Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis
A total of 78 samples of street foods from different states in Malaysia were examined for the presence of Klebsiella pneumonia. K. pneumonia contamination was recorded in 32% of the samples examined. The frequency of the K. pneumonia isolates containing plasmids was shown to be 48%. Susceptibility t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Sprog: | English English |
| Udgivet: |
Elsevier
2007
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| Online adgang: | http://psasir.upm.edu.my/id/eprint/7155/1/Incidence%20of%20Klebsiella%20pneumonia%20in%20street%20foods%20sold%20in%20Malaysia%20and%20their%20characterization%20by%20antibiotic%20resistance.pdf |
| Summary: | A total of 78 samples of street foods from different states in Malaysia were examined for the presence of Klebsiella pneumonia. K. pneumonia contamination was recorded in 32% of the samples examined. The frequency of the K. pneumonia isolates containing plasmids was shown to be 48%. Susceptibility testing showed that all isolates were highly resistant to ampicillin (100%), erythromycin (100%), rifampicin (100%), streptomycin (96%) and sulfamethoxazole (80%), but susceptible to chloramphenicol. RAPD proved to be the most effective technique in discriminating the K. pneumonia isolates since dendrogram constructed using the combination of 4 primers could differentiate 25 isolates of K. pneumonia to 25 strains. This is the first report that revealed the occurrence of K. pneumonia in street foods sold in Malaysia. |
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