Optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium Bacillus cereus strain 146
Optimization of the growth conditions for maximum growth rate and alkaline protease production was carried out using the newly isolated mesophilic bacterium Bacillus cereus strain 146. The bacterium produced protease at maximum rate after 48 h of incubation at 37°C with agitation speed of 170 rpm an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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INSInet Publication
2005
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7088/1/Optimization%20of%20environmental%20and%20nutritional%20conditions%20for%20the%20production%20of%20alkaline%20protease%20by%20a%20newly%20isolated%20bacterium%20Bacillus%20cereus%20strain%20146.pdf |
| _version_ | 1825944139269668864 |
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| author | Shafee, Norazizah Aris, Sayangku Norariati Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Salleh, Abu Bakar |
| author_facet | Shafee, Norazizah Aris, Sayangku Norariati Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Salleh, Abu Bakar |
| author_sort | Shafee, Norazizah |
| collection | UPM |
| description | Optimization of the growth conditions for maximum growth rate and alkaline protease production was carried out using the newly isolated mesophilic bacterium Bacillus cereus strain 146. The bacterium produced protease at maximum rate after 48 h of incubation at 37°C with agitation speed of 170 rpm and 4% (v/v) starter culture. The best carbon and organic nitrogen sources for this bacterium were glucose and beef extract, respectively. While the most effective inorganic nitrogen sources were urea and lysine. Supplementation of the culture medium with Mn2+ improved the protease production substantially. Under these conditions B. cereus strain 146 was found to produce alkaline protease at a maximum rate of approximately 2.0 μg/mL/min. |
| first_indexed | 2024-03-06T07:11:12Z |
| format | Article |
| id | upm.eprints-7088 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T07:11:12Z |
| publishDate | 2005 |
| publisher | INSInet Publication |
| record_format | dspace |
| spelling | upm.eprints-70882016-09-26T08:21:08Z http://psasir.upm.edu.my/id/eprint/7088/ Optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium Bacillus cereus strain 146 Shafee, Norazizah Aris, Sayangku Norariati Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Salleh, Abu Bakar Optimization of the growth conditions for maximum growth rate and alkaline protease production was carried out using the newly isolated mesophilic bacterium Bacillus cereus strain 146. The bacterium produced protease at maximum rate after 48 h of incubation at 37°C with agitation speed of 170 rpm and 4% (v/v) starter culture. The best carbon and organic nitrogen sources for this bacterium were glucose and beef extract, respectively. While the most effective inorganic nitrogen sources were urea and lysine. Supplementation of the culture medium with Mn2+ improved the protease production substantially. Under these conditions B. cereus strain 146 was found to produce alkaline protease at a maximum rate of approximately 2.0 μg/mL/min. INSInet Publication 2005 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7088/1/Optimization%20of%20environmental%20and%20nutritional%20conditions%20for%20the%20production%20of%20alkaline%20protease%20by%20a%20newly%20isolated%20bacterium%20Bacillus%20cereus%20strain%20146.pdf Shafee, Norazizah and Aris, Sayangku Norariati and Raja Abdul Rahman, Raja Noor Zaliha and Basri, Mahiran and Salleh, Abu Bakar (2005) Optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium Bacillus cereus strain 146. Journal of Applied Sciences Research, 1 (1). pp. 1-8. ISSN 1816-157X http://www.aensiweb.com/old/jasr/jasr_august_2005.html |
| spellingShingle | Shafee, Norazizah Aris, Sayangku Norariati Raja Abdul Rahman, Raja Noor Zaliha Basri, Mahiran Salleh, Abu Bakar Optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium Bacillus cereus strain 146 |
| title | Optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium Bacillus cereus strain 146 |
| title_full | Optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium Bacillus cereus strain 146 |
| title_fullStr | Optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium Bacillus cereus strain 146 |
| title_full_unstemmed | Optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium Bacillus cereus strain 146 |
| title_short | Optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium Bacillus cereus strain 146 |
| title_sort | optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium bacillus cereus strain 146 |
| url | http://psasir.upm.edu.my/id/eprint/7088/1/Optimization%20of%20environmental%20and%20nutritional%20conditions%20for%20the%20production%20of%20alkaline%20protease%20by%20a%20newly%20isolated%20bacterium%20Bacillus%20cereus%20strain%20146.pdf |
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