Development of a Capacitance-Based Biosensor for the Determination of Histamine Concentration
Histamine level has been suggested as a rapid fish spoilage indicator. Besides that, a biosensor device with low cost, simple and portable is an advantage for fish freshness monitoring. Therefore, a histamine biosensor device was developed using the enzyme diamine oxidase (DAO) and a capacitance...
| Main Author: | |
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| Format: | Thesis |
| Language: | English English |
| Published: |
2009
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| Online Access: | http://psasir.upm.edu.my/id/eprint/7209/1/IB_2009_1a.pdf |
| _version_ | 1825944163134210048 |
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| author | Wasoh @ Mohamad Isa, Helmi |
| author_facet | Wasoh @ Mohamad Isa, Helmi |
| author_sort | Wasoh @ Mohamad Isa, Helmi |
| collection | UPM |
| description | Histamine level has been suggested as a rapid fish spoilage indicator. Besides that, a
biosensor device with low cost, simple and portable is an advantage for fish freshness
monitoring. Therefore, a histamine biosensor device was developed using the enzyme
diamine oxidase (DAO) and a capacitance (C) concept. A capacitor with two plates
system gives linear relationship between frequency (f) and histamine concentration. It has
been proven that the relationship between f, dielectric constant (k) and histamine could be
used to develop a histamine biosensor. The behavior of histamine reaction can be
monitored in the presence of free enzyme in solution. Based on this relationship,
immobilized enzyme electrode was developed by immobilizing 1.0 mg DAO/L in the
ratio of DAO:polymer= 1:1. Good sensor response could be observed when the shape of
the electrode was in a row and the electrode material was made from copper, with a
distance between electrode, d=2.5 mm. By using this device, a linear range of histamine
from 20 to 100 ppm was obtained (R2=0.9897) with good correlation between
biosensor method and HPLC (R2 = 0.9998). For the determination of histamine in prawn tissues, the recovery obtained was 98.13% after spiking with 150 ppm
histamine and 98.79% after spiking with 200 ppm histamine (average RSD, 1.16-5.67%).
To complete the device, electronic reader was developed comprises PIC (peripheral
integrated circuit) microcontroller, LCD (light crystal display), capacitor-resistor circuit
and computer-programming in C code (installed in PIC). By using astable operation of
capacitor-resistor circuit, a difference sensor response was generated from difference
histamine concentration. During histamine reaction, the physicochemical changes was
converted into electrical signal and translated in histamine concentration as part per
million (ppm). PIC is very useful in modifying the writing process of computerprogramming
due to its capability and easily reprogrammed.
For the basic test (without enzyme), the device (with electronic reader) gave different (f)
values for histamine (50-300 ppm, RSD≤2.01%). After the reader was developed
successfully, enzyme electrode was designed with the dimension of copper material at
d=2.0 mm and area, A=2.5x10 mm. By immobilizing enzyme onto this electrode, the
device showed linear response to histamine concentrations (25 to 100 ppm, R2=0.998,
RSD≤2.74%). The biosensor response was still 80% of the initial value after 10 days of
storage (4oC). The histamine biosensor exhibited reproducibility characteristic with RSD
value equals to 8.88% (n=4). This device can be used up to 30 times without a major
change in sensor response (11.76±3.41 Hz/s). Experiment with prawn tissues shows that
the performance of histamine biosensor device is comparable to HPLC with R2=0.9895.
