Cytotoxicity effect of Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) on liver cancer cell integrated with hepatitis B genome, Hep3B
Thymoquinone (TQ), a bioactive compound found in Nigella sativa, cannot be orally consumed due to its lipophilicity. In order to overcome this low bioavailability, TQ is loaded into a colloidal drug carrier known as a nanostructured lipid carrier (NLC). This study aims to determine the antiprolifera...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi
2018
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| Online Access: | http://psasir.upm.edu.my/id/eprint/72165/1/Cytotoxicity%20effect%20of%20Thymoquinone.pdf |
| _version_ | 1796979412336377856 |
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| author | Haron, Aminah Suhaila Syed Alwi, Sharifah Sakinah Saiful Yazan, Latifah Abd Razak, Rohaina Ong, Yong Sze Zakarial Ansar, Fatin Hannani Alexander, Henna Roshini |
| author_facet | Haron, Aminah Suhaila Syed Alwi, Sharifah Sakinah Saiful Yazan, Latifah Abd Razak, Rohaina Ong, Yong Sze Zakarial Ansar, Fatin Hannani Alexander, Henna Roshini |
| author_sort | Haron, Aminah Suhaila |
| collection | UPM |
| description | Thymoquinone (TQ), a bioactive compound found in Nigella sativa, cannot be orally consumed due to its lipophilicity. In order to overcome this low bioavailability, TQ is loaded into a colloidal drug carrier known as a nanostructured lipid carrier (NLC). This study aims to determine the antiproliferative effects of TQ and TQ-NLC on liver cancer cells integrated with the hepatitis B genome, Hep3B. The Hep3B was treated with TQ or TQ-NLC for 24, 48, and 72 hours via MTT assay. The results confirm that TQ or TQ-NLC inhibited the growth of Hep3B at IC50 <16.7 μM for 72 hours. TQ was also found to induce cell cycle arrest at the G1 checkpoint while TQ-NLC induced non-phase-specific cell cycle arrest. Further analysis using Annexin V staining confirmed the apoptotic induction of TQ or TQ-NLC via activation of caspases-3/7. In ROS management, TQ acted as a prooxidant (increased the level of ROS), while TQ-NLC acted as an antioxidant (reduced the level of ROS). Molecular analysis demonstrated that the GSH system and the Nrf2/Keap1 signaling pathway in Hep3B influenced the differential responses of the cells towards TQ or TQ-NLC. Hence, this study demonstrated that TQ and TQ-NLC have in vitro anticancer effects on the Hep3B. |
| first_indexed | 2024-03-06T10:08:32Z |
| format | Article |
| id | upm.eprints-72165 |
| institution | Universiti Putra Malaysia |
| language | English |
| last_indexed | 2024-03-06T10:08:32Z |
| publishDate | 2018 |
| publisher | Hindawi |
| record_format | dspace |
| spelling | upm.eprints-721652020-03-05T03:33:30Z http://psasir.upm.edu.my/id/eprint/72165/ Cytotoxicity effect of Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) on liver cancer cell integrated with hepatitis B genome, Hep3B Haron, Aminah Suhaila Syed Alwi, Sharifah Sakinah Saiful Yazan, Latifah Abd Razak, Rohaina Ong, Yong Sze Zakarial Ansar, Fatin Hannani Alexander, Henna Roshini Thymoquinone (TQ), a bioactive compound found in Nigella sativa, cannot be orally consumed due to its lipophilicity. In order to overcome this low bioavailability, TQ is loaded into a colloidal drug carrier known as a nanostructured lipid carrier (NLC). This study aims to determine the antiproliferative effects of TQ and TQ-NLC on liver cancer cells integrated with the hepatitis B genome, Hep3B. The Hep3B was treated with TQ or TQ-NLC for 24, 48, and 72 hours via MTT assay. The results confirm that TQ or TQ-NLC inhibited the growth of Hep3B at IC50 <16.7 μM for 72 hours. TQ was also found to induce cell cycle arrest at the G1 checkpoint while TQ-NLC induced non-phase-specific cell cycle arrest. Further analysis using Annexin V staining confirmed the apoptotic induction of TQ or TQ-NLC via activation of caspases-3/7. In ROS management, TQ acted as a prooxidant (increased the level of ROS), while TQ-NLC acted as an antioxidant (reduced the level of ROS). Molecular analysis demonstrated that the GSH system and the Nrf2/Keap1 signaling pathway in Hep3B influenced the differential responses of the cells towards TQ or TQ-NLC. Hence, this study demonstrated that TQ and TQ-NLC have in vitro anticancer effects on the Hep3B. Hindawi 2018 Article PeerReviewed text en http://psasir.upm.edu.my/id/eprint/72165/1/Cytotoxicity%20effect%20of%20Thymoquinone.pdf Haron, Aminah Suhaila and Syed Alwi, Sharifah Sakinah and Saiful Yazan, Latifah and Abd Razak, Rohaina and Ong, Yong Sze and Zakarial Ansar, Fatin Hannani and Alexander, Henna Roshini (2018) Cytotoxicity effect of Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) on liver cancer cell integrated with hepatitis B genome, Hep3B. Evidence-Based Complementary and Alternative Medicine, 2018. art. no. 1549805. pp. 1-13. ISSN 1741-427X; ESSN: 1741-4288 https://www.hindawi.com/journals/ecam/2018/1549805/ 10.1155/2018/1549805 |
| spellingShingle | Haron, Aminah Suhaila Syed Alwi, Sharifah Sakinah Saiful Yazan, Latifah Abd Razak, Rohaina Ong, Yong Sze Zakarial Ansar, Fatin Hannani Alexander, Henna Roshini Cytotoxicity effect of Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) on liver cancer cell integrated with hepatitis B genome, Hep3B |
| title | Cytotoxicity effect of Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) on liver cancer cell integrated with hepatitis B genome, Hep3B |
| title_full | Cytotoxicity effect of Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) on liver cancer cell integrated with hepatitis B genome, Hep3B |
| title_fullStr | Cytotoxicity effect of Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) on liver cancer cell integrated with hepatitis B genome, Hep3B |
| title_full_unstemmed | Cytotoxicity effect of Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) on liver cancer cell integrated with hepatitis B genome, Hep3B |
| title_short | Cytotoxicity effect of Thymoquinone-loaded nanostructured lipid carrier (TQ-NLC) on liver cancer cell integrated with hepatitis B genome, Hep3B |
| title_sort | cytotoxicity effect of thymoquinone loaded nanostructured lipid carrier tq nlc on liver cancer cell integrated with hepatitis b genome hep3b |
| url | http://psasir.upm.edu.my/id/eprint/72165/1/Cytotoxicity%20effect%20of%20Thymoquinone.pdf |
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