Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities
Epidermal mucus of fish contains a number of biologically active constituents including antimicrobial peptides that are continuously expressed and also engaged in provision of protection to the fish against injurious substances as well as potential pathogenic microbes. This study seeks to determine...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72144/1/Crude%20extracts%20of%20epidermal%20mucus%20and%20epidermis%20of%20climbing%20perch%20Anabas%20testudineus%20and%20its%20antibacterial%20and%20hemolytic%20activities.pdf |
| _version_ | 1825950037017886720 |
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| author | Al-Rasheed, Agharid Handool, Kareem Obayes Garba, Bashiru Mohamed Mustapha, Noordin Bejo, Siti Khairani Mustaffa Kamal, Farina Mohd Daud, Hassan |
| author_facet | Al-Rasheed, Agharid Handool, Kareem Obayes Garba, Bashiru Mohamed Mustapha, Noordin Bejo, Siti Khairani Mustaffa Kamal, Farina Mohd Daud, Hassan |
| author_sort | Al-Rasheed, Agharid |
| collection | UPM |
| description | Epidermal mucus of fish contains a number of biologically active constituents including antimicrobial peptides that are continuously expressed and also engaged in provision of protection to the fish against injurious substances as well as potential pathogenic microbes. This study seeks to determine the hemolytic and antibacterial properties of crude extract from epidermal mucus of climbing perch. Epidermal mucus and epidermis were obtained from 15 climbing perches after hypothermic stress stimulation for extraction of crude by aqueous and acidic methods. Antibacterial activity by well diffusion agar was applied for both extracts against 11 strains belonging to 7 different bacterial species using well agar diffusion test assay. In addition, haemolytic activity was investigated using horse RBCs. Antibacterial activity was performed for acidified crude extract only and it was found to be significant (P < 0.05) against P. aeruginosa while the least reactivity was observed against Methicillin Resistant Staphylococcus aureus. Total soluble protein concentration was 1.2 mg/ml with 13 bands of proteins (245–11) kDa distinguished. There was absence of haemolytic activity of acidified crude extract using horse RBCs. The finding of this study indicates that proteins in bioactive crude extracted from climbing perch have potential therapeutic application. |
| first_indexed | 2024-03-06T10:08:29Z |
| format | Article |
| id | upm.eprints-72144 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:08:29Z |
| publishDate | 2018 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | upm.eprints-721442020-03-04T06:33:18Z http://psasir.upm.edu.my/id/eprint/72144/ Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities Al-Rasheed, Agharid Handool, Kareem Obayes Garba, Bashiru Mohamed Mustapha, Noordin Bejo, Siti Khairani Mustaffa Kamal, Farina Mohd Daud, Hassan Epidermal mucus of fish contains a number of biologically active constituents including antimicrobial peptides that are continuously expressed and also engaged in provision of protection to the fish against injurious substances as well as potential pathogenic microbes. This study seeks to determine the hemolytic and antibacterial properties of crude extract from epidermal mucus of climbing perch. Epidermal mucus and epidermis were obtained from 15 climbing perches after hypothermic stress stimulation for extraction of crude by aqueous and acidic methods. Antibacterial activity by well diffusion agar was applied for both extracts against 11 strains belonging to 7 different bacterial species using well agar diffusion test assay. In addition, haemolytic activity was investigated using horse RBCs. Antibacterial activity was performed for acidified crude extract only and it was found to be significant (P < 0.05) against P. aeruginosa while the least reactivity was observed against Methicillin Resistant Staphylococcus aureus. Total soluble protein concentration was 1.2 mg/ml with 13 bands of proteins (245–11) kDa distinguished. There was absence of haemolytic activity of acidified crude extract using horse RBCs. The finding of this study indicates that proteins in bioactive crude extracted from climbing perch have potential therapeutic application. Elsevier 2018-06 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72144/1/Crude%20extracts%20of%20epidermal%20mucus%20and%20epidermis%20of%20climbing%20perch%20Anabas%20testudineus%20and%20its%20antibacterial%20and%20hemolytic%20activities.pdf Al-Rasheed, Agharid and Handool, Kareem Obayes and Garba, Bashiru and Mohamed Mustapha, Noordin and Bejo, Siti Khairani and Mustaffa Kamal, Farina and Mohd Daud, Hassan (2018) Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities. Egyptian Journal of Aquatic Research, 44 (2). 125 - 129. ISSN 1687-4285; ESSN: 2090-3278 https://www.sciencedirect.com/science/article/pii/S1687428518300256 10.1016/j.ejar.2018.06.002 |
| spellingShingle | Al-Rasheed, Agharid Handool, Kareem Obayes Garba, Bashiru Mohamed Mustapha, Noordin Bejo, Siti Khairani Mustaffa Kamal, Farina Mohd Daud, Hassan Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities |
| title | Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities |
| title_full | Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities |
| title_fullStr | Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities |
| title_full_unstemmed | Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities |
| title_short | Crude extracts of epidermal mucus and epidermis of climbing perch Anabas testudineus and its antibacterial and hemolytic activities |
| title_sort | crude extracts of epidermal mucus and epidermis of climbing perch anabas testudineus and its antibacterial and hemolytic activities |
| url | http://psasir.upm.edu.my/id/eprint/72144/1/Crude%20extracts%20of%20epidermal%20mucus%20and%20epidermis%20of%20climbing%20perch%20Anabas%20testudineus%20and%20its%20antibacterial%20and%20hemolytic%20activities.pdf |
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