Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis
A total of 78 samples of street foods from different states in Malaysia were examined for the presence of Klebsiella pneumonia. K. pneumonia contamination was recorded in 32% of the samples examined. The frequency of the K. pneumonia isolates containing plasmids was shown to be 48%. Susceptibility t...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
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Elsevier
2007
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7155/1/Incidence%20of%20Klebsiella%20pneumonia%20in%20street%20foods%20sold%20in%20Malaysia%20and%20their%20characterization%20by%20antibiotic%20resistance.pdf |
| _version_ | 1796967265787183104 |
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| author | Yuli, Haryani Awang Saleh, Noorzaleha Abu Bakar, Fatimah Mohamed Alitheen, Noorjahan Banu Patrick, Guda Benjamin Abdul Talib, Shamsinar Ahmad Suhaimi, Laila Rabaah Radu, Son |
| author_facet | Yuli, Haryani Awang Saleh, Noorzaleha Abu Bakar, Fatimah Mohamed Alitheen, Noorjahan Banu Patrick, Guda Benjamin Abdul Talib, Shamsinar Ahmad Suhaimi, Laila Rabaah Radu, Son |
| author_sort | Yuli, Haryani |
| collection | UPM |
| description | A total of 78 samples of street foods from different states in Malaysia were examined for the presence of Klebsiella pneumonia. K. pneumonia contamination was recorded in 32% of the samples examined. The frequency of the K. pneumonia isolates containing plasmids was shown to be 48%. Susceptibility testing showed that all isolates were highly resistant to ampicillin (100%), erythromycin (100%), rifampicin (100%), streptomycin (96%) and sulfamethoxazole (80%), but susceptible to chloramphenicol. RAPD proved to be the most effective technique in discriminating the K. pneumonia isolates since dendrogram constructed using the combination of 4 primers could differentiate 25 isolates of K. pneumonia to 25 strains. This is the first report that revealed the occurrence of K. pneumonia in street foods sold in Malaysia. |
| first_indexed | 2024-03-06T07:11:24Z |
| format | Article |
| id | upm.eprints-7155 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:11:24Z |
| publishDate | 2007 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-71552015-11-11T03:06:28Z http://psasir.upm.edu.my/id/eprint/7155/ Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis Yuli, Haryani Awang Saleh, Noorzaleha Abu Bakar, Fatimah Mohamed Alitheen, Noorjahan Banu Patrick, Guda Benjamin Abdul Talib, Shamsinar Ahmad Suhaimi, Laila Rabaah Radu, Son A total of 78 samples of street foods from different states in Malaysia were examined for the presence of Klebsiella pneumonia. K. pneumonia contamination was recorded in 32% of the samples examined. The frequency of the K. pneumonia isolates containing plasmids was shown to be 48%. Susceptibility testing showed that all isolates were highly resistant to ampicillin (100%), erythromycin (100%), rifampicin (100%), streptomycin (96%) and sulfamethoxazole (80%), but susceptible to chloramphenicol. RAPD proved to be the most effective technique in discriminating the K. pneumonia isolates since dendrogram constructed using the combination of 4 primers could differentiate 25 isolates of K. pneumonia to 25 strains. This is the first report that revealed the occurrence of K. pneumonia in street foods sold in Malaysia. Elsevier 2007 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7155/1/Incidence%20of%20Klebsiella%20pneumonia%20in%20street%20foods%20sold%20in%20Malaysia%20and%20their%20characterization%20by%20antibiotic%20resistance.pdf Yuli, Haryani and Awang Saleh, Noorzaleha and Abu Bakar, Fatimah and Mohamed Alitheen, Noorjahan Banu and Patrick, Guda Benjamin and Abdul Talib, Shamsinar and Ahmad Suhaimi, Laila Rabaah and Radu, Son (2007) Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis. Food Control, 18 (7). pp. 847-853. ISSN 0956-7135 http://dx.doi.org/10.1016/j.foodcont.2006.04.009 10.1016/j.foodcont.2006.04.009 English |
| spellingShingle | Yuli, Haryani Awang Saleh, Noorzaleha Abu Bakar, Fatimah Mohamed Alitheen, Noorjahan Banu Patrick, Guda Benjamin Abdul Talib, Shamsinar Ahmad Suhaimi, Laila Rabaah Radu, Son Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis |
| title | Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis |
| title_full | Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis |
| title_fullStr | Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis |
| title_full_unstemmed | Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis |
| title_short | Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis |
| title_sort | incidence of klebsiella pneumonia in street foods sold in malaysia and their characterization by antibiotic resistance plasmid profiling and rapd pcr analysis |
| url | http://psasir.upm.edu.my/id/eprint/7155/1/Incidence%20of%20Klebsiella%20pneumonia%20in%20street%20foods%20sold%20in%20Malaysia%20and%20their%20characterization%20by%20antibiotic%20resistance.pdf |
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