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K |
| title_short | Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K |
| title_sort | physical factors affecting the production of organic solvent tolerant protease by pseudomonas aeruginosa strain k |
| url | http://psasir.upm.edu.my/id/eprint/7075/1/Physical%20factors%20affecting%20the%20production%20of%20organic%20solvent.pdf |
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