The histamine biosensor is a promising device for on site screening of fish freshness |
| first_indexed | 2024-03-06T07:11:34Z |
| format | Thesis |
| id | upm.eprints-7209 |
| institution | Universiti Putra Malaysia |
| language | English English |
| last_indexed | 2024-03-06T07:11:34Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | upm.eprints-72092013-05-27T07:34:05Z http://psasir.upm.edu.my/id/eprint/7209/ Development of a Capacitance-Based Biosensor for the Determination of Histamine Concentration Wasoh @ Mohamad Isa, Helmi Histamine level has been suggested as a rapid fish spoilage indicator. Besides that, a biosensor device with low cost, simple and portable is an advantage for fish freshness monitoring. Therefore, a histamine biosensor device was developed using the enzyme diamine oxidase (DAO) and a capacitance (C) concept. A capacitor with two plates system gives linear relationship between frequency (f) and histamine concentration. It has been proven that the relationship between f, dielectric constant (k) and histamine could be used to develop a histamine biosensor. The behavior of histamine reaction can be monitored in the presence of free enzyme in solution. Based on this relationship, immobilized enzyme electrode was developed by immobilizing 1.0 mg DAO/L in the ratio of DAO:polymer= 1:1. Good sensor response could be observed when the shape of the electrode was in a row and the electrode material was made from copper, with a distance between electrode, d=2.5 mm. By using this device, a linear range of histamine from 20 to 100 ppm was obtained (R2=0.9897) with good correlation between biosensor method and HPLC (R2 = 0.9998). For the determination of histamine in prawn tissues, the recovery obtained was 98.13% after spiking with 150 ppm histamine and 98.79% after spiking with 200 ppm histamine (average RSD, 1.16-5.67%). To complete the device, electronic reader was developed comprises PIC (peripheral integrated circuit) microcontroller, LCD (light crystal display), capacitor-resistor circuit and computer-programming in C code (installed in PIC). By using astable operation of capacitor-resistor circuit, a difference sensor response was generated from difference histamine concentration. During histamine reaction, the physicochemical changes was converted into electrical signal and translated in histamine concentration as part per million (ppm). PIC is very useful in modifying the writing process of computerprogramming due to its capability and easily reprogrammed. For the basic test (without enzyme), the device (with electronic reader) gave different (f) values for histamine (50-300 ppm, RSD≤2.01%). After the reader was developed successfully, enzyme electrode was designed with the dimension of copper material at d=2.0 mm and area, A=2.5x10 mm. By immobilizing enzyme onto this electrode, the device showed linear response to histamine concentrations (25 to 100 ppm, R2=0.998, RSD≤2.74%). The biosensor response was still 80% of the initial value after 10 days of storage (4oC). The histamine biosensor exhibited reproducibility characteristic with RSD value equals to 8.88% (n=4). This device can be used up to 30 times without a major change in sensor response (11.76±3.41 Hz/s). Experiment with prawn tissues shows that the performance of histamine biosensor device is comparable to HPLC with R2=0.9895. The histamine biosensor is a promising device for on site screening of fish freshness 2009-03 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/7209/1/IB_2009_1a.pdf Wasoh @ Mohamad Isa, Helmi (2009) Development of a Capacitance-Based Biosensor for the Determination of Histamine Concentration. PhD thesis, Universiti Putra Malaysia. Histamine Biosensors English |
| spellingShingle | Histamine Biosensors Wasoh @ Mohamad Isa, Helmi Development of a Capacitance-Based Biosensor for the Determination of Histamine Concentration |
| title | Development of a Capacitance-Based Biosensor for the Determination of Histamine Concentration |
| title_full | Development of a Capacitance-Based Biosensor for the Determination of Histamine Concentration |
| title_fullStr | Development of a Capacitance-Based Biosensor for the Determination of Histamine Concentration |
| title_full_unstemmed | Development of a Capacitance-Based Biosensor for the Determination of Histamine Concentration |
| title_short | Development of a Capacitance-Based Biosensor for the Determination of Histamine Concentration |
| title_sort | development of a capacitance based biosensor for the determination of histamine concentration |
| topic | Histamine Biosensors |
| url | http://psasir.upm.edu.my/id/eprint/7209/1/IB_2009_1a.pdf |
| work_keys_str_mv | AT wasohmohamadisahelmi developmentofacapacitancebasedbiosensorforthedeterminationofhistamineconcentration |